Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib

  • Authors:
    • Yuezhao Huang
    • Kunyuan Wang
    • Chengxin Gu
    • Ganxiang Yu
    • Dan Zhao
    • Weijian Mai
    • Yun Zhong
    • Shiming Liu
    • Yuqiang Nie
    • Hui Yang
  • View Affiliations

  • Published online on: July 10, 2018     https://doi.org/10.3892/or.2018.6552
  • Pages: 1525-1532
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Sorafenib resistance is one of the major factors affecting the prognosis of patients with hepatocellular carcinoma (HCC). Increasing evidence has indicated that certain traditional medicines can enhance the sensitivity of cancer cells to sorafenib. Berberine, an isoquinoline alkaloid, has been demonstrated to possess antitumor properties against various malignancies. However, the synergistic effect of the combination of berberine and sorafenib in HCC remains unknown. The aim of the present study was to determine the effects of berberine and sorafenib combination on the growth of liver cancer cells. Initially, it was observed that the combination of sorafenib and berberine exerted a synergistic inhibitory effect on the proliferation of SMMC‑7721 and HepG2 cells in a dose‑ and time‑dependent manner by an MTS assay. Edu staining and colony formation assays also revealed that the combination of 100 µM berberine and 4 µM sorafenib exhibited a significant anti‑proliferation effect on SMMC‑7721 and HepG2 cells. Furthermore, western blotting assay indicated that the expressions levels of cleaved poly(ADP‑ribose) polymerase and cleaved caspase‑3 increased, while those of the anti‑apoptotic protein B‑cell lymphoma 2 and vascular endothelial growth factor decreased. To the best of our knowledge, this is the first study to demonstrate that berberine sensitized liver cancer cells to sorafenib treatment. These results suggest that berberine combined with sorafenib is able to inhibit the proliferation of liver cancer cells and induce apoptosis, which provides evidence for further clinical investigation in HCC patients with sorafenib resistance.

Related Articles

Journal Cover

September 2018
Volume 40 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Huang, Y., Wang, K., Gu, C., Yu, G., Zhao, D., Mai, W. ... Yang, H. (2018). Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib. Oncology Reports, 40, 1525-1532. https://doi.org/10.3892/or.2018.6552
MLA
Huang, Y., Wang, K., Gu, C., Yu, G., Zhao, D., Mai, W., Zhong, Y., Liu, S., Nie, Y., Yang, H."Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib". Oncology Reports 40.3 (2018): 1525-1532.
Chicago
Huang, Y., Wang, K., Gu, C., Yu, G., Zhao, D., Mai, W., Zhong, Y., Liu, S., Nie, Y., Yang, H."Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib". Oncology Reports 40, no. 3 (2018): 1525-1532. https://doi.org/10.3892/or.2018.6552