Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December-2020 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2020 Volume 20 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells

  • Authors:
    • Shanshan Li
    • Hui Yang
    • Min Zhao
    • Linli Gong
    • Yahong Wang
    • Zhiyong Lv
    • Yuhang Quan
    • Zhonghui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province), Kunming, Yunnan 650118, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 280
    |
    Published online on: September 23, 2020
       https://doi.org/10.3892/ol.2020.12143
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Propofol (2,6‑diisopropylphenol) is one of the most commonly used intravenous anesthetics and possesses a number of non‑anesthetic effects, including antitumor function. The aim of the present study was to elucidate the antitumor molecular mechanism of propofol on A549 and H1299 cells. A549 and H1299 cells were treated in the presence or absence of different concentrations (0, 60 or 120 µmol) of propofol for different durations (0, 24, 48 or 72 h), and proliferation was detected by MTT and colony formation assays; the protein levels of optineurin (OPTN) ubiquitination, HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), methyl‑CpG binding domain protein 3 (MBD3) and Microtubule‑associated protein 1A/1B‑light chain 3 were detected by immunoblotting or quantitative (q)PCR; the methylation state of the HACE1 gene promoter was detected by bisulfite DNA sequencing; and binding of MBD3 on HACE1 gene promoter was detected by chromatin immunoprecipitation‑qPCR. Propofol inhibited proliferation of A549 and H1299 cells and promoted HACE1‑OPTN axis‑mediated selective autophagy activity by increasing the protein expression levels of HACE1 via demethylating its promoter region. Furthermore, propofol promoted expression levels of MBD3 and binding to the ‑1,000 to ‑1 bp (transcription start site) region of HACE1 gene promoter. MBD3‑knockdown experiments indicated that propofol inhibited proliferation of A549 cells in a MBD3‑dependent manner. Thus, the findings of the present study provided a potential antitumor molecular mechanism mediated by propofol.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kodama M, Higuchi H, Ishii-Maruhama M, Nakano M, Honda-Wakasugi Y, Maeda S and Miyawaki T: Multi-drug therapy for epilepsy influenced bispectral index after a bolus propofol administration without affecting propofol's pharmacokinetics: A prospective cohort study. Sci Rep. 10:15782020.PubMed/NCBI

2 

Sona Khan M, Trenet W, Xing N, Sibley B, Abbas M, Al-Rashida M, Rauf K and Mandyam CD: A novel sulfonamide, 4-FS, reduces ethanol drinking and physical withdrawal associated with ethanol dependence. Int J Mol Sci. 21:44112020.

3 

Yoon HK, Jun K, Park SK, Ji SH, Jang YE, Yoo S, Kim JT and Kim WH: Anesthetic agents and cardiovascular outcomes of noncardiac surgery after coronary stent insertion. J Clin Med. 9:4292020.

4 

Kang Y, Saito M and Toyoda H: Molecular and regulatory mechanisms of desensitization and resensitization of GABAA receptors with a special reference to propofol/barbiturate. Int J Mol Sci. 21:5632020.

5 

Cho YJ, Nam K, Kim TK, Choi SW, Kim SJ, Hausenloy DJ and Jeon Y: Sevoflurane, propofol and carvedilol block myocardial protection by limb remote ischemic preconditioning. Int J Mol Sci. 20:2692019.

6 

Sun H and Gao D: Propofol suppresses growth, migration and invasion of A549 cells by down-regulation of miR-372. BMC Cancer. 18:12522018.PubMed/NCBI

7 

Vasileiou I, Xanthos T, Koudouna E, Perrea D, Klonaris C, Katsargyris A and Papadimitriou L: Propofol: A review of its non-anaesthetic effects. Eur J Pharmacol. 605:1–8. 2009.PubMed/NCBI

8 

Bode AM, Dong Z and Wang H: Cancer prevention and control: Alarming challenges in China. Natl Sci Rev. 3:117–127. 2016.PubMed/NCBI

9 

Xia T, Zhu Y, Mu L, Zhang ZF and Liu S: Pulmonary diseases induced by ambient ultrafine and engineered nanoparticles in twenty-first century. Natl Sci Rev. 3:416–429. 2016.PubMed/NCBI

10 

Zanoaga O, Braicu C, Jurj A, Rusu A, Buiga R and Berindan-Neagoe I: Progress in research on the role of flavonoids in lung cancer. Int J Mol Sci. 20:42912019.

11 

Loong HH, Kwan SS, Mok TS and Lau YM: Therapeutic strategies in EGFR mutant non-small cell lung cancer. Curr Treat Options Oncol. 19:582018.PubMed/NCBI

12 

Xue J, Yang J, Luo M, Cho WC and Liu X: MicroRNA-targeted therapeutics for lung cancer treatment. Expert Opin Drug Discov. 12:141–157. 2017.PubMed/NCBI

13 

Sarne V, Huter S, Braunmueller S, Rakob L, Jacobi N, Kitzwögerer M, Wiesner C, Obrist P and Seeboeck R: Promoter methylation of selected genes in non-small-cell lung cancer patients and cell lines. Int J Mol Sci. 21:45952020.

14 

Wang Q, Liu S, Zhao X, Wang Y, Tian D and Jiang W: MiR-372-3p promotes cell growth and metastasis by targeting FGF9 in lung squamous cell carcinoma. Cancer Med. 6:1323–1330. 2017.PubMed/NCBI

15 

Wang X, Li W, Zhang N, Zheng X and Jing Z: Opportunities and challenges of co-targeting epidermal growth factor receptor and autophagy signaling in non-small cell lung cancer. Oncol Lett. 18:499–506. 2019.PubMed/NCBI

16 

Wang H, Peng X, Huang Y, Xiao Y, Wang Z and Zhan L: Propofol attenuates hypoxia/reoxygenation-induced apoptosis and autophagy in HK-2 cells by inhibiting JNK activation. Yonsei Med J. 60:1195–1202. 2019.PubMed/NCBI

17 

Xue Y, Han H, Wu L, Pan B, Dong B, Yin CC, Tian Z, Liu X, Yang Y, Zhang H, et al: iASPP facilitates tumor growth by promoting mTOR-dependent autophagy in human non-small-cell lung cancer. Cell Death Dis. 8:e31502017.PubMed/NCBI

18 

Liu Z, Chen P, Gao H, Gu Y, Yang J, Peng H, Xu X, Wang H, Yang M, Liu X, et al: Ubiquitylation of autophagy receptor optineurin by HACE1 activates selective autophagy for tumor suppression. Cancer Cell. 26:106–120. 2014.PubMed/NCBI

19 

Liao SX, Sun PP, Gu YH, Rao XM, Zhang LY and Ou-Yang Y: Autophagy and pulmonary disease. Ther Adv Respir Dis. 13:17534666198905382019.PubMed/NCBI

20 

Xu YB, Du QH, Zhang MY, Yun P and He CY: Propofol suppresses proliferation, invasion and angiogenesis by down-regulating ERK-VEGF/MMP-9 signaling in Eca-109 esophageal squamous cell carcinoma cells. Eur Rev Med Pharmacol Sci. 17:2486–2494. 2013.PubMed/NCBI

21 

Xu X, Li C, Gao X, Xia K, Guo H, Li Y, Hao Z, Zhang L, Gao D, Xu C, et al: Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders. Cell Res. 28:48–68. 2018.PubMed/NCBI

22 

Li C, Han T, Guo R, Chen P, Peng C, Prag G and Hu R: An integrative synthetic biology approach to interrogating cellular ubiquitin and ufm signaling. Int J Mol Sci. 21:42312020.

23 

Longchamps RJ, Castellani CA, Yang SY, Newcomb CE, Sumpter JA, Lane J, Grove ML, Guallar E, Pankratz N, Taylor KD, et al: Evaluation of mitochondrial DNA copy number estimation techniques. PLoS One. 15:e02281662020.PubMed/NCBI

24 

Yu Z, Li Y, Han T and Liu Z: Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells. Oncol Lett. 17:4361–4368. 2019.PubMed/NCBI

25 

Li H, Liang Z, Yang J, Wang D, Wang H, Zhu M, Geng B and Xu EY: DAZL is a master translational regulator of murine spermatogenesis. Natl Sci Rev. 6:455–468. 2019.PubMed/NCBI

26 

Li C, Lu W, Yang L, Li Z, Zhou X, Guo R, Wang J, Wu Z, Dong Z, Ning G, et al: MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3. Natl Sci Rev. 7:671–685. 2020.

27 

Hsing CH, Lin MC, Choi PC, Huang WC, Kai JI, Tsai CC, Cheng YL, Hsieh CY, Wang CY, Chang YP, et al: Anesthetic propofol reduces endotoxic inflammation by inhibiting reactive oxygen species-regulated Akt/IKKβ/NF-κB signaling. PLoS One. 6:e175982011.PubMed/NCBI

28 

Cui D, Wang L, Qi A, Zhou Q, Zhang X and Jiang W: Propofol prevents autophagic cell death following oxygen and glucose deprivation in PC12 cells and cerebral ischemia-reperfusion injury in rats. PLoS One. 7:e353242012.PubMed/NCBI

29 

Zhang L, Wang N, Zhou S, Ye W, Jing G and Zhang M: Propofol induces proliferation and invasion of gallbladder cancer cells through activation of Nrf2. J Exp Clin Cancer Res. 31:662012.PubMed/NCBI

30 

Du Q, Liu J, Zhang X, Zhang X, Zhu H, Wei M and Wang S: Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells. Braz J Med Biol Res. 51:e68032018.PubMed/NCBI

31 

Liu WZ and Liu N: Propofol inhibits lung cancer A549 cell growth and epithelial-mesenchymal transition process by upregulation of MicroRNA-1284. Oncol Res. 27:1–8. 2018.PubMed/NCBI

32 

Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu GL and Hu R: A CRISPR-based approach for targeted DNA demethylation. Cell Discov. 2:160092016.PubMed/NCBI

33 

Brown SE, Suderman MJ, Hallett M and Szyf M: DNA demethylation induced by the methyl-CpG-binding domain protein MBD3. Gene. 420:99–106. 2008.PubMed/NCBI

34 

Peng L, Li Y, Xi Y, Li W, Li J, Lv R, Zhang L, Zou Q, Dong S, Luo H, et al: MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation. J Cell Sci. 129:1059–1071. 2016.PubMed/NCBI

35 

Brown SE and Szyf M: Epigenetic programming of the rRNA promoter by MBD3. Mol Cell Biol. 27:4938–4952. 2007.PubMed/NCBI

36 

Lee CW, Wilfling F, Ronchi P, Allegretti M, Mosalaganti S, Jentsch S, Beck M and Pfander B: Selective autophagy degrades nuclear pore complexes. Nat Cell Biol. 22:159–166. 2020.PubMed/NCBI

37 

Yamasaki A, Alam JM, Noshiro D, Hirata E, Fujioka Y, Suzuki K, Ohsumi Y and Noda NN: Liquidity is a critical determinant for selective autophagy of protein condensates. Mol Cell. 77:1163–1175.e9. 2020.PubMed/NCBI

38 

Zhao ZQ, Yu ZY, Li J and Ouyang XN: Gefitinib induces lung cancer cell autophagy and apoptosis via blockade of the PI3K/AKT/mTOR pathway. Oncol Lett. 12:63–68. 2016.PubMed/NCBI

39 

Ren S, Ding C and Sun Y: Morphology remodeling and selective autophagy of intracellular organelles during viral infections. Int J Mol Sci. 21:36892020.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li S, Yang H, Zhao M, Gong L, Wang Y, Lv Z, Quan Y and Wang Z: Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells. Oncol Lett 20: 280, 2020.
APA
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z. ... Wang, Z. (2020). Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells. Oncology Letters, 20, 280. https://doi.org/10.3892/ol.2020.12143
MLA
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z., Quan, Y., Wang, Z."Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells". Oncology Letters 20.6 (2020): 280.
Chicago
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z., Quan, Y., Wang, Z."Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells". Oncology Letters 20, no. 6 (2020): 280. https://doi.org/10.3892/ol.2020.12143
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Yang H, Zhao M, Gong L, Wang Y, Lv Z, Quan Y and Wang Z: Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells. Oncol Lett 20: 280, 2020.
APA
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z. ... Wang, Z. (2020). Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells. Oncology Letters, 20, 280. https://doi.org/10.3892/ol.2020.12143
MLA
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z., Quan, Y., Wang, Z."Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells". Oncology Letters 20.6 (2020): 280.
Chicago
Li, S., Yang, H., Zhao, M., Gong, L., Wang, Y., Lv, Z., Quan, Y., Wang, Z."Demethylation of <em>HACE1</em> gene promoter by propofol promotes autophagy of human A549&nbsp;cells". Oncology Letters 20, no. 6 (2020): 280. https://doi.org/10.3892/ol.2020.12143
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team