miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer

  • Authors:
    • Hui Fu
    • Lei Fu
    • Chao Xie
    • Wen-Shu Zuo
    • Yan-Song Liu
    • Mei-Zhu Zheng
    • Jin-Ming Yu
  • View Affiliations

  • Published online on: January 9, 2017     https://doi.org/10.3892/or.2017.5360
  • Pages: 1093-1099
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Abstract

Cancer stem cell (CSC) formation and epithelial-mesenchymal transition (EMT) are pivotal events in tumor cell invasion and metastasis. They have been shown to occur in resistance to tamoxifen. Moreover, microRNAs (miRNAs) have been associated with CSCs, EMT as well as tamoxifen resistance. Studying molecular mechanism of CSCs, EMT as well as tamoxifen resistance will help us to further understand the pathogenesis and progression of the disease and offer new targets for effective therapies. In the present study, we showed that miR-375 inhibits CSC traits in breast cancer MCF-7 cells. Bioinformatics analysis and experimental validation identified HOXB3 as a direct target of miR-375. Overexpressing miR-375 degraded HOXB3 mRNA in MCF-7 cells. Moreover, overexpression of HOXB3 induced formation of CSC phenotypes, EMT and tamoxifen-resistance as well as enhanced ability of migration and invasion in MCF-7 cells. Most ER-positive breast cancer-related deaths occur, because of resistance to standard therapies and metastasis, restoring miR-375 or targeting HOXB3 might serve as potential therapeutic approaches for the treatment of tamoxifen-resistant breast cancer.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fu H, Fu L, Xie C, Zuo W, Liu Y, Zheng M and Yu J: miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer. Oncol Rep 37: 1093-1099, 2017.
APA
Fu, H., Fu, L., Xie, C., Zuo, W., Liu, Y., Zheng, M., & Yu, J. (2017). miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer. Oncology Reports, 37, 1093-1099. https://doi.org/10.3892/or.2017.5360
MLA
Fu, H., Fu, L., Xie, C., Zuo, W., Liu, Y., Zheng, M., Yu, J."miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer". Oncology Reports 37.2 (2017): 1093-1099.
Chicago
Fu, H., Fu, L., Xie, C., Zuo, W., Liu, Y., Zheng, M., Yu, J."miR-375 inhibits cancer stem cell phenotype and tamoxifen resistance by degrading HOXB3 in human ER-positive breast cancer". Oncology Reports 37, no. 2 (2017): 1093-1099. https://doi.org/10.3892/or.2017.5360