Open Access

Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression

  • Authors:
    • Ji‑Min Li
    • Guan‑Min Jiang
    • Liang Zhao
    • Fang Yang
    • Wei‑Qi Yuan
    • Hao Wang
    • Yi‑Qin Luo
  • View Affiliations

  • Published online on: August 22, 2019     https://doi.org/10.3892/or.2019.7291
  • Pages: 1725-1734
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxaliplatin (Oxa)‑based chemotherapy is widely used as the first‑line treatment for colorectal cancer (CRC). However, Oxa‑resistance is common for many postoperative CRC patients. To explore drug resistance in CRC, an Oxa‑resistant cell line, HCT116/Oxa, was established from parental HCT116 cells. These Oxa‑resistant cells exhibited characteristics of epithelial‑mesenchymal transition (EMT) and a higher migratory capacity than parental cells. Protein profiles of HCT116/Oxa and HCT116 cells were compared using a tandem mass tag‑based quantitative proteomics technique. The protein dehydrogenase/reductase SDR family member 2 (DHRS2) was revealed to be highly expressed in HCT116/Oxa cells. Silencing of DHRS2 in HCT116/Oxa cells effectively restored Oxa‑sensitivity by suppressing the expression of excision repair cross‑complementing group 1 protein via a p53‑dependent pathway, and reversed the EMT phenotype. Overall, the suppression of DHRS2 expression may be a promising strategy for the prevention of Oxa‑resistance in CRC.
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November-2019
Volume 42 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li JM, Jiang GM, Zhao L, Yang F, Yuan WQ, Wang H and Luo YQ: Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression. Oncol Rep 42: 1725-1734, 2019
APA
Li, J., Jiang, G., Zhao, L., Yang, F., Yuan, W., Wang, H., & Luo, Y. (2019). Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression. Oncology Reports, 42, 1725-1734. https://doi.org/10.3892/or.2019.7291
MLA
Li, J., Jiang, G., Zhao, L., Yang, F., Yuan, W., Wang, H., Luo, Y."Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression". Oncology Reports 42.5 (2019): 1725-1734.
Chicago
Li, J., Jiang, G., Zhao, L., Yang, F., Yuan, W., Wang, H., Luo, Y."Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression". Oncology Reports 42, no. 5 (2019): 1725-1734. https://doi.org/10.3892/or.2019.7291