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Article

Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis

  • Authors:
    • Shigehiro Osada
    • Shugo Suzuki
    • Chiaki Yoshimi
    • Miho Matsumoto
    • Tomoyuki Shirai
    • Satoru Takahashi
    • Masayoshi Imagawa
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Aichi 467‑8603, Japan, Department of Experimental Pathology and Tumor Biology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Aichi 467-8601, Japan
  • Pages: 1669-1674
    |
    Published online on: August 2, 2013
       https://doi.org/10.3892/or.2013.2651
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Abstract

Aberrant expression of regulators for epigenetics is involved in tumorigenesis. There is an urgent need to identify and characterize regulators concerned with epigenetics in the early stages of hepatocarcinogenesis. In the present study, we found that the expression of coactivator-associated arginine methyltransferase 1 (CARM1), a histone methyltransferase that functions as a cofactor for nuclear hormone receptors and several transcription factors, was elevated in adenomas and aberrant in carcinomas during hepatocellular carcinogenesis. In addition to RNA expression, immunohistochemical staining of liver sections revealed that CARM1 was highly expressed in the nucleus of tumor marker glutathione S-transferase placental form (GST-P)-positive foci. Neoplastic transformation of GST-P-positive foci guides the formation of hepatocellular carcinomas. CARM1 expression was not elevated in GST-P-negative regions. Furthermore, a luciferase reporter analysis revealed that CARM1 activated the Gst-p promoter in H4IIE, a hepatocellular carcinoma cell line. This activation was mediated by the enhancer element responsible for the carcinogenic-specific expression of Gst-p and nuclear factor E2-related factor 2. Knockdown of Carm1 by shRNA in H4IIE cells inhibited cell proliferation. These findings suggest that aberrantly expressed CARM1 in tumor marker-positive cells promotes tumorigenesis in the early stages of hepatocarcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Osada S, Suzuki S, Yoshimi C, Matsumoto M, Shirai T, Takahashi S and Imagawa M: Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis. Oncol Rep 30: 1669-1674, 2013.
APA
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., & Imagawa, M. (2013). Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis. Oncology Reports, 30, 1669-1674. https://doi.org/10.3892/or.2013.2651
MLA
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., Imagawa, M."Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis". Oncology Reports 30.4 (2013): 1669-1674.
Chicago
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., Imagawa, M."Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis". Oncology Reports 30, no. 4 (2013): 1669-1674. https://doi.org/10.3892/or.2013.2651
Copy and paste a formatted citation
x
Spandidos Publications style
Osada S, Suzuki S, Yoshimi C, Matsumoto M, Shirai T, Takahashi S and Imagawa M: Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis. Oncol Rep 30: 1669-1674, 2013.
APA
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., & Imagawa, M. (2013). Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis. Oncology Reports, 30, 1669-1674. https://doi.org/10.3892/or.2013.2651
MLA
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., Imagawa, M."Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis". Oncology Reports 30.4 (2013): 1669-1674.
Chicago
Osada, S., Suzuki, S., Yoshimi, C., Matsumoto, M., Shirai, T., Takahashi, S., Imagawa, M."Elevated expression of coactivator-associated arginine methyltransferase 1 is associated with early hepatocarcinogenesis". Oncology Reports 30, no. 4 (2013): 1669-1674. https://doi.org/10.3892/or.2013.2651
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