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Article

Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma

Corrigendum in: /10.3892/or.2020.7875
  • Authors:
    • Lu Fang
    • Meng Zhang
    • Lei Chen
    • Hu Xiong
    • Yukun Ge
    • Wei Lu
    • Xun Wu
    • Baoli Heng
    • Dexin Yu
    • Song Wu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Urology, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Urology, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Urology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, Department of Urology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518037, P.R. China
  • Pages: 1506-1516
    |
    Published online on: July 20, 2016
       https://doi.org/10.3892/or.2016.4955
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Abstract

Nucleolar and spindle-associated protein 1 (NUSAP1) is a microtubule-binding protein that plays an essential role in mitosis and cancer. Previous studies have demonstrated that NUSAP1 expression is relatively elevated in several malignancies. However, the biological roles of NUSAP1 in renal cell carcinoma (RCC) remain unknown. In the present study, we firstly performed reverse transcription‑polymerase chain reaction (RT-PCR) and western blot analysis to reveal that the expression of NUSAP1 was relatively elevated in clear cell RCC (ccRCC) tissue specimens and RCC cell lines. Immunohistochemical analysis showed that upregulation of NUSAP1 was significantly correlated with Fuhrman grade (P<0.001), tumor size (P=0.016), clinical stage (P<0.001) and distant metastasis (P=0.023). Additionally, high expression of NUSAP1 was closely associated with a shorter overall survival time of the ccRCC patients (P=0.006). Furthermore, we investigated the biological behaviors of RCC cells in vitro, and we identified that NUSAP1 depletion inhibited RCC cell migration, proliferation and invasion, and apoptosis was induced and the cell cycle was arrested. On the basis of our studies, NUSAP1 was identified as a potential prognostic indicator and a novel therapeutic target for RCC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Fang L, Zhang M, Chen L, Xiong H, Ge Y, Lu W, Wu X, Heng B, Yu D, Wu S, Wu S, et al: Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875. Oncol Rep 36: 1506-1516, 2016.
APA
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W. ... Wu, S. (2016). Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875. Oncology Reports, 36, 1506-1516. https://doi.org/10.3892/or.2016.4955
MLA
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W., Wu, X., Heng, B., Yu, D., Wu, S."Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875". Oncology Reports 36.3 (2016): 1506-1516.
Chicago
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W., Wu, X., Heng, B., Yu, D., Wu, S."Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875". Oncology Reports 36, no. 3 (2016): 1506-1516. https://doi.org/10.3892/or.2016.4955
Copy and paste a formatted citation
x
Spandidos Publications style
Fang L, Zhang M, Chen L, Xiong H, Ge Y, Lu W, Wu X, Heng B, Yu D, Wu S, Wu S, et al: Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875. Oncol Rep 36: 1506-1516, 2016.
APA
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W. ... Wu, S. (2016). Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875. Oncology Reports, 36, 1506-1516. https://doi.org/10.3892/or.2016.4955
MLA
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W., Wu, X., Heng, B., Yu, D., Wu, S."Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875". Oncology Reports 36.3 (2016): 1506-1516.
Chicago
Fang, L., Zhang, M., Chen, L., Xiong, H., Ge, Y., Lu, W., Wu, X., Heng, B., Yu, D., Wu, S."Downregulation of nucleolar and spindle-associated protein 1 expression suppresses cell migration, proliferation and invasion in renal cell carcinoma Corrigendum in /10.3892/or.2020.7875". Oncology Reports 36, no. 3 (2016): 1506-1516. https://doi.org/10.3892/or.2016.4955
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