κ-carrageenan induces the disruption of intestinal epithelial Caco-2 monolayers by promoting the interaction between intestinal epithelial cells and immune cells

Jiang,Hai-Yue; Wang,Feng; Chen,Hai‑Min; Yan,Xiao‑Jun

doi:10.3892/mmr.2013.1726

κ-carrageenan induces the disruption of intestinal epithelial Caco-2 monolayers by promoting the interaction between intestinal epithelial cells and immune cells

Authors:
- Hai-Yue Jiang
- Feng Wang
- Hai‑Min Chen
- Xiao‑Jun Yan
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Affiliations: Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Clinical Laboratory, Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, P.R. China
Published online on: October 14, 2013 https://doi.org/10.3892/mmr.2013.1726
Pages: 1635-1642

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Abstract

κ‑carrageenan (κ‑CGN) is an important food additive that has been demonstrated to induce colitis in animal models. In the present study, the effects of κ‑CGN were assessed using an in vitro co‑culture system that contained intestinal epithelial Caco‑2 cells and activated macrophage‑like THP‑1 cells. The results demonstrated that in single cultures of Caco‑2 and THP‑1 cells treated with κ‑CGN, the cytotoxicity and the secretion levels of IL‑1β, IL‑6 and TNF‑α were low. In the co‑culture system, however, κ‑CGN treatment resulted in apoptosis and reduced the transepithelial electrical resistance of the Caco‑2 cell monolayers. The secretion levels of TNF‑α, IL‑1β and IL‑6 from the two cell types were increased significantly by κ‑CGN treatment. Furthermore, pretreatment of the co‑culture system with anti‑TNF receptor 1 antibody suppressed the κ‑CGN‑induced apoptosis and attenuated the changes in the levels of IL‑6 and IL‑1β in the Caco‑2 monolayers. This study indicated that κ‑CGN‑induced TNF‑α secretion is the main contributor to cellular damage in Caco‑2 monolayers exposed to κ‑CGN.

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