Open Access

Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1

  • Authors:
    • Jingwen Wu
    • Wen Ren
    • Zhichao Zheng
    • Zhu Huang
    • Tingting Liang
    • Fuyao Li
    • Zhan Shi
    • Qianzhou Jiang
    • Xuechao Yang
    • Lvhua Guo
  • View Affiliations

  • Published online on: June 22, 2020     https://doi.org/10.3892/mmr.2020.11264
  • Pages: 1737-1746
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) are a class of non‑coding RNAs that exhibit important regulatory roles in various biological processes. However, the role of circRNAs and their potential role in osteoblast differentiation and mineralization is unclear. The aim of the present study was to investigate the expression of mmu_circ_003795 and its effect on collagen type XV α 1 chain (COL15A1). First, it was identified that the expression levels of mmu_circ_003795 and osteopontin (OPN) were upregulated in the induced cells. Silencing of mmu_circ_003795 reduced the gene and protein levels of COL15A1 and OPN, whereas the expression level of mmu‑microRNA (miR)‑1249‑5p was upregulated. In addition, after 7 or 14 days of induction, alkaline phosphatase and Alizarin Red‑S staining were decreased in the mmu_circRNA_003795 inhibitory group compared with the negative control group. In conclusion, mmu_circ_003795 may regulate osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 cells via mmu‑miR‑1249‑5p by targeting COL15A1.
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September-2020
Volume 22 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Wu J, Ren W, Zheng Z, Huang Z, Liang T, Li F, Shi Z, Jiang Q, Yang X, Guo L, Guo L, et al: Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1. Mol Med Rep 22: 1737-1746, 2020
APA
Wu, J., Ren, W., Zheng, Z., Huang, Z., Liang, T., Li, F. ... Guo, L. (2020). Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1. Molecular Medicine Reports, 22, 1737-1746. https://doi.org/10.3892/mmr.2020.11264
MLA
Wu, J., Ren, W., Zheng, Z., Huang, Z., Liang, T., Li, F., Shi, Z., Jiang, Q., Yang, X., Guo, L."Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1". Molecular Medicine Reports 22.3 (2020): 1737-1746.
Chicago
Wu, J., Ren, W., Zheng, Z., Huang, Z., Liang, T., Li, F., Shi, Z., Jiang, Q., Yang, X., Guo, L."Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1". Molecular Medicine Reports 22, no. 3 (2020): 1737-1746. https://doi.org/10.3892/mmr.2020.11264