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Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation

  • Authors:
    • Min Luo
    • Yuqian Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Xiangya Hospital, Changsha, Hunan 410011, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 714
    |
    Published online on: August 6, 2021
       https://doi.org/10.3892/ol.2021.12975
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Abstract

Colorectal cancer (CRC) is one of the most common malignancies worldwide. Via analysis using The Cancer Genome Atlas database, the present study identified 1,835 genes that were differentially expressed in CRC, including 811 upregulated and 1,024 downregulated genes. Enrichment analyses using the Database for Annotation, Visualization and Integrated Discovery tool revealed that these differentially expressed genes were associated with the regulation of CRC progression by modulating multiple pathways, such as ‘Cell Cycle, Mitotic’, ‘DNA Replication’, ‘Mitotic M‑M/G1 phases’ and ‘ATM pathway’. To identify the key genes in CRC, protein‑protein interaction (PPI) network analysis was performed and the hub modules in upregulated and downregulated PPI networks were identified. Ubiquitin‑conjugating enzyme E2 T (UBE2T), a member of the E2 family, was identified to be a key regulator in CRC. To the best of our knowledge, the present study was the first to demonstrate that UBE2T expression was upregulated in CRC samples compared with normal tissues. Kaplan‑Meier analysis revealed that higher expression levels of UBE2T were associated with worse prognosis compared with lower UBE2T expression levels in CRC. Additionally, the present study demonstrated that knockdown of UBE2T inhibited CRC cell proliferation. Flow cytometry assays revealed that UBE2T knockdown induced cell cycle arrest at G1 phase and apoptosis in vitro. These results suggested that UBE2T may be a novel potential biomarker for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Luo M and Zhou Y: Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation. Oncol Lett 22: 714, 2021.
APA
Luo, M., & Zhou, Y. (2021). Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation. Oncology Letters, 22, 714. https://doi.org/10.3892/ol.2021.12975
MLA
Luo, M., Zhou, Y."Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation". Oncology Letters 22.4 (2021): 714.
Chicago
Luo, M., Zhou, Y."Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation". Oncology Letters 22, no. 4 (2021): 714. https://doi.org/10.3892/ol.2021.12975
Copy and paste a formatted citation
x
Spandidos Publications style
Luo M and Zhou Y: Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation. Oncol Lett 22: 714, 2021.
APA
Luo, M., & Zhou, Y. (2021). Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation. Oncology Letters, 22, 714. https://doi.org/10.3892/ol.2021.12975
MLA
Luo, M., Zhou, Y."Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation". Oncology Letters 22.4 (2021): 714.
Chicago
Luo, M., Zhou, Y."Comprehensive analysis of differentially expressed genes reveals the promotive effects of UBE2T on colorectal cancer cell proliferation". Oncology Letters 22, no. 4 (2021): 714. https://doi.org/10.3892/ol.2021.12975
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