Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis

  • Authors:
    • Dongmei Zuo
    • Qing Tang
    • Heng Fan
    • Zhexing Shou
    • Xingxing Liu
    • Dan Cao
    • Zhou Zou
  • View Affiliations

  • Published online on: December 3, 2014     https://doi.org/10.3892/mmr.2014.3038
  • Pages: 2741-2748
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Mesenchymal stem cells (MSCs) inhibit the immune response in vitro and prevent the induction of disease in certain experimental models. As a result, MSC‑mediated therapy is a rapidly growing field of research. However, the efficacy of MSCs in the treatment of inflammatory bowel disease (IBD) has remained to be determined. In the present study, rats with 2,4,6‑trinitrobenzene sulfonic acid (TNBS)‑induced colitis were injected with prepared MSCs (1x106) into the tail vein. Two weeks following intravenous MSC administration, the concentration of tumor necrosis factor‑α (TNF‑α) in the serum was measured by an ELISA. The protein expression of nuclear factor‑κB (NF‑κBp65) in the colonic mucosa was assessed by western blot analysis. mRNA expression of TNF‑α and NF‑κBp65 was determined by reverse‑transcription quantitative polymerase chain reaction. MSCs were shown to exert an immunomodulatory effect on TNBS‑induced colitis and may be of use in the treatment of IBD. In addition, modulation of the NF‑κB‑mediated pro‑inflammatory response may contribute to the underlying mechanism by which MSCs ameliorate the clinical and histological changes associated with IBD.
View Figures
View References

Related Articles

Journal Cover

April-2015
Volume 11 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zuo D, Tang Q, Fan H, Shou Z, Liu X, Cao D and Zou Z: Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Mol Med Rep 11: 2741-2748, 2015.
APA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., & Zou, Z. (2015). Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Molecular Medicine Reports, 11, 2741-2748. https://doi.org/10.3892/mmr.2014.3038
MLA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11.4 (2015): 2741-2748.
Chicago
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11, no. 4 (2015): 2741-2748. https://doi.org/10.3892/mmr.2014.3038