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Article

Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage

  • Authors:
    • Liang Xiao
    • Shujuan Xu
    • Xiao Wang
    • Zhongxing Jin
    • Jing Wang
    • Bijing Yang
    • Hongguang Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Surgery, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China
  • Pages: 5751-5759
    |
    Published online on: October 25, 2018
       https://doi.org/10.3892/mmr.2018.9592
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Abstract

The present study aimed to verify the presence of stem cells with multilineage differentiation potential in human lumbar zygapophyseal articular cartilage (LZAC) and to compare the chondrogenic potential of cells obtained from differentially degenerated articular cartilage samples. Surgically obtained human lumbar zygapophyseal joint tissues were classified into the normal, mildly degenerated and severely degenerated groups, according to their pathological characteristics. Primary chondrocytes from these groups were cultured, and stem cells were selected using a monoclonal cell culture method. Differences in stem cell morphology between the three groups were observed using inverted microscopy and phalloidin staining. In addition, stem cell chondrogenic potential was determined through induced differentiation and cellular staining. Gene and protein expression levels of the chondrogenic‑specific markers aggrecan, collagen type‑II and SRY‑related high‑mobility‑group box 9 were determined using reverse transcription‑quantitative polymerase chain reaction and western blotting. The clonogenic ability of stem cells in the three groups was determined using a clonogenic assay. It was revealed that stem cells with multilineage differentiation potential were isolated from all three cartilage groups; however, the cells obtained from severely degenerated articular cartilage resulted in severe fibrosis, whilst those obtained from mildly degenerated articular cartilage possessed stronger chondrogenic and clonogenic abilities. Taken together, stem cells with multilineage differentiation potential and clonal properties were identified in human LZAC, and these characteristics were more prominent in mildly degenerated as compared with severely degenerated articular cartilage.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao L, Xu S, Wang X, Jin Z, Wang J, Yang B and Xu H: Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage. Mol Med Rep 18: 5751-5759, 2018.
APA
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., & Xu, H. (2018). Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage. Molecular Medicine Reports, 18, 5751-5759. https://doi.org/10.3892/mmr.2018.9592
MLA
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., Xu, H."Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage". Molecular Medicine Reports 18.6 (2018): 5751-5759.
Chicago
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., Xu, H."Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage". Molecular Medicine Reports 18, no. 6 (2018): 5751-5759. https://doi.org/10.3892/mmr.2018.9592
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao L, Xu S, Wang X, Jin Z, Wang J, Yang B and Xu H: Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage. Mol Med Rep 18: 5751-5759, 2018.
APA
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., & Xu, H. (2018). Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage. Molecular Medicine Reports, 18, 5751-5759. https://doi.org/10.3892/mmr.2018.9592
MLA
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., Xu, H."Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage". Molecular Medicine Reports 18.6 (2018): 5751-5759.
Chicago
Xiao, L., Xu, S., Wang, X., Jin, Z., Wang, J., Yang, B., Xu, H."Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage". Molecular Medicine Reports 18, no. 6 (2018): 5751-5759. https://doi.org/10.3892/mmr.2018.9592
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