Human galectin‑3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions <em>in vivo</em>

Gál,Peter; Vasilenko,Tomáš; Kováč,Ivan; Čoma,Matúš; Jakubčo,Ján; Jakubčová,Martina; Peržeľová,Vlasta; Urban,Lukáš; Kolář,Michal; Sabol,František; Luczy,Ján; Novotný,Martin; Majerník,Jaroslav; Gabius,Hans-Joachim; Smetana,Karel  Jr

doi:10.3892/mmr.2020.11738

Human galectin‑3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo

Authors:
- Peter Gál
- Tomáš Vasilenko
- Ivan Kováč
- Matúš Čoma
- Ján Jakubčo
- Martina Jakubčová
- Vlasta Peržeľová
- Lukáš Urban
- Michal Kolář
- František Sabol
- Ján Luczy
- Martin Novotný
- Jaroslav Majerník
- Hans-Joachim Gabius
- Karel Jr Smetana
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Affiliations: Department of Biomedical Research, East‑Slovak Institute of Cardiovascular Diseases, 040 66 Košice, Slovak Republic, Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of The Czech Academy of Sciences, 142 20 Prague, Czech Republic, Department of Heart Surgery, East‑Slovak Institute of Cardiovascular Diseases, 040 66 Košice, Slovak Republic, Department of Medical Informatics, Faculty of Medicine, Pavol Jozef Šafárik University, 040 66 Košice, Slovak Republic, Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig‑Maximilian‑University, D‑80539 Munich, Germany, Institute of Anatomy, First Faculty of Medicine, Charles University, 128 00 Prague
Published online on: November 27, 2020 https://doi.org/10.3892/mmr.2020.11738
Article Number: 99
Copyright: © Gál et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Understanding the molecular and cellular processes in skin wound healing can pave the way for devising innovative concepts by turning the identified natural effectors into therapeutic tools. Based on the concept of broad‑scale engagement of members of the family of galactoside‑binding lectins (galectins) in pathophysiological processes, such as cancer or tissue repair/regeneration, the present study investigated the potential of galectins‑1 (Gal‑1) and ‑3 (Gal‑3) in wound healing. Human dermal fibroblasts, which are key cells involved in skin wound healing, responded to galectin exposure (Gal‑1 at 300 or Gal‑3 at 600 ng/ml) with selective changes in gene expression among a panel of 84 wound‑healing‑related genes, as well as remodeling of the extracellular matrix. In the case of Gal‑3, positive expression of Ki67 and cell number increased when using a decellularized matrix produced by Gal‑3‑treated fibroblasts as substrate for culture of interfollicular keratinocytes. In vivo wounds were topically treated with 20 ng/ml Gal‑1 or ‑3, and collagen score was found to be elevated in excisional wound repair in rats treated with Gal‑3. The tensile strength measured in incisions was significantly increased from 79.5±17.5 g/mm² in controls to 103.1±21.4 g/mm² after 21 days of healing. These data warrant further testing mixtures of galectins and other types of compounds, for example a combination of galectins and TGF‑β1.

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