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Article

IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression

  • Authors:
    • Chenhui Zhao
    • Yongting Li
    • Weiming Zhang
    • Dan Zhao
    • Ling Ma
    • Pei Ma
    • Fengming Yang
    • Yingwei Wang
    • Yongqian Shu
    • Wen Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China, Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 1579-1592
    |
    Published online on: March 7, 2018
       https://doi.org/10.3892/ijo.2018.4307
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Abstract

Non-small cell lung cancer (NSCLC) is considered to be an inflammation-associated carcinoma. Although interleukin‑17 (IL‑17) production contributes to the proliferation and growth of NSCLC, the mechanisms underlying IL‑17-induced NSCLC cell proliferation have not been fully elucidated. In the present study, by using ELISA and immunohistochemical analyses, we first found that the expression levels of IL‑17, IL‑17 receptor (IL‑17R), high-mobility group A1 (HMGA1) and cyclin D1 were elevated in the samples of patients with NSCLC. Subsequently, by RT-qPCR, western blot analysis and cell proliferation assay in vitro, we revealed that stimulation with recombinant human IL‑17 (namely IL‑17A) markedly induced the expression of HMGA1 and cyclin D1 in the A549 cells (a human lung adenocarcinoma cell line) and promoted cell proliferation. Furthermore, luciferase reporter and ChIP assays confirmed that upregulated HMGA1 directly bound to the cyclin D1 gene promoter and activated its transcription. Notably, the response element of HMGA1 binding to the cyclin D1 promoter was disclosed for the first time, at least to the best of our knowledge. Taken together, our findings indicate that the IL‑17/HMGA1/cyclin D1 axis plays an important role in NSCLC cell proliferation and may provide new insight into NSCLC pathogenesis and may thus aid in the development of novel therapeutic targets for NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao C, Li Y, Zhang W, Zhao D, Ma L, Ma P, Yang F, Wang Y, Shu Y, Qiu W, Qiu W, et al: IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression. Int J Oncol 52: 1579-1592, 2018.
APA
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P. ... Qiu, W. (2018). IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression. International Journal of Oncology, 52, 1579-1592. https://doi.org/10.3892/ijo.2018.4307
MLA
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P., Yang, F., Wang, Y., Shu, Y., Qiu, W."IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression". International Journal of Oncology 52.5 (2018): 1579-1592.
Chicago
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P., Yang, F., Wang, Y., Shu, Y., Qiu, W."IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression". International Journal of Oncology 52, no. 5 (2018): 1579-1592. https://doi.org/10.3892/ijo.2018.4307
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao C, Li Y, Zhang W, Zhao D, Ma L, Ma P, Yang F, Wang Y, Shu Y, Qiu W, Qiu W, et al: IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression. Int J Oncol 52: 1579-1592, 2018.
APA
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P. ... Qiu, W. (2018). IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression. International Journal of Oncology, 52, 1579-1592. https://doi.org/10.3892/ijo.2018.4307
MLA
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P., Yang, F., Wang, Y., Shu, Y., Qiu, W."IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression". International Journal of Oncology 52.5 (2018): 1579-1592.
Chicago
Zhao, C., Li, Y., Zhang, W., Zhao, D., Ma, L., Ma, P., Yang, F., Wang, Y., Shu, Y., Qiu, W."IL‑17 induces NSCLC A549 cell proliferation via the upregulation of HMGA1, resulting in an increased cyclin D1 expression". International Journal of Oncology 52, no. 5 (2018): 1579-1592. https://doi.org/10.3892/ijo.2018.4307
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