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Article Open Access

lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes

  • Authors:
    • Xiaoliang Mei
    • Jian Tong
    • Wei Zhu
    • Yongliang Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Taizhou People's Hospital of Nantong University, Taizhou, Jiangsu 225300, P.R. China, Department of Orthopedics, Nanjing Central Hospital, Nanjing, Jiangsu 210018, P.R. China
    Copyright: © Mei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3867-3873
    |
    Published online on: September 2, 2019
       https://doi.org/10.3892/mmr.2019.10639
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Abstract

Osteoarthritis (OA) is one of the most prevalent types of chronic joint diseases. Chondrocytes survival is closely associated with the destruction of joints in patients with OA. Long noncoding RNAs (lncRNAs) serve a critical role in OA. However, to the best of our knowledge, the role of lncRNAs NR024118 in OA has not been examined. In the present study, the expression levels of NR024118 in lipopolysaccharide (LPS)‑induced chondrocytes was measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and the apoptosis levels of cells was determined using flow cytometry. The levels of scavenged reactive oxygen species and expression levels of the antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and heme oxygenase‑1 (HO‑1) were measured using specialized detection kits. The expression of interleukin (IL)‑1β, IL‑6 and IL‑18 were measured using ELISA. Expression of the cell apoptosis markers Bcl‑2, Bax, Bcl‑2‑like protein 11, NF‑κB, phosphorylated (p)‑NF‑κB inhibitor β (IκBβ), IκBβ, p‑transcription factor p65 (p65) and p65, and nuclear factor erythroid‑2 related factor 2 (Nrf2) signaling pathways‑associated proteins, Nrf2, HO‑1 and quinone oxidoreductase‑1 were detected by western blot analysis and RT‑qPCR. The results indicated that in ATDC5 cells, apoptosis, oxidative stress and inflammation were significantly increased and the expression level of NR024118 was significantly decreased by LPS‑mediated induction. NR024118 overexpression significantly reversed the effects of LPS treatment in the ATDC5 cell line. In addition, the overexpression of NR024118 decreased NF‑κB expression levels and activated the Nrf2 signaling pathways in LPS‑induced ATDC5 cells. The present study demonstrated that NR024118 attenuated the effects of LPS‑induction on ATDC5 cells. These results suggest that NR024118 may be a potential target for diagnosis and treatment of OA.
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1 

Tong W, Geng Y, Huang Y, Shi Y, Xiang S, Zhang N, Qin L, Shi Q, Chen Q, Dai K and Zhang X: In vivo identification and induction of articular cartilage stem cells by inhibiting NF-κB signaling in osteoarthritis. Stem Cells. 33:3125–3137. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Thomas AC, Hubbard-Turner T, Wikstrom EA and Palmieri-Smith RM: Epidemiology of posttraumatic osteoarthritis. J Athl Train. 52:491–496. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Mithoefer K: Complex articular cartilage restoration. Sports Med Arthrosc Rev. 21:31–37. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Yucesoy B, Charles LE, Baker B and Burchfiel CM: Occupational and genetic risk factors for osteoarthritis: A review. Work. 50:261–273. 2015.PubMed/NCBI

5 

Goldring MB: The role of the chondrocyte in osteoarthritis. Arthritis Rheum. 43:1916–1926. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Bay-Jensen AC, Slagboom E, Chen-An P, Alexandersen P, Qvist P, Christiansen C, Meulenbelt I and Karsdal MA: Role of hormones in cartilage and joint metabolism: Understanding an unhealthy metabolic phenotype in osteoarthritis. Menopause. 20:578–586. 2013.PubMed/NCBI

7 

Jiang SD, Lu J, Deng ZH, Li YS and Lei GH: Long noncoding RNAs in osteoarthritis. Joint Bone Spine. 84:553–556. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Ørom UA, Derrien T, Beringer M, Gumireddy K, Gardini A, Bussotti G, Lai F, Zytnicki M, Notredame C, Huang Q, et al: Long noncoding RNAs with enhancer-like function in human cells. Cell. 143:46–58. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Li F, Sun J, Huang S, Su G and Pi G: lncRNA GAS5 overexpression reverses LPS-induced inflammatory injury and apoptosis through Up-regulating KLF2 expression in ATDC5 chondrocytes. Cell Physiol Biochem. 45:1241–1251. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Shih RH, Wang CY and Yang CM: NF-kappaB signaling pathways in neurological inflammation: A mini review. Front Mol Neurosci. 8:772015. View Article : Google Scholar : PubMed/NCBI

11 

Luo C, Urgard E, Vooder T and Metspalu A: The role of COX-2 and Nrf2/ARE in anti-inflammation and antioxidative stress: Aging and anti-aging. Med Hypotheses. 77:174–178. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Ahmed SM, Luo L, Namani A, Wang XJ and Tang X: Nrf2 signaling pathway: Pivotal roles in inflammation. Biochim Biophys Acta Mol Basis Dis. 1863:585–597. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Liu Q, Niu J, Li H, Ke Y, Li R, Zhang Y and Lin J: Knee symptomatic osteoarthritis, walking disability, NSAIDs use and All-cause mortality: Population-based wuchuan osteoarthritis study. Sci Rep. 7:33092017. View Article : Google Scholar : PubMed/NCBI

15 

Saklatvala J: Inflammatory signaling in cartilage: MAPK and NF-kappaB pathways in chondrocytes and the use of inhibitors for research into pathogenesis and therapy of osteoarthritis. Curr Drug Targets. 8:305–313. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Chang CH, Hsu YM, Chen YC, Lin FH, Sadhasivam S, Loo ST and Savitha S: Anti-inflammatory effects of hydrophilic and lipophilic statins with hyaluronic acid against LPS-induced inflammation in porcine articular chondrocytes. J Orthop Res. 32:557–565. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Zhang C, Wang P, Jiang P, Lv Y, Dong C, Dai X, Tan L and Wang Z: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis. Gene. 586:248–253. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Pan L, Liu D, Zhao L, Wang L, Xin M and Li X: Long noncoding RNA MALAT1 alleviates lipopolysaccharide-induced inflammatory injury by upregulating microRNA-19b in murine chondrogenic ATDC5 cells. J Cell Biochem. 119:10165–10175. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Wang Z, Chi X, Liu L, Wang Y, Mei X, Yang Y and Jia T: Long noncoding RNA maternally expressed gene 3 knockdown alleviates lipopolysaccharide-induced inflammatory injury by up-regulation of miR-203 in ATDC5 cells. Biomed Pharmacother. 100:240–249. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Yang KY and Chen DL: Shikonin inhibits inflammatory response in rheumatoid arthritis synovial fibroblasts via lncRNA-NR024118. Evid Based Complement Alternat Med. 2015:6317372015. View Article : Google Scholar : PubMed/NCBI

21 

Tang X, Metzger D, Leeman S and Amar S: LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways. Proc Natl Acad Sci USA. 103:13777–13782. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Li L, Liu H, Shi W, Liu H, Yang J, Xu D, Huang H and Wu L: Insights into the action mechanisms of traditional Chinese medicine in osteoarthritis. Evid Based Complement Alternat Med. 2017:51909862017.PubMed/NCBI

23 

Ji B, Guo W, Ma H, Xu B, Mu W, Zhang Z, Amat A and Cao L: Isoliquiritigenin suppresses IL-1β induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-κB and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection. Int J Mol Med. 40:1709–1718. 2017.PubMed/NCBI

24 

Chen XY, Dou YX, Luo DD, Zhang ZB, Li CL, Zeng HF, Su ZR, Xie JH, Lai XP and Li YC: β-Patchoulene from patchouli oil protects against LPS-induced acute lung injury via suppressing NF-κB and activating Nrf2 pathways. Int Immunopharmacol. 50:270–278. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Jiang T, Tian F, Zheng H, Whitman SA, Lin Y, Zhang Z, Zhang N and Zhang DD: Nrf2 suppresses lupus nephritis through inhibition of oxidative injury and the NF-κB-mediated inflammatory response. Kidney Int. 85:333–343. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Wu D, Jin S, Lin Z, Chen R, Pan T, Kang X, Huang H, Lin C and Pan J: Sauchinone inhibits IL-1β induced catabolism and hypertrophy in mouse chondrocytes to attenuate osteoarthritis via Nrf2/HO-1 and NF-κB pathways. Int Immunopharmacol. 62:181–190. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Lee DF, Kuo HP, Liu M, Chou CK, Xia W, Du Y, Shen J, Chen CT, Huo L, Hsu MC, et al: KEAP1 E3 ligase-mediated downregulation of NF-kappaB signaling by targeting IKKbeta. Mol Cell. 36:131–140. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Li W, Khor TO, Xu C, Shen G, Jeong WS, Yu S and Kong AN: Activation of Nrf2-antioxidant signaling attenuates NFkappaB-inflammatory response and elicits apoptosis. Biochem Pharmacol. 76:1485–1489. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Mei X, Tong J, Zhu W and Zhu Y: lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes. Mol Med Rep 20: 3867-3873, 2019.
APA
Mei, X., Tong, J., Zhu, W., & Zhu, Y. (2019). lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes. Molecular Medicine Reports, 20, 3867-3873. https://doi.org/10.3892/mmr.2019.10639
MLA
Mei, X., Tong, J., Zhu, W., Zhu, Y."lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes". Molecular Medicine Reports 20.4 (2019): 3867-3873.
Chicago
Mei, X., Tong, J., Zhu, W., Zhu, Y."lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes". Molecular Medicine Reports 20, no. 4 (2019): 3867-3873. https://doi.org/10.3892/mmr.2019.10639
Copy and paste a formatted citation
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Spandidos Publications style
Mei X, Tong J, Zhu W and Zhu Y: lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes. Mol Med Rep 20: 3867-3873, 2019.
APA
Mei, X., Tong, J., Zhu, W., & Zhu, Y. (2019). lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes. Molecular Medicine Reports, 20, 3867-3873. https://doi.org/10.3892/mmr.2019.10639
MLA
Mei, X., Tong, J., Zhu, W., Zhu, Y."lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes". Molecular Medicine Reports 20.4 (2019): 3867-3873.
Chicago
Mei, X., Tong, J., Zhu, W., Zhu, Y."lncRNA‑NR024118 overexpression reverses LPS‑induced inflammatory injury and apoptosis via NF‑κB/Nrf2 signaling in ATDC5 chondrocytes". Molecular Medicine Reports 20, no. 4 (2019): 3867-3873. https://doi.org/10.3892/mmr.2019.10639
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