Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November 2013 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November 2013 Volume 8 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model

  • Authors:
    • Wen-Jiao Huang
    • Fa-Fa Tian
    • Jin-Mei Chen
    • Ting-Hui Guo
    • Yun‑Feng Ma
    • Jia Fang
    • Jing Dang
    • Ming-Yu Song
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Neurology, Putuo Hospital of Shanghai, Shanghai 200062, P.R. China, Department of Neurology, Zhangzhou Hospital, Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China, Department of Neurology, Sanbofuxing Brain Hospital, Beijing 100038, P.R. China, Department of Neurology, 1st People's Hospital of Zhengzhou, Zhengzhou, Henan 450004, P.R. China
  • Pages: 1337-1342
    |
    Published online on: August 28, 2013
       https://doi.org/10.3892/mmr.2013.1660
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Mossy fiber sprouting (MFS) is a pathological phenomenon that is commonly observed in temporal lobe epilepsy (TLE). However, the molecular mechanisms underlying MFS remain unclear. It has been demonstrated that the tau protein is important in the progression of MFS by the regulation of microtubule dynamics and axonal transport, with all of these functions of tau modulated by its site‑specific phosphorylation. Glycogen synthase kinase‑3β (GSK‑3β) is an active kinase that regulates the phosphorylation of tau protein. Therefore, it was hypothesized that GSK‑3β contributes to MFS by phosphorylating tau protein. The aim of the present study was to determine the expression and activity of GSK‑3β at different regions in the rat hippocampus during the pentylenetetrazole (PTZ)-kindling process in order to demonstrate the possible correlation with MFS, and to investigate the involvement of GSK‑3β in epileptogenesis. Male Sprague‑Dawley rats (n=180) were randomly divided into the control and PTZ‑treated groups. The chronic epileptic model was established by intraperitoneal injection of PTZ and the hippocampus was observed for the presence of MFS using Timm staining. GSK‑3β mRNA, protein and activity were analyzed in various regions of the hippocampus using in situ hybridization, immunohistochemistry and immunoprecipitation followed by a kinase assay and liquid scintillation counting, respectively. MFS was observed prior to kindling and an increased distribution of Timm granules were observed in the CA3 region of the PTZ‑treated rats; however, this was not demonstrated in the supragranular layer of the dentate gyrus. The expression of GSK‑3β mRNA and protein, as well as the GSK‑3β activity, increased significantly from 3 days to 4 weeks in the PTZ group, and this was correlated with the progression of MFS in the CA3 area. In addition, it was demonstrated that MFS did not result from TLE. GSK‑3β may therefore be involved in the progression of MFS and is important in epileptogenesis. An understanding of the molecular mechanisms underlying MFS may lead to the identification of a novel therapeutic target to limit epileptogenesis.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Kuo LW, Lee CY, Chen JH, Wedeen VJ, Chen CC, Liou HH and Tseng WY: Mossy fiber sprouting in pilocarpine-induced status epilepticus rat hippocampus: a correlative study of diffusion spectrum imaging and histology. NeuroImage. 41:789–800. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Andrade-Valença LP, Valença MM, Velasco TR, Carlotti CG Jr, Assirati JA, Galvis-Alonso OY, Neder L, Cendes F and Leite JP: Mesial temporal lobe epilepsy clinical and neuropathologic findings of familial and sporadic forms. Epilepsia. 6:1046–1054. 2008.PubMed/NCBI

3 

Sutula TP and Dudek FE: Unmasking recurrent excitation generated by mossy fiber sprouting in the epileptic dentate gyrus: an emergent property of a complex system. Prog Brain Res. 163:541–563. 2007. View Article : Google Scholar

4 

Longo BM and Mello LE: Effect of long-term spontaneous recurrent seizures or reinduction of status epilepticus on the development of supragranular mossy fiber sprouting. Epilepsy Res. 36:233–241. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Johnson GV and Stoothoff WH: Tau phosphorylation in neuronal cell function and dysfunction. J Cell Sci. 117:5721–5729. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Merrick SE, Demoise DC and Lee VM: Site-specific dephosphorylation of tau protein at Ser202/Thr205 in response to microtubule depolymerization in cultured human neurons involves protein phosphatase 2A. J Biol Chem. 271:5589–5594. 1996. View Article : Google Scholar

7 

Pollard H, Khrestchatisky M, Moreau J, Ben-Ari Y and Represa A: Correlation between reactive sprouting and microtubule protein expression in epileptic hippocampus. Neuroscience. 61:773–787. 1994. View Article : Google Scholar : PubMed/NCBI

8 

Palmio J, Suhonen J, Keränen T, Hulkkonen J, Peltola J and Pirttilä T: Cerebrospinal fluid tau as a marker of neuronal damage after epileptic seizure. Seizure. 18:474–477. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Plattner F, Angelo M and Giese KP: The roles of cyclin-dependent kinase 5 and glycogen synthase kinase 3 in tau hyperphosphorylation. J Biol Chem. 281:25457–25465. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Yeste-Velasco M, Folch J, Trullàs R, Abad MA, Enguita M, Pallàs M and Camins A: Glycogen synthase kinase-3 is involved in the regulation of the cell cycle in cerebellar granule cells. Neuropharmacology. 53:295–307. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Zhou FQ and Snider WD: Cell biology. GSK-3β and microtubule assembly in axons. Science. 308:211–214. 2005.

12 

National Institues of Health. Guide for the care and use of laboratory animals. The National Academies Press; Washington, D.C: 1996

13 

Racine RJ: Modification of seizure activity by electrical stimulation. II Motor seizure Electroencephalogr. Clin Neurophysiol. 32:281–294. 1974. View Article : Google Scholar : PubMed/NCBI

14 

Sogawa Y, Monokoshi M, Silveira DC, Cha BH, Cilio MR, McCabe BK, Liu X, Hu Y and Holmes GL: Timing of cognitive deficits following neonatal seizures: relationship to histological changes in the hippocampus. Brain Res Dev Brain Res. 131:73–83. 2001. View Article : Google Scholar

15 

Pierce JP, Melton J, Punsoni M, McCloskey DP and Scharfman HE: Mossy fibers are the primary source of afferent input to ectopic granule cells that are born after pilocarpine-induced seizures. Exp Neurol. 196:316–331. 2005.PubMed/NCBI

16 

Holmes GL, Sarkisian M, Ben-Ari Y and Chevassus-Au-Louis N: Mossy fiber sprouting after recurrent seizures during early development in rats. J Comp Neurol. 404:537–553. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Cross DJ and Cavazos JE: Synaptic reorganization in subiculum and CA3 after early-life status epilepticus in the kainic acid rat model. Epilepsy Res. 73:156–165. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Tian FF, Zeng C, Guo TH, Chen Y, Chen JM, Ma YF, Fang J, Cai XF, Li FR, Wang XH, et al: Mossy fiber sprouting, hippocampal damage and spontaneous recurrent seizures in pentylenetetrazole kindling rat model. Acta Neurol Belg. 109:298–304. 2009.PubMed/NCBI

19 

Jiang H, Guo W, Liang X and Rao Y: Both the establishment and the maintenance of neuronal polarity require active mechanisms: critical roles of GSK-3β and its upstream regulators. Cell. 120:123–135. 2005.PubMed/NCBI

20 

Sen A, Thom M, Martinian L, Harding B, Cross JH, Nikolic M and Sisodiya SM: Pathological tau tangles localize to focal cortical dysplasia in older patients. Epilepsia. 48:1447–1454. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Tian FF, Zeng C, Ma YF, Guo TH, Chen JM, Chen Y, Cai XF, Li FR, Wang XH, Huang WJ and Wang YZ: Potential roles of Cdk5/p35 and tau protein in hippocampal mossy fiber sprouting in the PTZ kindling model. Clin Lab. 56:127–136. 2010.PubMed/NCBI

22 

Linseman DA, Butts BD, Precht TA, Phelps RA, Le SS, Laessig TA, Bouchard RJ, Florez-McClure ML and Heidenreich KA: Glycogen synthase kinase-3beta phosphorylates Bax and promotes its mitochondrial localization during neuronal apoptosis. J Neurosci. 24:9993–10002. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Chen L, Wei Y, Wang X and He R: D-Ribosylated Tau forms globular aggregates with high cytotoxicity. Cell Mol Life Sci. 66:2559–2571. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Huang W, Tian F, Chen J, Guo T, Ma YF, Fang J, Dang J and Song M: GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model. Mol Med Rep 8: 1337-1342, 2013.
APA
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J. ... Song, M. (2013). GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model. Molecular Medicine Reports, 8, 1337-1342. https://doi.org/10.3892/mmr.2013.1660
MLA
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J., Dang, J., Song, M."GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model". Molecular Medicine Reports 8.5 (2013): 1337-1342.
Chicago
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J., Dang, J., Song, M."GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model". Molecular Medicine Reports 8, no. 5 (2013): 1337-1342. https://doi.org/10.3892/mmr.2013.1660
Copy and paste a formatted citation
x
Spandidos Publications style
Huang W, Tian F, Chen J, Guo T, Ma YF, Fang J, Dang J and Song M: GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model. Mol Med Rep 8: 1337-1342, 2013.
APA
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J. ... Song, M. (2013). GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model. Molecular Medicine Reports, 8, 1337-1342. https://doi.org/10.3892/mmr.2013.1660
MLA
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J., Dang, J., Song, M."GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model". Molecular Medicine Reports 8.5 (2013): 1337-1342.
Chicago
Huang, W., Tian, F., Chen, J., Guo, T., Ma, Y., Fang, J., Dang, J., Song, M."GSK‑3β may be involved in hippocampal mossy fiber sprouting in the pentylenetetrazole‑kindling model". Molecular Medicine Reports 8, no. 5 (2013): 1337-1342. https://doi.org/10.3892/mmr.2013.1660
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team