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miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network

  • Authors:
    • Yanqin Sun
    • Yanmei Yi
    • Siyuan Gan
    • Ruifang Ye
    • Cailing Huang
    • Man Li
    • Jian Huang
    • Ying Guo
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Department of Histology and Embryology, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China, Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 459
    |
    Published online on: April 8, 2021
       https://doi.org/10.3892/ol.2021.12720
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Abstract

Numerous studies have suggested that non‑coding RNAs mediate tumorigenesis via the epithelial‑mesenchymal transition (EMT). However, whether the long non‑coding RNA (lncRNA) HOXA transcript at the distal tip (HOTTIP) plays a role in the EMT of small cell lung cancer (SCLC) remains unclear. The results of the present study suggest that HOTTIP‑knockdown may lead to a significant increase in E‑cadherin expression and a decrease in vimentin (VIM) expression; these proteins are two key markers of EMT. Furthermore, a notable morphological change in SCLC cells with HOTTIP‑knockdown was observed: After upregulation of microRNA (miR)‑574‑5p, the cells exhibited a long, fusiform morphology. Investigating these phenomena further revealed that HOTTIP may participate in EMT by binding to miR‑574‑5p. In addition, using bioinformatics technology and a dual luciferase reporter assay, it was found that miR‑574‑5p inhibited VIM expression via direct binding and interaction. In summary, the present results indicate that HOTTIP may be involved in the EMT of SCLC by binding to miR‑574‑5p, and that miR‑574‑5p may act through VIM, which is a key marker of EMT.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Yi Y, Gan S, Ye R, Huang C, Li M, Huang J and Guo Y: miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network. Oncol Lett 21: 459, 2021.
APA
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M. ... Guo, Y. (2021). miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network. Oncology Letters, 21, 459. https://doi.org/10.3892/ol.2021.12720
MLA
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M., Huang, J., Guo, Y."miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network". Oncology Letters 21.6 (2021): 459.
Chicago
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M., Huang, J., Guo, Y."miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network". Oncology Letters 21, no. 6 (2021): 459. https://doi.org/10.3892/ol.2021.12720
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Yi Y, Gan S, Ye R, Huang C, Li M, Huang J and Guo Y: miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network. Oncol Lett 21: 459, 2021.
APA
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M. ... Guo, Y. (2021). miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network. Oncology Letters, 21, 459. https://doi.org/10.3892/ol.2021.12720
MLA
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M., Huang, J., Guo, Y."miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network". Oncology Letters 21.6 (2021): 459.
Chicago
Sun, Y., Yi, Y., Gan, S., Ye, R., Huang, C., Li, M., Huang, J., Guo, Y."miR‑574‑5p mediates epithelial‑mesenchymal transition in small cell lung cancer by targeting vimentin via a competitive endogenous RNA network". Oncology Letters 21, no. 6 (2021): 459. https://doi.org/10.3892/ol.2021.12720
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