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Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate

  • Authors:
    • Saleh Abu‑Lafi
    • Mizied Falah
    • Mouhammad Zeidan
    • Mahmoud Rayan
    • Anwar Rayan
  • View Affiliations / Copyright

    Affiliations: Faculty of Pharmacy, Al‑Quds University, Abu‑Deis 144, Palestine, Research Center, Holy Family Hospital, Nazareth 1600000, Israel, Molecular Genetics and Virology Laboratory, Qasemi Research Center, Faculty of Science, Al‑Qasemi Academic College, Baka El‑Garbiah 3010000, Israel, Geriatric Department, Holy Family Hospital, Nazareth 16000, Israel, Department of Science and Technology, Faculty of Science, Al‑Qasemi Academic College, Baka El‑Garbiah 3010000, Israel
    Copyright: © Abu‑Lafi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 216
    |
    Published online on: March 5, 2025
       https://doi.org/10.3892/ol.2025.14962
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Abstract

Paclitaxel (PTX) is among the most widely used antimicrotubular chemotherapy agents available from natural sources. It has a wide range of antitumor effectiveness, particularly against breast, ovarian and lung malignancies. IDD‑1040 is a novel anticancer chemical conjugate that combines lipoic acid with PTX and demonstrates an anticancer efficiency superior to that of PTX alone. The aim of the present study was to investigate the analytical, formulation and pharmacokinetic aspects of IDD‑1040, shedding light on its pharmacological behavior and the possible mechanisms underlying its enhanced anticancer activity. IDD‑1040 was administered to mice as an intravenous bolus, and the pharmacokinetic parameters were determined over the following 7 days. The results revealed a total clearance of 1.689 l/h.kg, volume of distribution of 1.93 l/kg, average half‑life of 1.14 h and terminal half‑life of 8.64 h. Notably, the area under the curve of IDD‑1040 was >14‑fold higher than that of PTX, suggesting slower metabolism and that prodrug itself may have antitumor activity. An in vitro tubulin polymerization assay revealed distinct tubulin‑binding characteristics for IDD‑1040 compared with PTX. Due to the poor water solubility of IDD‑1040, a formulation development experiment was conducted. In total, 31 formulations were prepared that became transparent when diluted with water. In addition, some formulations achieved a relatively high drug content (12 mg/g) without the use of surfactants. Moreover, they included fewer excipients compared with the formulations diluted with water, suggesting a promising approach for drug formulation. In summary, IDD‑1040 exhibited extended circulation, efficient tissue distribution and reduced metabolite formation in vitro, warranting further exploration of its mechanisms of action and therapeutic potential. Future studies are recommended to assess the stability, pharmacokinetics and pharmacodynamics of these refined IDD‑1040 formulations to gauge their suitability for clinical application.
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Copy and paste a formatted citation
Spandidos Publications style
Abu‑Lafi S, Falah M, Zeidan M, Rayan M and Rayan A: Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate. Oncol Lett 29: 216, 2025.
APA
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., & Rayan, A. (2025). Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate. Oncology Letters, 29, 216. https://doi.org/10.3892/ol.2025.14962
MLA
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., Rayan, A."Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate". Oncology Letters 29.5 (2025): 216.
Chicago
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., Rayan, A."Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate". Oncology Letters 29, no. 5 (2025): 216. https://doi.org/10.3892/ol.2025.14962
Copy and paste a formatted citation
x
Spandidos Publications style
Abu‑Lafi S, Falah M, Zeidan M, Rayan M and Rayan A: Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate. Oncol Lett 29: 216, 2025.
APA
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., & Rayan, A. (2025). Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate. Oncology Letters, 29, 216. https://doi.org/10.3892/ol.2025.14962
MLA
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., Rayan, A."Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate". Oncology Letters 29.5 (2025): 216.
Chicago
Abu‑Lafi, S., Falah, M., Zeidan, M., Rayan, M., Rayan, A."Expanding paclitaxel's therapeutic window: Investigating the pharmacokinetic, clinical formulation and mechanistic aspects of paclitaxel‑lipoate conjugate". Oncology Letters 29, no. 5 (2025): 216. https://doi.org/10.3892/ol.2025.14962
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