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High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma

  • Authors:
    • Xiao-Yang Hua
    • Xing-Xing Bie
    • Xi Cheng
    • Shu-Guang Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Neurology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Hua et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 12
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    Published online on: November 3, 2020
       https://doi.org/10.3892/mmr.2020.11650
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Abstract

SH3 domain‑containing kinase‑binding protein 1 (CIN85), an 85 kDa protein known to be a member of the signal adaptor family, is abnormally expressed in several human malignancies and has been found to be involved in the growth, migration and invasion of these tumors. The objective of the present study was to clarify the clinical significance of CIN85 in human esophageal squamous cell carcinoma (ESCC), as well as its in vitro functions. CIN85 expression was evaluated in 129 cases of ESCC and its adjacent normal tissues using immunohistochemistry to explore its clinical relevance and prognostic value. The functions of CIN85 in the ESCC TE1 cell line were analyzed in vitro using the interfering short hairpin RNA silencing technique. MTS, wound healing, clone formation and Transwell assays were used to detect the proliferation, migration and invasion of ESCC cells. CIN85 expression was identified mainly in ESCCs and their adjacent normal tissues, and the high expression of CIN85 was significantly associated with advanced Tumor Node Metastasis stage and lymph node metastasis. CIN85 gene silencing significantly inhibited TE1 cell proliferation, migration and invasion. These results demonstrated that CIN85 was highly expressed in advanced stage ESCC and lymph node metastasis, and played a critical role in tumor proliferation and progression. Therefore, CIN85 may be a promising therapeutic target for human ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Hua X, Bie X, Cheng X and Zhang S: High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma. Mol Med Rep 23: 12, 2021.
APA
Hua, X., Bie, X., Cheng, X., & Zhang, S. (2021). High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma. Molecular Medicine Reports, 23, 12. https://doi.org/10.3892/mmr.2020.11650
MLA
Hua, X., Bie, X., Cheng, X., Zhang, S."High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma". Molecular Medicine Reports 23.1 (2021): 12.
Chicago
Hua, X., Bie, X., Cheng, X., Zhang, S."High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma". Molecular Medicine Reports 23, no. 1 (2021): 12. https://doi.org/10.3892/mmr.2020.11650
Copy and paste a formatted citation
x
Spandidos Publications style
Hua X, Bie X, Cheng X and Zhang S: High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma. Mol Med Rep 23: 12, 2021.
APA
Hua, X., Bie, X., Cheng, X., & Zhang, S. (2021). High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma. Molecular Medicine Reports, 23, 12. https://doi.org/10.3892/mmr.2020.11650
MLA
Hua, X., Bie, X., Cheng, X., Zhang, S."High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma". Molecular Medicine Reports 23.1 (2021): 12.
Chicago
Hua, X., Bie, X., Cheng, X., Zhang, S."High expression of CIN85 promotes proliferation and invasion of human esophageal squamous cell carcinoma". Molecular Medicine Reports 23, no. 1 (2021): 12. https://doi.org/10.3892/mmr.2020.11650
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