Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway

  • Authors:
    • Liang Wang
    • Ting Zhang
    • Yongyong Xi
    • Cuili Yang
    • Chengcao Sun
    • Dejia Li
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  • Published online on: May 27, 2016     https://doi.org/10.3892/mmr.2016.5346
  • Pages: 1309-1315
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Abstract

Accumulating evidence suggests that Sirtuin (Sirt)1 serves a significant role in proliferation and differentiation of myoblast cells; however the signaling mechanisms involved remain to be established. Myostatin (MSTN), a member of transforming growth factor‑β family, is an vital regulator of myoblast, fibroblast growth and differentiation. To determine if MSTN is involved in the regulation of myoblast cell proliferation by Sirt1, the present study administrated the Sirt1 activator resveratrol, inhibitor nicotinamide (NAM) and MSTN inhibitor SB431542 to C2C12 myoblast cells. It was demonstrated that the Sirt1 activator, resveratrol, repressed, whereas the Sirt1 inhibitor, NAM, enhanced C2C12 myoblast cells proliferation in a Sirt1‑dependent manner. SB431542 promoted the proliferation of C2C12 myoblast cells and reversed the inhibition effect of NAM on C2C12 myoblast cell proliferation. Additionally, resveratrol upregulated the mRNA expression of MyoD, but inhibited the expression of MSTN. Additionally, NAM significantly repressed the expression of MyoD and the phosphorylation of P107 (p‑P107), but enhanced the expression of MSTN and the protein expression of P107. SB431542 significantly mitigated the effect of NAM on the expression of MyoD, P107 and p‑P107. Taken together, these results indicated that Sirt1 promotes the proliferation of C2C12 myoblast cells via the MSTN signaling pathway.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang L, Zhang T, Xi Y, Yang C, Sun C and Li D: Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway. Mol Med Rep 14: 1309-1315, 2016.
APA
Wang, L., Zhang, T., Xi, Y., Yang, C., Sun, C., & Li, D. (2016). Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway. Molecular Medicine Reports, 14, 1309-1315. https://doi.org/10.3892/mmr.2016.5346
MLA
Wang, L., Zhang, T., Xi, Y., Yang, C., Sun, C., Li, D."Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway". Molecular Medicine Reports 14.2 (2016): 1309-1315.
Chicago
Wang, L., Zhang, T., Xi, Y., Yang, C., Sun, C., Li, D."Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway". Molecular Medicine Reports 14, no. 2 (2016): 1309-1315. https://doi.org/10.3892/mmr.2016.5346