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Article

Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells

  • Authors:
    • Marco Donia
    • Katia Mangano
    • Paolo Fagone
    • Rocco De Pasquale
    • Franco Dinotta
    • Marinella Coco
    • Julio Padron
    • Yousef Al-Abed
    • Giuseppe Angelo Giovanni Lombardo
    • Danijela Maksimovic-Ivanic
    • Sanja Mijatovic
    • Mai-Britt Zocca
    • Vincenzo Perciavalle
    • Stanislava Stosic-Grujicic
    • Ferdinando Nicoletti
  • View Affiliations / Copyright

    Affiliations: Department of Bio-Medical Sciences, University of Catania, Catania, Italy, Department of Dermatology Clinic, University of Catania, Catania, Italy, NicOx Research Institute, Bresso, Milan, Italy, Feinsten Institute for Medical Research, Laboratory of Medicinal Chemistry, North Shore Long Island Jewish Health System, Manhasset, NY, USA, Department of Plastic and Reconstructive Surgery, Cannizzaro Hospital, University of Catania, Catania, Italy, Department of Immunology, Institute for Biological Research ‘Sinisa Stankovic’, Belgrade University, Belgrade, Serbia, Onconox Aps, Copenhagen, Denmark
  • Pages: 682-688
    |
    Published online on: May 29, 2012
       https://doi.org/10.3892/or.2012.1840
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Abstract

We have recently shown that covalent attachment of the nitric oxide (NO) moiety to the HIV protease inhibitor Saquinavir (Saq) produced a qualitatively new chemical entity, named Saquinavir-NO (Saq-NO), with enhanced anticancer properties and reduced toxicity both in vitro and in vivo. The aim of this study was to address several unanswered questions both on the pharmacological profile of Saq-NO as well as on the in vivo role of NO in the oncogenesis of A375 human melanoma cells. To this end, we have evaluated here the impact of single and combined effects of Saq-NO, Saq, the NO-donor DETA NONOate and the iNOS inhibitor L-NAME on the in vitro as well as in vivo growth of the iNOS positive A375 cells. Our data confirm clear-cut evidence for a strong and powerful anti-melanoma action of Saq-NO that is not duplicable by the combined use of Saq and DETA NONOate. Surprisingly, but also in agreement with the complex and multifaceted role of endogenous NO in A375 cells, both DETA NONOate and L-NAME significantly suppressed the in vivo growth of xenotransplants.
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Copy and paste a formatted citation
Spandidos Publications style
Donia M, Mangano K, Fagone P, De Pasquale R, Dinotta F, Coco M, Padron J, Al-Abed Y, Giovanni Lombardo GA, Maksimovic-Ivanic D, Maksimovic-Ivanic D, et al: Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells. Oncol Rep 28: 682-688, 2012.
APA
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M. ... Nicoletti, F. (2012). Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells. Oncology Reports, 28, 682-688. https://doi.org/10.3892/or.2012.1840
MLA
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M., Padron, J., Al-Abed, Y., Giovanni Lombardo, G. A., Maksimovic-Ivanic, D., Mijatovic, S., Zocca, M., Perciavalle, V., Stosic-Grujicic, S., Nicoletti, F."Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells". Oncology Reports 28.2 (2012): 682-688.
Chicago
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M., Padron, J., Al-Abed, Y., Giovanni Lombardo, G. A., Maksimovic-Ivanic, D., Mijatovic, S., Zocca, M., Perciavalle, V., Stosic-Grujicic, S., Nicoletti, F."Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells". Oncology Reports 28, no. 2 (2012): 682-688. https://doi.org/10.3892/or.2012.1840
Copy and paste a formatted citation
x
Spandidos Publications style
Donia M, Mangano K, Fagone P, De Pasquale R, Dinotta F, Coco M, Padron J, Al-Abed Y, Giovanni Lombardo GA, Maksimovic-Ivanic D, Maksimovic-Ivanic D, et al: Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells. Oncol Rep 28: 682-688, 2012.
APA
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M. ... Nicoletti, F. (2012). Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells. Oncology Reports, 28, 682-688. https://doi.org/10.3892/or.2012.1840
MLA
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M., Padron, J., Al-Abed, Y., Giovanni Lombardo, G. A., Maksimovic-Ivanic, D., Mijatovic, S., Zocca, M., Perciavalle, V., Stosic-Grujicic, S., Nicoletti, F."Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells". Oncology Reports 28.2 (2012): 682-688.
Chicago
Donia, M., Mangano, K., Fagone, P., De Pasquale, R., Dinotta, F., Coco, M., Padron, J., Al-Abed, Y., Giovanni Lombardo, G. A., Maksimovic-Ivanic, D., Mijatovic, S., Zocca, M., Perciavalle, V., Stosic-Grujicic, S., Nicoletti, F."Unique antineoplastic profile of Saquinavir-NO, a novel NO-derivative of the protease inhibitor Saquinavir, on the in vitro and in vivo tumor formation of A375 human melanoma cells". Oncology Reports 28, no. 2 (2012): 682-688. https://doi.org/10.3892/or.2012.1840
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