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Article

Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line

  • Authors:
    • Yongjun Chen
    • Tao Zhang
    • Xiongwei Liu
    • Zengyan Li
    • Dongming Zhou
    • Wensheng Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014010, P.R. China, Department of Immunology, Basic and Forensic Medicine of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014060, P.R. China, Department of Nephrology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia 014010, P.R. China
  • Pages: 1356-1364
    |
    Published online on: December 23, 2019
       https://doi.org/10.3892/mmr.2019.10902
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Abstract

Epithelial‑to‑mesenchymal transition (EMT) is a major process involved in tumor progression and metastasis. Melatonin is secreted by the pineal gland and has been documented as a potential therapeutic agent for multiple tumors. However, the effects of melatonin on EMT during osteosarcoma (OA) development remain undefined. The present study explored the biological functions and effects of melatonin on EMT induced by transforming growth factor β1 (TGF‑β1) and its underlying mechanisms in MG‑63 cells. Using western‑blotting and immunofluorescence, it was found that the switch in E‑cadherin/N‑cadherin and vimentin expression was induced by TGF‑β1, which was reversed by melatonin through the suppression of Snail and matrix metalloproteinase 9 (MMP‑9), through hypoxia‑inducible factor 1α (HIF‑1α) inhibition. These findings demonstrated that the anticancer effects of melatonin against OA MG‑63 cells is through the suppression of EMT via HIF‑1α/Snail/MMP‑9 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Zhang T, Liu X, Li Z, Zhou D and Xu W: Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line. Mol Med Rep 21: 1356-1364, 2020.
APA
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., & Xu, W. (2020). Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line. Molecular Medicine Reports, 21, 1356-1364. https://doi.org/10.3892/mmr.2019.10902
MLA
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., Xu, W."Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line". Molecular Medicine Reports 21.3 (2020): 1356-1364.
Chicago
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., Xu, W."Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line". Molecular Medicine Reports 21, no. 3 (2020): 1356-1364. https://doi.org/10.3892/mmr.2019.10902
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Zhang T, Liu X, Li Z, Zhou D and Xu W: Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line. Mol Med Rep 21: 1356-1364, 2020.
APA
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., & Xu, W. (2020). Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line. Molecular Medicine Reports, 21, 1356-1364. https://doi.org/10.3892/mmr.2019.10902
MLA
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., Xu, W."Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line". Molecular Medicine Reports 21.3 (2020): 1356-1364.
Chicago
Chen, Y., Zhang, T., Liu, X., Li, Z., Zhou, D., Xu, W."Melatonin suppresses epithelial‑to‑mesenchymal transition in the MG‑63 cell line". Molecular Medicine Reports 21, no. 3 (2020): 1356-1364. https://doi.org/10.3892/mmr.2019.10902
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