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Article Open Access

Dermatopontin as a potential pathogenic factor in endometrial cancer

  • Authors:
    • Haiyun Huang
    • Zhixiang Hao
    • Lingyan Long
    • Zeyuan Yin
    • Chenyu Wu
    • Xueyan Zhou
    • Bei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynaecology, Xuzhou Central Hospital, Affiliated Xuzhou Clinical College of Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 408
    |
    Published online on: March 22, 2021
       https://doi.org/10.3892/ol.2021.12669
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Abstract

The present study aimed to determine the differential expression profiles of proteins in endometrial carcinoma and to screen the proteins associated with the occurrence and development of endometrial cancer (EC). In total, 15 samples of human EC and paracancerous tissues were selected for proteomic analysis using a label‑free quantification method based on liquid chromatography‑tandem mass spectrometry. The differential proteins were analysed using bioinformatics and verified using reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. Finally, the expression of differential proteins in 75 endometrial carcinoma samples and 30 normal endometrial tissue samples were detected using immunohistochemical staining, and the associations between differential protein expression and clinicopathological features were analysed. In total, 579 up‑regulated proteins and 346 down‑regulated proteins were identified between the two groups and seven proteins with the most significant differences were selected; these proteins included interferon‑induced protein with tetratricopeptide repeats 3, poly(ADP‑ribose) polymerase family member 9, solute carrier family 34 member 2, cytochrome b5 reductase 1, protein tyrosine phosphatase non‑receptor type 1, dermatopontin (DPT) and secretory leukocyte peptidase inhibitor. RT‑qPCR and western blotting showed that DPT expression was down‑regulated (P<0.001), which was consistent with the mass spectrometry results. The immunohistochemical staining results showed that the positive expression of DPT in EC and normal endometrial tissues was statistically significant (P<0.001). The positive expression of DPT was significantly decreased in poorly differentiated, late stage, lymph node metastasis and myometrial invasion depth ≥1/2 samples (P<0.05). DPT expression was significantly lower in EC, which might play role in the pathogenesis of EC.
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Copy and paste a formatted citation
Spandidos Publications style
Huang H, Hao Z, Long L, Yin Z, Wu C, Zhou X and Zhang B: Dermatopontin as a potential pathogenic factor in endometrial cancer. Oncol Lett 21: 408, 2021.
APA
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., & Zhang, B. (2021). Dermatopontin as a potential pathogenic factor in endometrial cancer. Oncology Letters, 21, 408. https://doi.org/10.3892/ol.2021.12669
MLA
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., Zhang, B."Dermatopontin as a potential pathogenic factor in endometrial cancer". Oncology Letters 21.5 (2021): 408.
Chicago
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., Zhang, B."Dermatopontin as a potential pathogenic factor in endometrial cancer". Oncology Letters 21, no. 5 (2021): 408. https://doi.org/10.3892/ol.2021.12669
Copy and paste a formatted citation
x
Spandidos Publications style
Huang H, Hao Z, Long L, Yin Z, Wu C, Zhou X and Zhang B: Dermatopontin as a potential pathogenic factor in endometrial cancer. Oncol Lett 21: 408, 2021.
APA
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., & Zhang, B. (2021). Dermatopontin as a potential pathogenic factor in endometrial cancer. Oncology Letters, 21, 408. https://doi.org/10.3892/ol.2021.12669
MLA
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., Zhang, B."Dermatopontin as a potential pathogenic factor in endometrial cancer". Oncology Letters 21.5 (2021): 408.
Chicago
Huang, H., Hao, Z., Long, L., Yin, Z., Wu, C., Zhou, X., Zhang, B."Dermatopontin as a potential pathogenic factor in endometrial cancer". Oncology Letters 21, no. 5 (2021): 408. https://doi.org/10.3892/ol.2021.12669
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