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Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells

  • Authors:
    • Chunyan Xu
    • Wenlu Zhang
    • Shiquan Liu
    • Wenhong Wu
    • Mengbin Qin
    • Jiean Huang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530007, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9719-9724
    |
    Published online on: April 26, 2018
       https://doi.org/10.3892/ol.2018.8588
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Abstract

Sphingosine kinase 1 (SphK1) is a master kinase that catalyzes the synthesis of sphingosine 1 phosphate and participates in the regulation of cell proliferation and autophagy. The present study aimed to assess the effects of the activation of the SphK1/extracellular signal‑regulated kinase (ERK)/phosphorylated (p‑)ERK pathway in the regulation of autophagy in colon cancer (HT‑29) cells. Inverted fluorescence microscopy was used to detect the expression of green fluorescent protein (GFP) in the SphK1‑overexpressing HT‑29 cells [SphK1(+)‑HT‑29] and the negative control HT‑29 cells (NC‑HT‑29). Western blotting was used to detect the protein expression levels of SphK1, ERK1/2, p‑ERK1/2, as well as those of the autophagy‑associated markers LC3A, ATG5, and ULK1. Protein localization and expression of the LC3A antibody were detected by immunofluorescence. The results demonstrated that GFP was similarly expressed in SphK1(+)‑HT‑29 and NC‑HT‑29 cells. However, significantly increased SphK1 mRNA and protein expression levels were detected in SphK1(+)‑HT‑29 cells compared with in NC‑HT‑29 cells, which resulted in upregulated ERK/p‑ERK. Furthermore, the protein expression levels of the three autophagy‑associated markers increased. LC3A protein was localized in the cytoplasm of SphK1(+)‑HT‑29 cells, indicating autophagy. In summary, the findings of the present study suggested that activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer HT‑29 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xu C, Zhang W, Liu S, Wu W, Qin M and Huang J: Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells. Oncol Lett 15: 9719-9724, 2018.
APA
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., & Huang, J. (2018). Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells. Oncology Letters, 15, 9719-9724. https://doi.org/10.3892/ol.2018.8588
MLA
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., Huang, J."Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells". Oncology Letters 15.6 (2018): 9719-9724.
Chicago
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., Huang, J."Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells". Oncology Letters 15, no. 6 (2018): 9719-9724. https://doi.org/10.3892/ol.2018.8588
Copy and paste a formatted citation
x
Spandidos Publications style
Xu C, Zhang W, Liu S, Wu W, Qin M and Huang J: Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells. Oncol Lett 15: 9719-9724, 2018.
APA
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., & Huang, J. (2018). Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells. Oncology Letters, 15, 9719-9724. https://doi.org/10.3892/ol.2018.8588
MLA
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., Huang, J."Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells". Oncology Letters 15.6 (2018): 9719-9724.
Chicago
Xu, C., Zhang, W., Liu, S., Wu, W., Qin, M., Huang, J."Activation of the SphK1/ERK/p‑ERK pathway promotes autophagy in colon cancer cells". Oncology Letters 15, no. 6 (2018): 9719-9724. https://doi.org/10.3892/ol.2018.8588
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