Suppressive effect of YY1‑mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma

Cao,Shou-Ji; Cao,Shou-Ji; Cao,Shou-Ji; Ge,Wan-Li; Ge,Wan-Li; Ge,Wan-Li; Meng,Ling-Dong; Meng,Ling-Dong; Meng,Ling-Dong; Chen,Qun; Chen,Qun; Chen,Qun; Miao,Yi; Miao,Yi; Miao,Yi; Jiang,Kui-Rong; Jiang,Kui-Rong; Jiang,Kui-Rong; Zhang,Jing-Jing; Zhang,Jing-Jing; Zhang,Jing-Jing

doi:10.3892/ol.2022.13577

Suppressive effect of YY1‑mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma

Authors:
- Shou-Ji Cao
- Wan-Li Ge
- Ling-Dong Meng
- Qun Chen
- Yi Miao
- Kui-Rong Jiang
- Jing-Jing Zhang
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Affiliations: Pancreas Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: November 2, 2022 https://doi.org/10.3892/ol.2022.13577
Article Number: 457
Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Regulator of G‑protein signaling 22 (RGS22) is specifically expressed in the testis and in tumors of epithelial origin, but the expression and role of RGS22 in pancreatic cancer are unclear. In this study, 52 pairs of pancreatic ductal adenocarcinoma (PDAC) and adjacent non‑neoplastic tissue samples with the corresponding clinical data were used to examine the expression of RGS22 and its relationship with PDAC prognosis. The findings showed that the expression of RGS22 was higher in the PDAC tissues than in the adjacent non‑tumorous tissues and its expression was associated with the degree of blood vessel invasion. The in vitro experiments with PDAC cell lines and a normal control cell line showed that the proliferation, invasion, and metastasis of PDAC cells were suppressed by RGS22 overexpression and enhanced by RGS22 knockdown. The in vivo effect of RGS22 on PDAC xenografts was studied using subcutaneous implantation of tumor cells in BALB/cA‑nu mice, and the results corroborated the in vitro findings. Analysis of the regulators of RGS22 showed that it was positively regulated by the transcription factor Yin Yang‑1 (YY1). Thus, YY1‑mediated RGS22 regulation suppressed the proliferation, migration, and invasion of PDAC.

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