lncRNA SNHG3 acts as oncogene in ovarian cancer through miR‑139‑5p and Notch1

Zhang,Li; Li,Guihua; Wang,Xiuzhen; Zhang,Youli; Huang,Xia; Wu,Huazhen

doi:10.3892/ol.2020.12383

lncRNA SNHG3 acts as oncogene in ovarian cancer through miR‑139‑5p and Notch1

Authors:
- Li Zhang
- Guihua Li
- Xiuzhen Wang
- Youli Zhang
- Xia Huang
- Huazhen Wu
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Affiliations: The Obstetric Ward, Jinan Maternal and Child Health Care Hospital, Jinan, Shandong 250001, P.R. China, Department of Clinical Laboratory, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China, Department of Clinical Nutrition, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Cardiology, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Infectious Department, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Gynaecology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
Published online on: December 17, 2020 https://doi.org/10.3892/ol.2020.12383
Article Number: 122
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ovarian cancer (OC) is a common malignant tumor of the female reproductive system. Long non‑coding RNAs (lncRNAs) play an important role in OC occurrence and development. Thus, the function and potential mechanism of lncRNA small nucleolar RNA host gene 3 (SNHG3) was explored in the development of OC. The expression of SNHG3, microRNA (miR)‑139‑5p and Notch homolog 1, translocation-associated (Drosophila) (Notch1) in OC were detected by RT‑qPCR or western blot assay. In addition, CCK‑8 and wound‑healing assays were used to detect OVCAR3 proliferation and migration ability. The targeting relationship of miR‑139‑5p with SNHG3 or Notch1 was verified through luciferase reporter assay. Rescue experiments were performed to confirm whether SNHG3 could mediate OVCAR3 proliferation and migration through miR‑139‑5p and Notch1. In OC tissues and cell lines, the expression of SNHG3 and Notch1 were significantly increased, and the expression of miR‑139‑5p was significantly decreased. SNHG3 inhibition suppressed the proliferation and migration of OVCAR3 cells. Luciferase reporter experiment confirmed that miR‑139‑5p could target SNHG3 and Notch1. Transfection of miR‑139‑5p inhibitor significantly reversed the inhibitory effect of SNHG3 knockdown on OVCAR3 proliferation and migration. Moreover, SNHG3 inhibition or miR‑139‑5p mimic abolished the promotion of Notch1 overexpression on OVCAR3 proliferation and migration. In conclusion, SNHG3 could accelerate the proliferation and migration of OC cells by regulating miR‑139‑5p and Notch1.

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