Open Access

Knockdown of PNO1 inhibits esophageal cancer progression

  • Authors:
    • Guowen Wang
    • Qicai Li
    • Chuankui Li
    • Guixin Duan
    • Haiwei Sang
    • Haijun Dong
    • Yifan Yang
    • Chang Ma
    • Tao Tao
  • View Affiliations

  • Published online on: March 31, 2021     https://doi.org/10.3892/or.2021.8036
  • Article Number: 85
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the role of partner of NOB1 homolog (PNO1) in esophageal cancer (EC). The expression levels of PNO1 in EC were primarily analyzed using data obtained from databases. PNO1 expression was also knocked down in EC cells (Eca‑109 and TE1) to determine the biological effects of PNO1 on tumorigenesis in vitro and in vivo. In addition, possible downstream targets of PNO1 in EC were identified. The expression levels of PNO1 were upregulated in the tumor tissues compared with that noted in normal tissues. Moreover, the knockdown (KD) of PNO1 suppressed cell proliferation, migration and invasion, and promoted cell apoptosis (P<0.05). Furthermore, the protein expression levels of AKT1, Twist, Myc, mTOR, matrix metalloproteinase 2 (MMP2), nuclear factor (NF)‑κB p65 and β‑catenin 1 (CTNNB1) were downregulated following the KD of PNO1 in Eca‑109 cells (P<0.05). In addition, the overexpression of CTNNB1 reversed the effects of PNO1 KD in Eca‑109 cells (P<0.05). In conclusion, the findings of the present study suggest that PNO1 promotes EC progression by regulating AKT1, Twist, Myc, mTOR, MMP2, NF‑κB p65 and CTNNB1 expression.
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May-2021
Volume 45 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Wang G, Li Q, Li C, Duan G, Sang H, Dong H, Yang Y, Ma C and Tao T: Knockdown of PNO1 inhibits esophageal cancer progression. Oncol Rep 45: 85, 2021
APA
Wang, G., Li, Q., Li, C., Duan, G., Sang, H., Dong, H. ... Tao, T. (2021). Knockdown of PNO1 inhibits esophageal cancer progression. Oncology Reports, 45, 85. https://doi.org/10.3892/or.2021.8036
MLA
Wang, G., Li, Q., Li, C., Duan, G., Sang, H., Dong, H., Yang, Y., Ma, C., Tao, T."Knockdown of PNO1 inhibits esophageal cancer progression". Oncology Reports 45.5 (2021): 85.
Chicago
Wang, G., Li, Q., Li, C., Duan, G., Sang, H., Dong, H., Yang, Y., Ma, C., Tao, T."Knockdown of PNO1 inhibits esophageal cancer progression". Oncology Reports 45, no. 5 (2021): 85. https://doi.org/10.3892/or.2021.8036