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Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma

  • Authors:
    • Xin Zuo
    • Weiling Li
    • Xiaofang Yan
    • Tieliang Ma
    • Yan Ren
    • Meijuan Hua
    • Huiyun Yang
    • Haifeng Wu
    • Hongdi Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China
    Copyright: © Zuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 186
    |
    Published online on: July 9, 2021
       https://doi.org/10.3892/or.2021.8137
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Abstract

Endometrial carcinoma (EC) is the most common cancer in women worldwide, yet little is known about the underlying molecular basis of EC development. LINC01224, a novel long non‑coding (lnc)RNA, was recently identified as an oncogene in various types of cancer. However, the function and underlying mechanism of LINC01224 in EC is still unclear. A total of 50 pairs of tumor and adjacent normal tissue from patients with EC, three EC cell lines and one human normal endometrial stromal cell (ESC) line were subjected to reverse transcription‑quantitative PCR assay to evaluate the expression levels of LINC01224. Cell Counting Kit‑8, colony formation and flow cytometry assays were used to assess cell proliferation and apoptosis. Western blotting was used to measure expression levels of apoptosis‑ and proliferation‑associated proteins and AKT3 protein. A xenograft model of HEC1A cells was established to validate the in vivo function of LINC01224 in EC tumor growth. Starbase 3.0 database prediction and luciferase reporter and RNA pull‑down assays were performed to verify the binding sites between LINC01224 and microRNA (miR)‑485‑5p and miR‑485‑5p and AKT3. LINC01224 expression was significantly upregulated in both EC tumor tissue and cell lines. The upregulation of LINC01224 was negatively associated with survival of patients with EC. Functionally, LINC01224 promoted proliferation and inhibited apoptosis of EC cells; LINC01224 directly bound to and downregulated miR‑485‑5p to elevate the expression levels of AKT3, thereby promoting EC progression. LINC01224 depletion in EC cells hindered tumor growth in a xenograft model. The tumor suppressing effect of LINC01224‑knockdown on EC progression was partly rescued by treatment with miR‑485‑5p inhibitor. The present data demonstrated the expression levels, clinical relevance and functional mechanism of LINC01224 in EC. LINC01224 promoted EC development via sponging miR‑485‑5p to elevate AKT3 expression levels; this may provide a promising therapeutic target pathway for EC treatment.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

D'Andrilli G, Bovicelli A, Paggi MG and Giordano A: New insights in endometrial carcinogenesis. J Cell Physiol. 227:2842–2846. 2012. View Article : Google Scholar

3 

Buhtoiarova TN, Brenner CA and Singh M: Endometrial carcinoma: Role of current and emerging biomarkers in resolving persistent clinical dilemmas. Am J Clin Pathol. 145:8–21. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Lorenzi L, Avila Cobos F, Decock A, Everaert C, Helsmoortel H, Lefever S, Verboom K, Volders PJ, Speleman F, Vandesompele J and Mestdagh P: Long noncoding RNA expression profiling in cancer: Challenges and opportunities. Genes Chromosomes Cancer. 58:191–199. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Derrien T, Johnson R, Bussotti G, Tanzer A, Djebali S, Tilgner H, Guernec G, Martin D, Merkel A, Knowles DG, et al: The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression. Genome Res. 22:1775–1789. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Geisler S and Coller J: RNA in unexpected places: Long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 14:699–712. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Khalil AM, Guttman M, Huarte M, Garber M, Raj A, Rivea Morales D, Thomas K, Presser A, Bernstein BE, van Oudenaarden A, et al: Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression. Proc Natl Acad Sci USA. 106:11667–11672. 2009. View Article : Google Scholar : PubMed/NCBI

8 

DiStefano JK: The emerging role of long noncoding RNAs in human disease. Disease Gene Identification: Methods and Protocols. DiStefano JK: Springer; New York, NY: pp. 91–110. 2018, View Article : Google Scholar

9 

Hu G, Niu F, Humburg BA, Liao K, Bendi S, Callen S, Fox HS and Buch S: Molecular mechanisms of long noncoding RNAs and their role in disease pathogenesis. Oncotarget. 9:18648–18663. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Kondo Y, Shinjo K and Katsushima K: Long non-coding RNAs as an epigenetic regulator in human cancers. Cancer Sci. 108:1927–1933. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Haemmerle M and Gutschner T: Long non-coding RNAs in cancer and development: Where do we go from here? Int J Mol Sci. 16:1395–1405. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Gong D, Feng PC, Ke XF, Kuang HL, Pan LL, Ye Q and Wu JB: Silencing long non-coding RNA LINC01224 inhibits hepatocellular carcinoma progression via microRNA-330-5p-induced inhibition of CHEK1. Mol Ther Nucleic Acids. 19:482–497. 2020. View Article : Google Scholar : PubMed/NCBI

13 

Xing S, Zhang Y and Zhang J: LINC01224 exhibits cancer-promoting activity in epithelial ovarian cancer through microRNA-485-5p-mediated PAK4 upregulation. Onco Targets Ther. 13:5643–5655. 2020. View Article : Google Scholar : PubMed/NCBI

14 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Mishra S, Yadav T and Rani V: Exploring miRNA based approaches in cancer diagnostics and therapeutics. Crit Rev Oncol Hematol. 98:12–23. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Hansen TB, Jensen TI, Clausen BH, Bramsen JB, Finsen B, Damgaard CK and Kjems J: Natural RNA circles function as efficient microRNA sponges. Nature. 495:384–388. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Smith CJ and Osborn AM: Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 67:6–20. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Pritt SL and Smith TM: Institutional animal care and use committee postapproval monitoring programs: A proposed comprehensive classification scheme. J Am Assoc Lab Anim Sci. 59:127–131. 2020. View Article : Google Scholar : PubMed/NCBI

19 

Li L and Ma L: Upregulation of miR-582-5p regulates cell proliferation and apoptosis by targeting AKT3 in human endometrial carcinoma. Saudi J Biol Sci. 25:965–970. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Fang Y, Liang X, Xu J and Cai X: miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer. Cancer Manag Res. 10:6537–6547. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Wang Y, Hou J, He D, Sun M, Zhang P, Yu Y and Chen Y: The emerging function and mechanism of ceRNAs in cancer. Trends Genet. 32:211–224. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Wang KC and Chang HY: Molecular mechanisms of long noncoding RNAs. Mol Cell. 43:904–914. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Adams BD, Kasinski AL and Slack FJ: Aberrant regulation and function of microRNAs in cancer. Curr Biol. 24:R762–R776. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Bao W, Cao F, Ni S, Yang J, Li H, Su Z and Zhao B: lncRNA FLVCR1-AS1 regulates cell proliferation, migration and invasion by sponging miR-485-5p in human cholangiocarcinoma. Oncol Lett. 18:2240–2247. 2019.PubMed/NCBI

25 

Tu J, Zhao Z, Xu M, Chen M, Weng Q and Ji J: LINC00460 promotes hepatocellular carcinoma development through sponging miR-485-5p to up-regulate PAK1. Biomed Pharmacother. 118:1092132019. View Article : Google Scholar : PubMed/NCBI

26 

Peng Y, Leng W, Duan S and Hong M: Long noncoding RNA BLACAT1 is overexpressed in hepatocellular carcinoma and its downregulation suppressed cancer cell development through endogenously competing against hsa-miR-485-5p. Biomed Pharmacother. 116:1090272019. View Article : Google Scholar : PubMed/NCBI

27 

Lu H, Wang C, Xue L, Zhang Q, Luh F, Wang J, Lin TG, Yen Y and Liu X: Human mitotic centromere-associated kinesin is targeted by MicroRNA 485-5p/181c and prognosticates poor survivability of breast cancer. J Oncol. 2019:23162372019. View Article : Google Scholar : PubMed/NCBI

28 

Gao F, Wu H, Wang R, Guo Y, Zhang Z, Wang T, Zhang G, Liu C and Liu J: MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2. Bioengineered. 10:1–12. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Shao Y and Aplin AE: Akt3-mediated resistance to apoptosis in B-RAF-targeted melanoma cells. Cancer Res. 70:6670–6681. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zuo X, Li W, Yan X, Ma T, Ren Y, Hua M, Yang H, Wu H and Zhu H: Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma. Oncol Rep 46: 186, 2021.
APA
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M. ... Zhu, H. (2021). Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma. Oncology Reports, 46, 186. https://doi.org/10.3892/or.2021.8137
MLA
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M., Yang, H., Wu, H., Zhu, H."Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma". Oncology Reports 46.3 (2021): 186.
Chicago
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M., Yang, H., Wu, H., Zhu, H."Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma". Oncology Reports 46, no. 3 (2021): 186. https://doi.org/10.3892/or.2021.8137
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo X, Li W, Yan X, Ma T, Ren Y, Hua M, Yang H, Wu H and Zhu H: Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma. Oncol Rep 46: 186, 2021.
APA
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M. ... Zhu, H. (2021). Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma. Oncology Reports, 46, 186. https://doi.org/10.3892/or.2021.8137
MLA
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M., Yang, H., Wu, H., Zhu, H."Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma". Oncology Reports 46.3 (2021): 186.
Chicago
Zuo, X., Li, W., Yan, X., Ma, T., Ren, Y., Hua, M., Yang, H., Wu, H., Zhu, H."Long non‑coding RNA LINC01224 promotes cell proliferation and inhibits apoptosis by regulating AKT3 expression via targeting miR‑485‑5p in endometrial carcinoma". Oncology Reports 46, no. 3 (2021): 186. https://doi.org/10.3892/or.2021.8137
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