Open Access

Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway

  • Authors:
    • Hualing Li
    • Bei Lv
    • Ling Kong
    • Jing Xia
    • Ming Zhu
    • Lijuan Hu
    • Danyang Zhen
    • Yifan Wu
    • Xiaoqin Jia
    • Sujuan Zhu
    • Hengmi Cui
  • View Affiliations

  • Published online on: August 3, 2017     https://doi.org/10.3892/ijmm.2017.3089
  • Pages: 1125-1133
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neuro-oncological ventral antigen 1 (Nova1) is a well known brain-specific splicing factor. Several studies have identified Nova1 as a regulatory protein at the top of a hierarchical network. However, the function of Nova1 during hypoxia remains poorly understood. This study aimed to investigate the protective effect of Nova1 against cell hypoxia and to further explore the Bax/Bcl-2/caspase-3 pathway as a potential mechanism. During hypoxia, the survival rate of pheochromocytoma PC12 cells was gradually decreased and the apoptosis rate was gradually increased, peaking at 48 h of hypoxia. At 48 h after transfection of PC12 cells with pCMV-Myc-Nova1, the expression of Nova1 was significantly increased, with wide distribution in the cytoplasm and nucleus. Moreover, the survival rate was significantly increased and the apoptosis rate was significantly decreased. Additionally, the mRNA and protein expression levels of Bax and caspase-3 were significantly increased in the pCMV-Myc group and significantly decreased in the pCMV‑Myc-Nova1 group, whereas that of Bcl-2 was significantly decreased in the pCMV-Myc group and significantly increased in the pCMV-Myc-Nova1 group. This study indicated that Nova1 could be linked to resistance to the hypoxia-induced apoptosis of PC12 cells via the Bax/Bcl-2/caspase-3 pathway, and this finding may be of significance for exploring novel mechanisms of hypoxia and the treatment of hypoxia-associated diseases.
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October-2017
Volume 40 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li H, Lv B, Kong L, Xia J, Zhu M, Hu L, Zhen D, Wu Y, Jia X, Zhu S, Zhu S, et al: Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway. Int J Mol Med 40: 1125-1133, 2017
APA
Li, H., Lv, B., Kong, L., Xia, J., Zhu, M., Hu, L. ... Cui, H. (2017). Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway. International Journal of Molecular Medicine, 40, 1125-1133. https://doi.org/10.3892/ijmm.2017.3089
MLA
Li, H., Lv, B., Kong, L., Xia, J., Zhu, M., Hu, L., Zhen, D., Wu, Y., Jia, X., Zhu, S., Cui, H."Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway". International Journal of Molecular Medicine 40.4 (2017): 1125-1133.
Chicago
Li, H., Lv, B., Kong, L., Xia, J., Zhu, M., Hu, L., Zhen, D., Wu, Y., Jia, X., Zhu, S., Cui, H."Nova1 mediates resistance of rat pheochromocytoma cells to hypoxia-induced apoptosis via the Bax/Bcl-2/caspase-3 pathway". International Journal of Molecular Medicine 40, no. 4 (2017): 1125-1133. https://doi.org/10.3892/ijmm.2017.3089