Grifolin induces autophagic cell death by inhibiting the Akt/mTOR/S6K pathway in human ovarian cancer cells

  • Authors:
    • Xiaoxia Che
    • Hong Yan
    • Hengzi Sun
    • Samina Dongol
    • Yilin Wang
    • Qingtao Lv
    • Jie Jiang
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  • Published online on: May 30, 2016     https://doi.org/10.3892/or.2016.4840
  • Pages: 1041-1047
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Abstract

Grifolin, a secondary metabolic product isolated from the mushroom Albatrellus confluence, has been reported to possess antitumor activities in various tumors. To date, no report exists on the role of autophagy in grifolin-treated human ovarian cancer cells. In the present study, we investigated the effect and the mechanism of autophagy in ovarian cancer. Ovarian cancer cell lines A2780 and SKOV3 were treated with grifolin. Cell proliferation was assessed by MTT assay and the autophagic effect was determined using flow cytometry, electron microscopy, immunofluorescence staining and GFP-LC3 puncta formation assay. The expression of autophagy markers and the main autophagy-associated Akt/mTOR/S6K pathway proteins were measured by western blot analysis. MTT assay indicated that grifolin inhibits the proliferation of human ovarian cancer cell lines A2780 and SKOV3. Flow cytometry, electron microscopy, immunofluorescence and GFP-LC3 puncta formation assay proved that grifolin induces autophagic cell death in human ovarian cancer. The results of the western blot analysis suggested that grifolin treatment leads to upregulation of autophagy markers LC3B, Atg7, Beclin-1 along with downregulation of P62. In addition, the proteins of the pathways p-Akt, p-mTOR, p-p70S6K and p-4E-BP1 were downregulated while the total of these proteins remained unaffected. The present study indicated that grifolin could induce autophagic cell death in human ovarian cancer by inhibiting the Akt/mTOR/S6K pathway.
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August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Che X, Yan H, Sun H, Dongol S, Wang Y, Lv Q and Jiang J: Grifolin induces autophagic cell death by inhibiting the Akt/mTOR/S6K pathway in human ovarian cancer cells. Oncol Rep 36: 1041-1047, 2016
APA
Che, X., Yan, H., Sun, H., Dongol, S., Wang, Y., Lv, Q., & Jiang, J. (2016). Grifolin induces autophagic cell death by inhibiting the Akt/mTOR/S6K pathway in human ovarian cancer cells. Oncology Reports, 36, 1041-1047. https://doi.org/10.3892/or.2016.4840
MLA
Che, X., Yan, H., Sun, H., Dongol, S., Wang, Y., Lv, Q., Jiang, J."Grifolin induces autophagic cell death by inhibiting the Akt/mTOR/S6K pathway in human ovarian cancer cells". Oncology Reports 36.2 (2016): 1041-1047.
Chicago
Che, X., Yan, H., Sun, H., Dongol, S., Wang, Y., Lv, Q., Jiang, J."Grifolin induces autophagic cell death by inhibiting the Akt/mTOR/S6K pathway in human ovarian cancer cells". Oncology Reports 36, no. 2 (2016): 1041-1047. https://doi.org/10.3892/or.2016.4840