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Article Open Access

Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis

  • Authors:
    • Lanqi Li
    • Donghong Cai
    • Huiya Zhong
    • Fengbin Liu
    • Qilong Jiang
    • Jian Liang
    • Peiwu Li
    • Yafang Song
    • Aidong Ji
    • Wei Jiao
    • Jingwei Song
    • Jinqiu Li
    • Zhiwei Chen
    • Qing Li
    • Lingling Ke
  • View Affiliations / Copyright

    Affiliations: Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Gastrosplenic Diseases, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 307
    |
    Published online on: February 24, 2022
       https://doi.org/10.3892/etm.2022.11236
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Abstract

Due to challenges in diagnosing myasthenia gravis (MG), identifying novel diagnostic biomarkers for this disease is essential. Mitochondria are key organelles that regulate multiple physiological functions, such as energy production, cell proliferation and cell death. In the present study, Mfn1/2, Opa1, Drp1, Fis1, AMPK, PGC‑1α, NRF‑1 and TFAM were compared between patients with MG and healthy subjects to identify potential diagnostic biomarkers for MG. Blood samples were collected from 50 patients with MG and 50 healthy subjects. The participants' demographic information and routine blood test results were recorded. Mitochondrial dynamics were evaluated and levels of Mfn1/2, Opa1, Drp1, Fis1, AMPK, PGC‑1α, NRF‑1 and TFAM were determined in peripheral blood mononuclear cells using western blotting and reverse transcription‑quantitative PCR, respectively. Receiver operating characteristic curve analysis was used to evaluate the diagnostic accuracy of these indicators. The areas under the curve values of Mfn1/2, Opa1, Drp1, Fis1,AMPK, PGC‑1α, NRF‑1 and TFAM were 0.5408‑0.8696. Compared with control subjects, mRNA expression levels of Mfn1/2, Opa1, AMPK, PGC‑1α, NRF‑1 and TFAM were lower, while those of Drp1 and Fis1 were higher in patients with MG. The protein expression levels of all these molecules were lower in patients with MG than in control subjects. These results suggested that mitochondrial dynamics and biogenesis indicators may be diagnostic biomarkers for MG.
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Li L, Cai D, Zhong H, Liu F, Jiang Q, Liang J, Li P, Song Y, Ji A, Jiao W, Jiao W, et al: Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Exp Ther Med 23: 307, 2022.
APA
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J. ... Ke, L. (2022). Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Experimental and Therapeutic Medicine, 23, 307. https://doi.org/10.3892/etm.2022.11236
MLA
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J., Li, P., Song, Y., Ji, A., Jiao, W., Song, J., Li, J., Chen, Z., Li, Q., Ke, L."Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis". Experimental and Therapeutic Medicine 23.4 (2022): 307.
Chicago
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J., Li, P., Song, Y., Ji, A., Jiao, W., Song, J., Li, J., Chen, Z., Li, Q., Ke, L."Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis". Experimental and Therapeutic Medicine 23, no. 4 (2022): 307. https://doi.org/10.3892/etm.2022.11236
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Spandidos Publications style
Li L, Cai D, Zhong H, Liu F, Jiang Q, Liang J, Li P, Song Y, Ji A, Jiao W, Jiao W, et al: Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Exp Ther Med 23: 307, 2022.
APA
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J. ... Ke, L. (2022). Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Experimental and Therapeutic Medicine, 23, 307. https://doi.org/10.3892/etm.2022.11236
MLA
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J., Li, P., Song, Y., Ji, A., Jiao, W., Song, J., Li, J., Chen, Z., Li, Q., Ke, L."Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis". Experimental and Therapeutic Medicine 23.4 (2022): 307.
Chicago
Li, L., Cai, D., Zhong, H., Liu, F., Jiang, Q., Liang, J., Li, P., Song, Y., Ji, A., Jiao, W., Song, J., Li, J., Chen, Z., Li, Q., Ke, L."Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis". Experimental and Therapeutic Medicine 23, no. 4 (2022): 307. https://doi.org/10.3892/etm.2022.11236
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