Comparison of the multigene panel test and OncoScan™ for the determination of HER2 amplification in breast cancer

  • Authors:
    • Kaori Akazawa
    • Naofumi Kagara
    • Yoshiaki Sota
    • Daisuke Motooka
    • Shota Nakamura
    • Tomohiro Miyake
    • Tomonori Tanei
    • Yasuto Naoi
    • Masafumi Shimoda
    • Seung Jin Kim
    • Shinzaburo Noguchi
    • Kenzo Shimazu
  • View Affiliations

  • Published online on: August 10, 2021     https://doi.org/10.3892/or.2021.8168
  • Article Number: 217
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Abstract

The diagnostic accuracy of the multigene panel test (MPT) and OncoScan™ in the determination of HER2 amplification in breast tumors remains controversial. In the present study, HER2 copy number was analyzed using both MPT and OncoScan™ in 45 breast tumors and was compared with that in fluorescent in situ hybridization (FISH) analysis. Tumors with low cellularity were examined using tumor cell enrichment and fluorescence‑activated cell sorting. Both MPT and OncoScan™ exhibited significant correlations with FISH with respect to the determination of HER2 amplification in breast tumors. However, the correlation coefficient was significantly higher for the comparison of MPT and FISH (r=0.770) compared with that between OncoScan™ and FISH (r=0.564). The accuracy of MPT (93.3%) was slightly higher compared with that in OncoScan™ (84.4%) in determining the HER2 status, which was mostly explained by the higher sensitivity of MPT in tumors with low cellularity (83.3 vs. 33.3%), but not in those with high cellularity (81.8 vs. 72.7%). The specificity was 100% for both tests. The MPT exhibited higher sensitivity in the determination of the amplification of other genes, including MYC, fibroblast growth factor receptor 1 and GATA binding protein 3 in tumors with low cellularity compared with that in tumors with high cellularity. OncoScan™ exhibited low sensitivity without tumor cell enrichment. The results suggested that MPT could be a promising method to determine HER2 status in breast tumors and that it could exhibit improved accuracy compared with that in OncoScan™ in tumors with low cellularity.
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October-2021
Volume 46 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Akazawa K, Kagara N, Sota Y, Motooka D, Nakamura S, Miyake T, Tanei T, Naoi Y, Shimoda M, Kim SJ, Kim SJ, et al: Comparison of the multigene panel test and OncoScan™ for the determination of <em>HER2</em> amplification in breast cancer. Oncol Rep 46: 217, 2021
APA
Akazawa, K., Kagara, N., Sota, Y., Motooka, D., Nakamura, S., Miyake, T. ... Shimazu, K. (2021). Comparison of the multigene panel test and OncoScan™ for the determination of <em>HER2</em> amplification in breast cancer. Oncology Reports, 46, 217. https://doi.org/10.3892/or.2021.8168
MLA
Akazawa, K., Kagara, N., Sota, Y., Motooka, D., Nakamura, S., Miyake, T., Tanei, T., Naoi, Y., Shimoda, M., Kim, S. J., Noguchi, S., Shimazu, K."Comparison of the multigene panel test and OncoScan™ for the determination of <em>HER2</em> amplification in breast cancer". Oncology Reports 46.4 (2021): 217.
Chicago
Akazawa, K., Kagara, N., Sota, Y., Motooka, D., Nakamura, S., Miyake, T., Tanei, T., Naoi, Y., Shimoda, M., Kim, S. J., Noguchi, S., Shimazu, K."Comparison of the multigene panel test and OncoScan™ for the determination of <em>HER2</em> amplification in breast cancer". Oncology Reports 46, no. 4 (2021): 217. https://doi.org/10.3892/or.2021.8168