[Corrigendum] Anti‑fibrotic effect of Sedum sarmentosum Bunge extract in kidneys via the hedgehog signaling pathway

Figure 1.

SSBE inhibits hedgehog signaling activity and alleviates interstitial fibrosis in UUO kidneys. (A) H&E and Masson trichrome staining indicated marked kidney injury and excessive accumulation of total collagen in UUO kidneys, but SSBE administration alleviated this effect. Scale bar, 100 µm. (B) Enhanced mRNA expression levels of Col1α1, Col3α1, TGF-β1 and TGF-β1R in UUO kidneys, determined by reverse transcription quantitative polymerase chain reaction, were inhibited by SSBE treatment. (C) Immunochemical staining indicated upregulated expression of Col1α1 and TGF-β1 in UUO kidneys, which were alleviated following SSBE administration. Scale bar, 50 µm. (D) SSBE decreased PCNA expression in kidney tissues of UUO rats. Scale bar, 50 µm. (E) SSBE administration inhibited UUO-induced downregulated protein expression levels of Ptch1, and upregulated expression of Smo. Scale bar, 50 µm. (F) UUO decreased mRNA expression levels of Ptch1 and increased expression of Smo, but were inhibited by SSBE treatment. Data are presented as the mean ± standard error. *P<0.05, **P<0.01 vs. sham; #P<0.05, ##P<0.01 vs. vehicle. H&E, hematoxylin and eosin; UUO, unilateral ureteral obstruction; Col1α1, type I collagen; Col3α1, type III collagen; TGF-β1, transforming growth factor-β1; TGF-β1R, transforming growth factor β1 receptor; SSBE, Sedum sarmentosum Bunge; PCNA, proliferating cell nuclear antigen; Ptch1, protein patched homolog 1; Smo, smoothened.