Annexin A1 is a potential biomarker of bone metastasis in small cell lung cancer

Chen,Peng; Min,Jie; Wu,Hong; Zhang,Helong; Wang,Chaoli; Tan,Guangguo; Zhang,Feng

doi:10.3892/ol.2020.12402

Annexin A1 is a potential biomarker of bone metastasis in small cell lung cancer

Authors:
- Peng Chen
- Jie Min
- Hong Wu
- Helong Zhang
- Chaoli Wang
- Guangguo Tan
- Feng Zhang
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Affiliations: Department of Pharmacy, Air Force Military Medical University, Xi'an, Shaanxi 710000, P.R. China, Department of Oncology, Tangdu Hospital, Air Force Military Medical University, Xi'an, Shaanxi 710000, P.R. China
Published online on: December 20, 2020 https://doi.org/10.3892/ol.2020.12402
Article Number: 141
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Small cell lung cancer (SCLC) is a subtype of lung cancer with a poor prognosis, with bone metastasis being one of the main causes of treatment failure. Therefore, investigating new biomarkers associated with bone metastasis may result in positive treatment outcomes. The present study detected the expression levels of annexin A1 (ANXA1) in the serum of 82 patients with SCLC using ELISA. ANXA1 expression in patients with SCLC with bone metastasis was significantly higher compared with that in patients without bone metastasis. Receiver operating characteristic analysis revealed that ANXA1 expression was significant in the diagnosis of bone metastasis in SCLC. ANXA1 was inhibited in SBC‑5 cells and overexpressed in SBC‑3 cells. Results revealed that ANXA1 was able to enhance SCLC cell proliferation, invasion, migration and bone adhesion in vitro. In vivo xenograft bone metastasis assays indicated that ANXA1 had the potential to promote the bone‑metastasis ability of SCLC cells in NOD/SCID mice. Furthermore, ANXA1 increased parathyroid hormone‑related protein secretion and enhanced Smad2 phosphorylation following TGF‑β treatment in SCLC cells. Overall, ANXA1 may be involved in the pathogenesis of bone metastasis in SCLC and may be a potential biomarker for the diagnosis of SCLC.

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