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KRAS and NRAS mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation

  • Authors:
    • Elena Lastraioli
    • Lorenzo Antonuzzo
    • Beatrice Fantechi
    • Luisa Di Cerbo
    • Alessandro Di Costanzo
    • Daniele Lavacchi
    • Miriam Armenio
    • Annarosa Arcangeli
    • Francesca Castiglione
    • Luca Messerini
    • Francesco Di Costanzo
  • View Affiliations / Copyright

    Affiliations: Department of Experimental and Clinical Medicine, University of Florence, I-50134 Florence, Italy, Medical Oncology Unit, Azienda Ospedaliero‑Universitaria Careggi, I-50134 Florence, Italy, Section of Pathological Anatomy, Department of Health Sciences, University of Florence, Azienda Ospedaliero‑Universitaria Careggi, I-50134 Florence, Italy
    Copyright: © Lastraioli et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 15
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    Published online on: November 6, 2020
       https://doi.org/10.3892/ol.2020.12276
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Abstract

Patients with metastatic colorectal cancer (mCRC) are routinely screened for either K‑ and N‑RAS to select the appropriate treatment. The present study aimed to evaluate the concordance between K‑ and NRAS status in the tissue (either primary tumor or metastasis) and the plasma of patients with mCRC and to identify the associations between K‑ and NRAS mutations in ctDNA and the clinicopathological parameters. Samples from a total of 31 patients with mCRC with measurable disease according to the Response Evaluation Criteria in Solid Tumors were analyzed. For all patients, K‑ and NRAS status was determined in the tissue by matrix‑assisted laser desorption/ionization time of flight mass spectrometry. For the detection of RAS mutations in cell‑free tumor DNA also defined as circulating tumor DNA (ctDNA), the OncoBEAM® RAS CRC kit (Sysmex Inostics) was used. A total of 6/31 tissue samples expressed wild‑type KRAS, whereas 25/31 presented mutations. In addition, 7/31 plasma samples expressed wild‑type KRAS, mutations were detected in 22/31 patients, and for 2/31 patients, the test did not provide a conclusive result. A total of 24/31 patients expressed wild‑type NRAS, 6/31 had mutations and 1/21 was not informative. For the KRAS mutational status, a moderate concordance (agreement, 85.18%; Cohen's k, 0.513) between the tissue and plasma analysis was observed; for NRAS, a fair agreement (agreement, 83.33%; Cohen's k, 0.242) was obtained. In conclusion, both tissue and plasma analyses should be performed for the management of patients with mCRC. To better exploit the beads, emulsions, amplification, magnetics (BEAMing) technique in the clinical setting, studies aimed at determining the RAS status to monitor therapy and during follow‑up are warranted.
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Copy and paste a formatted citation
Spandidos Publications style
Lastraioli E, Antonuzzo L, Fantechi B, Di Cerbo L, Di Costanzo A, Lavacchi D, Armenio M, Arcangeli A, Castiglione F, Messerini L, Messerini L, et al: <em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation. Oncol Lett 21: 15, 2021.
APA
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D. ... Di Costanzo, F. (2021). <em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation. Oncology Letters, 21, 15. https://doi.org/10.3892/ol.2020.12276
MLA
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D., Armenio, M., Arcangeli, A., Castiglione, F., Messerini, L., Di Costanzo, F."<em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation". Oncology Letters 21.1 (2021): 15.
Chicago
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D., Armenio, M., Arcangeli, A., Castiglione, F., Messerini, L., Di Costanzo, F."<em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation". Oncology Letters 21, no. 1 (2021): 15. https://doi.org/10.3892/ol.2020.12276
Copy and paste a formatted citation
x
Spandidos Publications style
Lastraioli E, Antonuzzo L, Fantechi B, Di Cerbo L, Di Costanzo A, Lavacchi D, Armenio M, Arcangeli A, Castiglione F, Messerini L, Messerini L, et al: <em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation. Oncol Lett 21: 15, 2021.
APA
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D. ... Di Costanzo, F. (2021). <em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation. Oncology Letters, 21, 15. https://doi.org/10.3892/ol.2020.12276
MLA
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D., Armenio, M., Arcangeli, A., Castiglione, F., Messerini, L., Di Costanzo, F."<em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation". Oncology Letters 21.1 (2021): 15.
Chicago
Lastraioli, E., Antonuzzo, L., Fantechi, B., Di Cerbo, L., Di Costanzo, A., Lavacchi, D., Armenio, M., Arcangeli, A., Castiglione, F., Messerini, L., Di Costanzo, F."<em>KRAS</em> and <em>NRAS</em> mutation detection in circulating DNA from patients with metastatic colorectal cancer using BEAMing assay: Concordance with standard biopsy and clinical evaluation". Oncology Letters 21, no. 1 (2021): 15. https://doi.org/10.3892/ol.2020.12276
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