Equol induced apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 but not MCF-7 cells

  • Authors:
    • Eun Jeong Choi
    • Taehee Kim
  • View Affiliations

  • Published online on: March 1, 2008     https://doi.org/10.3892/mmr.1.2.239
  • Pages: 239-244
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To investigate the effects of equol on cell cycle distribution and apoptosis in human breast cancer cells, we first determined the antiproliferative effects of various concentrations of equol (1 nM to 100 µM) on MCF-7 and MDA-MB-453 cells at 24, 48 or 72 h of exposure. Equol significantly inhibited proliferation in MDA-MB-453 cells in a dose- and time-dependent manner. In contrast, equol at low concentrations (<1 µM) stimulated proliferation in MCF-7 cells, with increased expression of proliferating cell nuclear antigen (PCNA), and only inhibited proliferation at a high concentration (100 µM). Similarly, equol treatment of MDA-MB-453 cells resulted in significant cell cycle arrest at the G1/S transition and in the G2/M phase, whereas it caused the slight cell cycle arrest of MCF-7 cells in the G2/M phase after 72 h of treatment. Also, when cells were treated with 50 and 100 µM equol for 72 h, the equol affected cell cycle regulatory proteins more significantly in MDA-MB-453 than in MCF-7 cells. During equol-induced apoptosis, equol increased the number of cells in the sub-G0 phase and enhanced the level of p53. The expression of Bax and cytochrome c, downstream targets of p53, was markedly increased by treatment with higher concentrations of equol. Equol-induced cell cycle arrest and apoptosis apparently involves a p53-dependent pathway in different types of breast cancer cells.

Related Articles

Journal Cover

March-April 2008
Volume 1 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Choi EJ and Kim T: Equol induced apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 but not MCF-7 cells. Mol Med Rep 1: 239-244, 2008.
APA
Choi, E.J., & Kim, T. (2008). Equol induced apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 but not MCF-7 cells. Molecular Medicine Reports, 1, 239-244. https://doi.org/10.3892/mmr.1.2.239
MLA
Choi, E. J., Kim, T."Equol induced apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 but not MCF-7 cells". Molecular Medicine Reports 1.2 (2008): 239-244.
Chicago
Choi, E. J., Kim, T."Equol induced apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 but not MCF-7 cells". Molecular Medicine Reports 1, no. 2 (2008): 239-244. https://doi.org/10.3892/mmr.1.2.239