Long intergenic noncoding RNA p21 mediates oxidized LDL‑induced apoptosis and expression of LOX‑1 in human coronary artery endothelial cells

  • Authors:
    • Tao Zhou
    • Xiaofang Chen
  • View Affiliations

  • Published online on: September 26, 2017     https://doi.org/10.3892/mmr.2017.7623
  • Pages:8513-8519
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Abstract

Atherosclerosis is the most common pathological cause of cardiovascular diseases, and endothelial dysfunction has a vital role. It has been suggested that inhibiting endothelial cell apoptosis induced by oxidized low‑density lipoprotein (ox‑LDL), an essential atherosclerotic factor, is a potential novel therapeutic strategy against atherosclerosis. Previous studies have revealed that endothelial lectin‑like ox‑LDL receptor‑1 (LOX‑1) and long intergenic noncoding RNA p21 (lincRNA‑p21) may serve as therapeutic targets for atherosclerosis and associated cardiovascular disorders. The present study investigated the role of lincRNA‑p21 in oxLDL‑induced apoptosis and expression of LOX‑1 in human coronary artery endothelial cells (HCAECs). Primary HCAECs were treated with ox‑LDL (30, 60 or 90 µg/ml) for 24 or 48 h, and the expression of lincRNA‑p21, LOX‑1 and cell apoptosis rate were measured. Ox‑LDL dose‑ and time‑dependently induced the expression of lincRNA‑p21 and LOX‑1 and apoptosis in HCAECs. Lentiviral overexpression of lincRNA‑p21 markedly increased oxLDL‑induced apoptosis and the expression of LOX‑1 in HCAECs. Additionally, the effect was largely blocked by selective protein kinase C (PKC) inhibitor, rottlerin. However, lentiviral knockdown of lincRNA‑p21 markedly decreased oxLDL‑induced apoptosis and the expression of LOX‑1. In addition, overexpression and knockdown of lincRNA‑p21 markedly increased and decreased oxLDL‑induced PKCδ activity/phosphorylation, respectively. In conclusion, to the best of our knowledge, the present study provides the first evidence indicating that lincRNA‑p21 is a major mediator of oxLDL‑induced apoptosis and expression of LOX‑1 in human vascular endothelial cells, and acts via activation of PKCδ. These results provide insights into the role of lincRNA‑p21 in the pathogenesis of atherosclerosis.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Zhou, T., & Zhou, T. (2017). Long intergenic noncoding RNA p21 mediates oxidized LDL‑induced apoptosis and expression of LOX‑1 in human coronary artery endothelial cells. Molecular Medicine Reports, 16, 8513-8519. https://doi.org/10.3892/mmr.2017.7623
MLA
Zhou, T., Chen, X."Long intergenic noncoding RNA p21 mediates oxidized LDL‑induced apoptosis and expression of LOX‑1 in human coronary artery endothelial cells". Molecular Medicine Reports 16.6 (2017): 8513-8519.
Chicago
Zhou, T., Chen, X."Long intergenic noncoding RNA p21 mediates oxidized LDL‑induced apoptosis and expression of LOX‑1 in human coronary artery endothelial cells". Molecular Medicine Reports 16, no. 6 (2017): 8513-8519. https://doi.org/10.3892/mmr.2017.7623