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Article

Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis

  • Authors:
    • Dongmei Zuo
    • Qing Tang
    • Heng Fan
    • Zhexing Shou
    • Xingxing Liu
    • Dan Cao
    • Zhou Zou
  • View Affiliations / Copyright

    Affiliations: Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 2741-2748
    |
    Published online on: December 3, 2014
       https://doi.org/10.3892/mmr.2014.3038
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Abstract

Mesenchymal stem cells (MSCs) inhibit the immune response in vitro and prevent the induction of disease in certain experimental models. As a result, MSC‑mediated therapy is a rapidly growing field of research. However, the efficacy of MSCs in the treatment of inflammatory bowel disease (IBD) has remained to be determined. In the present study, rats with 2,4,6‑trinitrobenzene sulfonic acid (TNBS)‑induced colitis were injected with prepared MSCs (1x106) into the tail vein. Two weeks following intravenous MSC administration, the concentration of tumor necrosis factor‑α (TNF‑α) in the serum was measured by an ELISA. The protein expression of nuclear factor‑κB (NF‑κBp65) in the colonic mucosa was assessed by western blot analysis. mRNA expression of TNF‑α and NF‑κBp65 was determined by reverse‑transcription quantitative polymerase chain reaction. MSCs were shown to exert an immunomodulatory effect on TNBS‑induced colitis and may be of use in the treatment of IBD. In addition, modulation of the NF‑κB‑mediated pro‑inflammatory response may contribute to the underlying mechanism by which MSCs ameliorate the clinical and histological changes associated with IBD.
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1 

Schirbel A and Fiocchi C: Inflammatory bowel disease: Established and evolving considerations on its etiopathogenesis and therapy. J Dig Dis. 11:266–276. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Baumgart DC and Carding SR: Inflammatory bowel disease: cause and immunobiology. Lancet. 369:1627–1640. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Hisamatsu T, Kanai T, Mikami Y, et al: Immune aspects of the pathogenesis of inflammatory bowel disease. Pharmacol Ther. 137:283–297. 2013. View Article : Google Scholar

4 

Műzes G, Molnár B, Tulassay Z and Sipos F: Changes of the cytokine profile in inflammatory bowel diseases. World J Gastroenterol. 18:5848–5861. 2012. View Article : Google Scholar :

5 

Xavier RJ and Podolsky DK: Unravelling the pathogenesis of inflammatory bowel disease. Nature. 448:427–434. 2007. View Article : Google Scholar : PubMed/NCBI

6 

de Zoeten E and Mamula P: What are the guidelines for using biologics in pediatric patients? Inflamm Bowel Dis. 14(Suppl 2): S259–S261. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Taba Taba Vakili S, Taher M and Ebrahimi Daryani N: Update on the management of ulcerative colitis. Acta Med Iran. 50:363–372. 2012.PubMed/NCBI

8 

Parekkadan B and Milwid JM: Mesenchymal stem cells as therapeutics. Annu Rev Biomed Eng. 12:87–117. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Meyerrose T, Olson S, Pontow S, et al: Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors. Adv Drug Deliv Rev. 62:1167–1174. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Le Blanc K, Tammik C, Rosendahl K, Zetterberg E and Ringdén O: HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells. Exp Hematol. 31:890–896. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Le Blanc K, Tammik L, Sundberg B, Haynesworth SE and Ringdén O: Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol. 57:11–20. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Dalal J, Gandy K and Domen J: Role of mesenchymal stem cell therapy in Crohn’s disease. Pediatr Res. 71:445–451. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Ringdén O, Uzunel M, Rasmusson I, et al: Mesenchymal stem cells for treatment of therapy-resistant graft-versus-host disease. Transplantation. 81:1390–1397. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Sun S, Guo Z, Xiao X, et al: Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells. 21:527–535. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Morris GP, Beck PL, Herridge MS, et al: Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 96:795–803. 1989.PubMed/NCBI

16 

Davaatseren M, Hwang JT, Park JH, et al: Poly-γ-glutamic acid attenuates angiogenesis and inflammation in experimental colitis. Mediators Inflamm. 2013:9823832013. View Article : Google Scholar

17 

González R, Rodríguez S, Romay C, et al: Anti-inflammatory activity of phycocyanin extract in acetic acid-induced colitis in rats. Parmacol Res. 39:55–59. 1999.

18 

Guo Y, Su L, Wu J, et al: Assessment of the green fluorescence protein labeling methods for tracking implanted mesenchymal stem cells. Cytotechnology. 64:391–401. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Yi T and Song SU: Immunomodulatory properties of mesenchymal stem cells and their therapeutic applications. Arch Pharm Res. 35:213–221. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Uccelli A, Moretta L and Pistoia V: Mesenchymal stem cells in health and disease. Nat Rev Immunol. 8:726–736. 2008. View Article : Google Scholar

21 

Tolar J, Le Blanc K, Keating A and Blazar BR: Concise review: hitting the right spot with mesenchymal stromal cells. Stem Cells. 28:1446–1455. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Krampera M, Cosmi L, Angeli R, et al: Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells. 24:386–398. 2006. View Article : Google Scholar

23 

Tanaka F, Tominaga K, Ochi M, et al: Exogenous administration of mesenchymal stem cells ameliorates dextran sulfate sodium-induced colitis via anti-inflammatory action in damaged tissue in rats. Life Sci. 83:771–779. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Cooper HS, Murthy SN, Shah RS and Sedergran DJ: Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 69:238–249. 1993.PubMed/NCBI

25 

Idriss HT and Naismith JH: TNF alpha and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech. 50:184–195. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Locksley RM, Killeen N and Lenardo MJ: The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell. 104:487–501. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Smith CA, Farrah T and Goodwin RG: The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death. Cell. 76:959–962. 1994. View Article : Google Scholar : PubMed/NCBI

28 

Ogata H and Hibi T: Cytokine and anti-cytokine therapies for inflammatory bowel disease. Curr Pharm Des. 9:1107–1113. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Zheng L, Fisher G, Miller RE, et al: Induction of apoptosis in mature T cells by tumour necrosis factor. Nature. 377:348–351. 1995. View Article : Google Scholar : PubMed/NCBI

30 

Noti M, Corazza N, Mueller C, Berger B and Brunner T: TNF suppresses acute intestinal inflammation by inducing local glucocorticoid synthesis. J Exp Med. 207:1057–1066. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Olson TS, Reuter BK, Scott KG, et al: The primary defect in experimental ileitis originates from a nonhematopoietic source. J Exp Med. 203:541–552. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Reimund JM, Wittersheim C, Dumont S, et al: Mucosal inflammatory cytokine production by intestinal biopsies in patients with ulcerative colitis and Crohn’s disease. J Clin Immunol. 16:144–150. 1996. View Article : Google Scholar : PubMed/NCBI

33 

Armuzzi A, Lionetti P, Blandizzi C, Caporali R, et al: Anti-TNF agents as therapeutic choice in immune-mediated inflammatory diseases: focus on adalimumab. Int J Immunopathol Pharmacol. 27(1 Suppl): 11–32. 2014.PubMed/NCBI

34 

Chen FE and Ghosh G: Regulation of DNA binding by Rel/NF-kappaB transcription factors: structural views. Oncogene. 18:6845–6852. 1999. View Article : Google Scholar : PubMed/NCBI

35 

Barnes PJ and Karin M: Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 336:1066–1071. 1997. View Article : Google Scholar : PubMed/NCBI

36 

DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E and Karin M: A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 388:548–554. 1997. View Article : Google Scholar : PubMed/NCBI

37 

Schmitz ML, Bacher S and Kracht M: I kappa B-independent control of NF-kappa B activity by modulatory phosphorylations. Trends Biochem Sci. 26:186–190. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Pizarro TT and Cominelli F: Cytokine therapy for Crohn’s disease: advances in translational research. Annu Rev Med. 58:433–444. 2007. View Article : Google Scholar

39 

Elson CO, Cong Y and Weaver CT: Monoclonal anti-interleukin 23 reverses active colitis in a T cell-mediated model in mice. Gastroenterol. 132:2359–2370. 2007. View Article : Google Scholar

40 

Atreya R, Mudter J, Finotto S, et al: Blockade of interleukin 6 trans signaling suppresses T-cell resistance against apoptosis in chronic intestinal inflammation: evidence in crohn disease and experimental colitis in vivo. Nat Med. 6:583–588. 2000. View Article : Google Scholar : PubMed/NCBI

41 

Suenaert P, Bulteel V, Lemmens L, et al: Anti-tumor necrosis factor treatment restores the gut barrier in Crohn’s disease. Am J Gastroenterol. 97:2000–2004. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zuo D, Tang Q, Fan H, Shou Z, Liu X, Cao D and Zou Z: Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Mol Med Rep 11: 2741-2748, 2015.
APA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., & Zou, Z. (2015). Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Molecular Medicine Reports, 11, 2741-2748. https://doi.org/10.3892/mmr.2014.3038
MLA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11.4 (2015): 2741-2748.
Chicago
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11, no. 4 (2015): 2741-2748. https://doi.org/10.3892/mmr.2014.3038
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo D, Tang Q, Fan H, Shou Z, Liu X, Cao D and Zou Z: Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Mol Med Rep 11: 2741-2748, 2015.
APA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., & Zou, Z. (2015). Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis. Molecular Medicine Reports, 11, 2741-2748. https://doi.org/10.3892/mmr.2014.3038
MLA
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11.4 (2015): 2741-2748.
Chicago
Zuo, D., Tang, Q., Fan, H., Shou, Z., Liu, X., Cao, D., Zou, Z."Modulation of nuclear factor-κB-mediated pro-inflammatory response is associated with exogenous administration of bone marrow-derived mesenchymal stem cells for treatment of experimental colitis". Molecular Medicine Reports 11, no. 4 (2015): 2741-2748. https://doi.org/10.3892/mmr.2014.3038
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