Long non‑coding RNA transcribed from pseudogene PPIAP43 is associated with radiation sensitivity of small cell lung cancer cells Corrigendum in /10.3892/ol.2019.11202

Wang,Shilong; Yu,Jinming

doi:10.3892/ol.2019.10806

Long non‑coding RNA transcribed from pseudogene PPIAP43 is associated with radiation sensitivity of small cell lung cancer cells

Corrigendum in: /10.3892/ol.2019.11202

Authors:
- Shilong Wang
- Jinming Yu
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Affiliations: Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong 250117, P.R. China
Published online on: September 4, 2019 https://doi.org/10.3892/ol.2019.10806
Pages: 4583-4592
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Small cell lung cancer (SCLC) is a highly lethal disease. Although radiation therapy is effective for the majority of patients with SCLC, patient sensitivity to radiation varies. The lack of biomarkers impedes advances in targeting radiation‑sensitive patients. In this study, the changes in transcription patterns of SCLC cell lines were evaluated with or without 2 Gy gamma radiation. The results demonstrated that peptidyl‑prolyl cis‑trans isomerase A pseudogene 43 (PPIAP43) transcription was increased 2‑fold in cells irradiated with 2 Gy gamma radiation compared with unirradiated cells in pre‑reported radio‑sensitive sensitive cell lines H69, H128, H146, H209 and H187. These cells shared 259 upregulated and 96 downregulated RNA transcripts following radiation. Pre‑reported less sensitive cell lines H526, D53, D114 and D153 in which PPIAP43 was not upregulated 2‑fold following irradiation with 2 Gy gamma radiation compared with unirradiated cells, shared 3 upregulated and 9 downregulated RNA transcripts. The RNA transcript of PPIAP43 was aligned with the mRNA of peptidyl‑prolyl cis‑trans isomerase A (PPIA) at 2 sections (3,732 to 3,917 and 5,327 to 5,657 of the PPIA gene) and the sequences were shown to be 96 and 94% similar, respectively. Therefore, PPIAP43 may act as a sponge for microRNAs which bind with the RNA of PPIA. Therefore, PPIAP43 RNA transcription may serve as a potential biomarker of radio‑sensitivity of SCLC.

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