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Article

miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α

  • Authors:
    • Zhihao Wu
    • Xuehong Cai
    • Chenggang Huang
    • Jia Xu
    • An Liu
  • View Affiliations / Copyright

    Affiliations: The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 414000, P.R. China, The First People's Hospital of Yueyang, Yueyang, Hunan 325000, P.R. China
  • Pages: 1696-1702
    |
    Published online on: December 29, 2015
       https://doi.org/10.3892/or.2015.4529
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Abstract

Angiogenesis is a key factor in the growth and dissemination of malignant diseases, including breast cancer, with significant implications for its clinical management. It is known that microRNAs (miRNAs) play important roles in regulating tumor properties in cancers. However, whether miR-497 contributes to breast cancer angiogenesis remains unknown. Our study demonstrated that miR-497 was significantly downregulated in breast cancer tissue samples and cell lines. Conditioned medium obtained from breast cancer cell line MCF-7, treated with miR-497 mimics, suppressed the proliferation and tube formation of human umbilical vein endothelial cells in vitro, in comparison with the untransfected cells or cells transfected with the control vector alone. Furthermore, western blot assay confirmed that the overexpression of miR-497 reduced VEGF and HIF-1α protein levels. In addition, stable transfection of miR-497 inhibited tumorigenicity and angiogenesis in vivo. Moreover, HIF-1α was also increased in the breast cancer cells under a hypoxic condition, while the ectopic expression of miR-497 partially restored its level. Taken together, our findings indicate that miR-497 is a potential target for the biological therapy of breast cancer. Moreover, miR-497 inhibited the growth of tumors and reduced angiogenesis in a nude mouse xenograft tumor model, which was probably caused by the downregulation of pro-angiogenic molecules, such as VEGF and HIF-1α.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Schulze A and Harris AL: How cancer metabolism is tuned for proliferation and vulnerable to disruption. Nature. 491:364–373. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Parks SK, Chiche J and Pouysségur J: Disrupting proton dynamics and energy metabolism for cancer therapy. Nat Rev Cancer. 13:611–623. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Zheng R, Yao Q, Xie G, Du S, Ren C, Wang Y and Yuan Y: TAT-ODD-p53 enhances the radiosensitivity of hypoxic breast cancer cells by inhibiting Parkin-mediated mitophagy. Oncotarget. 6:17417–17429. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Hamdan FH and Zihlif MA: Gene expression alterations in chronic hypoxic MCF7 breast cancer cell line. Genomics. 104:477–481. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Soares RJ, Cagnin S, Chemello F, Silvestrin M, Musaro A, De Pitta C, Lanfranchi G and Sandri M: Involvement of microRNAs in the regulation of muscle wasting during catabolic conditions. J Biol Chem. 289:21909–21925. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Godnic I, Zorc M, Jevsinek Skok D, Calin GA, Horvat S, Dovc P, Kovac M and Kunej T: Genome-wide and species-wide in silico screening for intragenic MicroRNAs in human, mouse and chicken. PLoS One. 8:e651652013. View Article : Google Scholar : PubMed/NCBI

8 

Gromak N, Dienstbier M, Macias S, Plass M, Eyras E, Cáceres JF and Proudfoot NJ: Drosha regulates gene expression independently of RNA cleavage function. Cell Rep. 5:1499–1510. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Zhai H, Chen QJ, Chen BD, Yang YN, Ma YT, Li XM, Liu F, Yu ZX, Xiang Y, Liao W, et al: Long noncoding RNA MALAT1 as a putative biomarker of lymph node metastasis: A meta-analysis. Int J Clin Exp Med. 8:7648–7654. 2015.PubMed/NCBI

10 

Nagpal N, Ahmad HM, Chameettachal S, Sundar D, Ghosh S and Kulshreshtha R: HIF-inducible miR-191 promotes migration in breast cancer through complex regulation of TGFβ-signaling in hypoxic microenvironment. Sci Rep. 5:96502015. View Article : Google Scholar

11 

Chen W, Cai F, Zhang B, Barekati Z and Zhong XY: The level of circulating miRNA-10b and miRNA-373 in detecting lymph node metastasis of breast cancer: Potential biomarkers. Tumour Biol. 34:455–462. 2013. View Article : Google Scholar

12 

Du M, Shi D, Yuan L, Li P, Chu H, Qin C, Yin C, Zhang Z and Wang M: Circulating miR-497 and miR-663b in plasma are potential novel biomarkers for bladder cancer. Sci Rep. 5:104372015. View Article : Google Scholar : PubMed/NCBI

13 

Wei C, Luo Q, Sun X, Li D, Song H, Li X, Song J, Hua K and Fang L: microRNA-497 induces cell apoptosis by negatively regulating Bcl-2 protein expression at the posttranscriptional level in human breast cancer. Int J Clin Exp Pathol. 8:7729–7739. 2015.PubMed/NCBI

14 

Troppan K, Wenzl K, Pichler M, Pursche B, Schwarzenbacher D, Feichtinger J, Thallinger GG, Beham-Schmid C, Neumeister P and Deutsch A: miR-199a and miR-497 are associated with better overall survival due to increased chemosensitivity in diffuse large B-cell lymphoma patients. Int J Mol Sci. 16:18077–18095. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Han Z, Zhang Y, Yang Q, Liu B, Wu J, Zhang Y, Yang C and Jiang Y: miR-497 and miR-34a retard lung cancer growth by co-inhibiting cyclin E1 (CCNE1). Oncotarget. 6:13149–13163. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Peng L, Liu A, Shen Y, Xu HZ, Yang SZ, Ying XZ, Liao W, Liu HX, Lin ZQ, Chen QY, et al: Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway. Oncol Rep. 29:571–578. 2013.

17 

Howell A, Anderson AS, Clarke RB, Duffy SW, Evans DG, Garcia-Closas M, Gescher AJ, Key TJ, Saxton JM and Harvie MN: Risk determination and prevention of breast cancer. Breast Cancer Res. 16:4462014. View Article : Google Scholar : PubMed/NCBI

18 

Rinck A, Preusse M, Laggerbauer B, Lickert H, Engelhardt S and Theis FJ: The human transcriptome is enriched for miRNA-binding sites located in cooperativity-permitting distance. RNA Biol. 10:1125–1135. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Raychaudhuri S: MicroRNAs overexpressed in growth-restricted rat skeletal muscles regulate the glucose transport in cell culture targeting central TGF-β factor SMAD4. PLoS One. 7:e345962012. View Article : Google Scholar

20 

Luo Q, Li X, Gao Y, Long Y, Chen L, Huang Y and Fang L: MiRNA-497 regulates cell growth and invasion by targeting cyclin E1 in breast cancer. Cancer Cell Int. 13:952013. View Article : Google Scholar : PubMed/NCBI

21 

Liu Y, Wondimu A, Yan S, Bobb D and Ladisch S: Tumor gangliosides accelerate murine tumor angiogenesis. Angiogenesis. 17:563–571. 2014. View Article : Google Scholar :

22 

Retsky M, Bonadonna G, Demicheli R, Folkman J, Hrushesky W and Valagussa P: Hypothesis: Induced angiogenesis after surgery in premenopausal node-positive breast cancer patients is a major underlying reason why adjuvant chemotherapy works particularly well for those patients. Breast Cancer Res. 6:R372–R374. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Ma J, Zhang L, Ru GQ, Zhao ZS and Xu WJ: Upregulation of hypoxia inducible factor 1α mRNA is associated with elevated vascular endothelial growth factor expression and excessive angiogenesis and predicts a poor prognosis in gastric carcinoma. World J Gastroenterol. 13:1680–1686. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Dang K and Myers KA: The role of hypoxia-induced miR-210 in cancer progression. Int J Mol Sci. 16:6353–6372. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Meng F, Dong B, Li H, Fan D and Ding J: RNAi-mediated inhibition of Raf-1 leads to decreased angiogenesis and tumor growth in gastric cancer. Cancer Biol Ther. 8:174–179. 2009. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wu Z, Cai X, Huang C, Xu J and Liu A: miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncol Rep 35: 1696-1702, 2016.
APA
Wu, Z., Cai, X., Huang, C., Xu, J., & Liu, A. (2016). miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncology Reports, 35, 1696-1702. https://doi.org/10.3892/or.2015.4529
MLA
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35.3 (2016): 1696-1702.
Chicago
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35, no. 3 (2016): 1696-1702. https://doi.org/10.3892/or.2015.4529
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Z, Cai X, Huang C, Xu J and Liu A: miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncol Rep 35: 1696-1702, 2016.
APA
Wu, Z., Cai, X., Huang, C., Xu, J., & Liu, A. (2016). miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α. Oncology Reports, 35, 1696-1702. https://doi.org/10.3892/or.2015.4529
MLA
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35.3 (2016): 1696-1702.
Chicago
Wu, Z., Cai, X., Huang, C., Xu, J., Liu, A."miR-497 suppresses angiogenesis in breast carcinoma by targeting HIF-1α". Oncology Reports 35, no. 3 (2016): 1696-1702. https://doi.org/10.3892/or.2015.4529
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