Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts

  • Authors:
    • Guoquan Li
    • Bingbing Xie
    • Xiaolong Li
    • Yinghai Chen
    • Yinghui Xu
    • Meng Xu-Welliver
    • Lijuan Zou
  • View Affiliations

  • Published online on: January 19, 2015     https://doi.org/10.3892/or.2015.3732
  • Pages: 1427-1433
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Abstract

β-elemene, the active component of elemene (1-methyl-1-vinyl-2,4-diisopropenyl-cyclohexane), is a naturally occurring compound isolated from the traditional Chinese medicinal herb Curcuma wenyujin. Studies have confirmed that β-elemene enhances the radiosensitivity of lung cancer cell lines such as A549, by multiple pathways; however, their underlying mechanisms and pathways are yet to be elucidated. In the present study, two-dimensional differential in-gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry were used to profile the different proteins in A549 cell xenograft models of both treatment groups. The protein/mRNA expression was assessed by reverse transcription-polymerase chain reaction and western blotting techniques in tumor samples from all treatment groups. As a critical player in redox regulation of cancer cells, inhibition of peroxiredoxin-1 (Prx-1) may be an effective option for enhancing the tumor response to radiation. We further verified Prx-1 expression at the transcription and translation levels. β-elemene at a dose of 45 mg/kg had little effect on the Prx-1 protein expression, which was correlated with a moderate antitumor effect. However, a 45 mg/kg dose of β-elemene significantly inhibited the Prx-1 mRNA expression, thereby suggesting a possible influence on the transcriptional process, and radiation significantly increased the Prx-1 mRNA/protein expression compared to the control group (p<0.01). Notably, Prx-1 mRNA/protein expression was significantly lower in the β-elemene/radiation co-treatment group compared to the baseline levels in the control group (p<0.01). These results suggest that radiation-induced Prx-1 expression is directly or indirectly suppressed by β-elemene, thus suggesting a new pathway by which to reverse radioresistance.

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March 2015
Volume 33 Issue 3

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Online ISSN:1791-2431

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Copy and paste a formatted citation
APA
Li, G., Xie, B., Li, X., Chen, Y., Xu, Y., Xu-Welliver, M., & Zou, L. (2015). Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts. Oncology Reports, 33, 1427-1433. https://doi.org/10.3892/or.2015.3732
MLA
Li, G., Xie, B., Li, X., Chen, Y., Xu, Y., Xu-Welliver, M., Zou, L."Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts". Oncology Reports 33.3 (2015): 1427-1433.
Chicago
Li, G., Xie, B., Li, X., Chen, Y., Xu, Y., Xu-Welliver, M., Zou, L."Downregulation of peroxiredoxin-1 by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenografts". Oncology Reports 33, no. 3 (2015): 1427-1433. https://doi.org/10.3892/or.2015.3732