Curcumin ameliorates high‑fat diet‑induced spermatogenesis dysfunction

Introduction

Infertility is defined as when couples who have an active sex life without using protective measures for >1 year, fail to get pregnant, which affects 10–15% (1,2). Of this, ~25–30% are infertile couples due to problems with the man (3). Obesity, an acknowledged major risk factor for male infertility (4–6), leads to decreased sperm number and reproductive dysfunction (7,8). Germ cell apoptosis in the testis is a key pathophysiological process in obesity-induced male spermatogenesis dysfunction (9), therefore inhibiting apoptosis in the testis may improve male spermatogeneses function.

Curcumin, a type of natural active ingredient derived from rhizoma of Curcuma, has protective effects in a series of diseases, including cardiovascular disease (10,11), cancer, Alzheimer's disease (12) and diabetes (13). Curcumin has been previously found to serve a significant role in antioxidant, antimutative, anti-inflammatory and antitumorigenic responses (14–17), and recent studies indicated that curcumin can ameliorate high-glucose-induced neural defects by suppressing cellular stress and apoptosis (18). The present study investigated the hypothesis that curcumin treatment can improve spermatogenesis dysfunction induced by a high-fat diet (HFD). Testis/body weight, histological analysis and serum hormone levels were determined to reflect spermatogenesis function of adult rats. Since germ cell apoptosis is an important process during HFD-induced spermatogenesis dysfunction (9), apoptosis associated proteins, Fas, B-cell lymphoma (Bcl)-xl, Bcl-associated X protein (Bax) and cleaved-caspase 3 were also assessed.

Materials and methods

Animal care and treatment

A total of 30 male Sprague-Dawley rats (permit number, 42000500002649), weighing 200–250 g were used in the present study. All animals experiments performed in the present study were approved by the Institutional Animal Care and Use Committee of Renmin Hospital of Wuhan University (Wuhan, China). The animals were allowed free access to food and water at all times and were maintained on a 12 h light/dark cycle at a controlled temperature (20–25°C) and humidity (50±5%), which was also pathogen-free. The composition of the HFD is shown in Table I. Curcumin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved by olive oil and administered orally by oral gavage. The rats were randomly divided into three groups: i) Control; ii) HFD; iii) curcumin treatment groups. All rats were subjected to a HFD for 8 weeks, with the exception of those in the control group ad libitum feeding. The rats in curcumin treatment group were orally administered curcumin treatment (100 mg/kg/day) for 8 weeks, while in the control and HFD groups, an identical volume vehicle was used. Following treatment, the rats were anesthetized with sodium pentobarbital (45 mg/kg) intraperitoneally and blood samples were collected from the abdominal aorta. The rats were subsequently euthanized with 200 mg/kg sodium pentobarbital intraperitoneally and the testes were collected to calculate testis/body weight.

Table I Macronutrient composition of diets for rats.

Table I

Macronutrient composition of diets for rats.

Nutrient	Control		High-fat diet
	g, %	kJ, %	g, %	kJ, %
Protein	20	19	20	14
Carbohydrate	76	72	45	31
Saturated fat	4	9	35	55
kJ/g	17.5		24.1

Histological analysis

The collected testis tissues were fixed with 4% paraformaldehyde, dehydrated and embedded in paraffin (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The testis tissue sections (5 μm) were sectioned with a microtome and stained with hematoxylin and eosin to examine the morphology. The tissue sections (5 μm) were observed under light microscopy (Nikon E100; Nikon, Tokyo, Japan), and the photomicrographs were obtained by Photo Imaging System (Canon 600D; Canon, Tokyo, Japan). The diameter of seminiferous tubules was measured by Image-Pro Plus 6.0 (Media Cybernetics, Inc., Rockville, MD, USA). In each group, 120 seminiferous tubules (5 fields/rat, randomized 4 seminiferous tubules/field) in 6 rats' testes were counted. Additionally, 30 fields (5 fields/rat) in 6 rats/group were randomly selected to count spermatogenetic cells and interstitial cells.

Hormone levels assay

Blood samples were obtained from the abdominal aorta. Following centrifugation (1,506 × g) for 10 min at 4°C, the sera were obtained for further detection. Serum levels of estradiol (E2), testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH) and leptin were measured using kits, according to the manufacturer's protocol (Elabscience Biotechnology Co., Ltd., Wuhan, China).

Immunohistochemistry

The protein expression levels of Fas, Bcl-xl and leptin receptors were tested by immunohistochemistry. The sections were deparaffinized and were subsequently boiled for 15 min in sodium citrate buffer for antigen retrieval. Following elimination of internal peroxidase activity, the sections were incubated with rabbit anti-Fas (1:50; cat no. BA0408), rabbit anti-leptin-receptor (1:50; cat no. BA1233; Wuhan Boster Biological Technology, Ltd., Wuhan, China) and rabbit anti-Bcl-xl (1:100; cat. no. 10783-AP; Proteintech Group, Inc., Chicago, IL, USA) antibodies at 4°C overnight. The tissue sections were exposed to biotinylated sheep anti-rabbit immunoglobulin G solution (Proteintech Group, Inc.) at 37°C for 30 min and were subsequently incubated with horseradish peroxidase-labeled streptavidin (Proteintech Group, Inc.) at 37°C for 30 min. Finally, the tissue sections were observed under light microscopy (Nikon E100; Nikon), and the photomicrographs were obtained by Photo Imaging System (Canon 600D; Canon). In each group 30 fields (5 fields/rat) in 6 rat testis were randomly selected. Positive expression was assessed using Image-Pro Plus 6.0 and a mean of the integrated optical density was obtained.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)

Tissue sections were processed, according to the manufacturer's protocol for the TUNEL kit (Roche Applied Science, Indianapolis, IN, USA). Positively labeled nuclei were stained a brown color, while negatively labeled nuclei were blue. A total of 30 fields were randomly selected in each group (5 fields/rat) and 100 cells were counted in each field under a microscope (Nikon E100; Nikon), and the number of positive cells was recorded. The apoptosis index (number of apoptotic cells in each field/100) was computed for each field.

Western blot analysis

Lysis buffer (720 μl radioimmunoprecipitation buffer, 100 mmol/l PMSF, 100 μl cocktail, 100 μl Phos-stop, 20 mmol/l NaF and 100 mmol/l Na3VO4 in 1 ml) was used to extract the total proteins from fresh testicular tissues. The protein concentrations were tested using the Bicinchoninic Acid Protein Assay kit (Thermo Fisher Scientific, Inc.) and a plate reader (Bio-Tek Instruments, Inc., Winooski, VT, USA). Equal quantities of protein (30 μg) were separated with denaturing sodium dodecyl sulphate 10% polyacrylamide gels under reducing and denaturing conditions and were subsequently transferred onto a polyvinylidene difluoride (PVDF) membrane (EMD Millipore, Billerica, MA, USA). The PVDF membrane was blocked with 5% (w/v) non-fat milk and 0.1% Tween in Tris-buffered saline (pH 7.4) at room temperature for 1 h. Following blocking, the membrane was incubated overnight at 4°C with the following rabbit primary antibodies: Anti-GAPDH antibody (1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; cat. no. sc-25778), anti-Bax antibody (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA; cat. no. 14796) and anti-cleaved-caspase 3 antibody (1:1,000; Cell Signaling Technology, Inc.; cat. no. 9664). Following the incubation with primary antibody, the membrane was incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, Lincoln, NE, USA; cat. no. 926-32211) for 1 h. Finally, the membrane was scanned using a two-color infrared imaging system (Odyssey, LI-COR Biosciences). Densitometric analysis was performed by Odyssey, as previous described (19), and the results were expressed as the ratio between targeted proteins and GAPDH band intensities.

Statistical analysis

All statistical analyses were performed with SPSS 19.0 (IBS SPSS, Chicago, IL, USA). All data are expressed as the mean ± standard error of the mean. Multiple group comparison was performed by analysis of variance, followed by the post-hoc least significant difference test assuming equal variances; otherwise Tamhane's T2 post-hoc test. All statistical analyses were two-sided. P<0.05 was considered to indicate a statistically significant difference.

Results

Histomorphological changes

Following a HFD for 8 weeks, the testis/body weight was reduced and treatment with curcumin increased the testis/body weight (Table II; Fig. 1). The testicular structure of the control group under the light microscope manifested as larger seminiferous tubules, abundant spermatogenetic and interstitial cells. As shown in Fig. 1, atrophic seminiferous tubules and more vacuoles were observed in the HFD group. Notably, decreased spermatogenetic and interstitial cells were observed in the HFD group. However, these histological changes were improved following treatment with curcumin (Table III; Fig. 1).

Figure 1 Oral treatment with curcumin prevents HFD-induced male spermatogenesis dysfunction. (A) Hematoxylin and eosin staining of the testis in the three groups (n=6; magnification, ×100 and ×400; scale bar, 100 and 20 μm on the left and right, respectively). (B) Testicular/body weight was determined for the three groups (n=10). (C–E) Quantitative analysis of morphological changes of the testis in three groups are presented (n=6). The data are expressed as the mean ± standard error of the mean (*P<0.05, HFD vs. control group; #P<0.05, curcumin treatment vs. HFD group. HFD, high-fat diet.

Table II Testicular weight and testicular/body weight of the three groups.

Table II

Testicular weight and testicular/body weight of the three groups.

Group	n	Body weight (g)	Testicular weight (g)	Testicular/body weight (g/kg)
Control	10	315.80±14.74	3.14±0.21	9.97±0.93
High-fat diet	10	371.40±12.05a	2.70±0.16a	7.27±0.35a
Curcumin	10	353.20±5.76	2.90±0.12b	8.21±0.38b

{ label (or @symbol) needed for fn[@id='tfn1-mmr-14-04-3588'] } The data are expressed as the mean ± standard error of the mean.

a P<0.05 vs. control group;

b P<0.05 vs. high-fat diet group.

Table III Comparison of diameters of seminiferous tubules and the quantity of spermatogenetic cells and interstitial cells in the three groups.

Table III

Comparison of diameters of seminiferous tubules and the quantity of spermatogenetic cells and interstitial cells in the three groups.

Group	n	Seminiferous tubule diameter (μm)	Quantity of spermatogenetic cells	Quantity of interstitial cells
Control	6	395.08±19.91	25.40±1.14	8.16±0.29
High-fat diet	6	273.70±16.55a	14.80±1.79a	4.16±0.12a
Curcumin	6	319.94±20.55b	20.60±1.14b	5.37±0.11b

{ label (or @symbol) needed for fn[@id='tfn4-mmr-14-04-3588'] } The data are expressed as the mean ± standard error of the mean.

a P<0.05 vs. control group;

b P<0.05 vs. HFD group.

Determination of endogenous hormone

Rats fed with an HFD exhibited abnormal serum hormone levels, manifested as reduced serum levels of T, FSH and LH, and increased serum levels of leptin and E2. Serum hormone levels were observed to be restored following treatment with curcumin (Figs. 2 and 5).

Figure 2 Curcumin treatment improves defective serum hormone levels of (A) T, (B) E2, (C) FSH and (D) LH induced by HFD. (n=8; *P<0.05, HFD vs. control group; #P<0.05, curcumin treatment group vs. HFD group). FSH, follicle-stimulating hormone; LH, luteinizing hormone; T, testosterone; E2, estradiol; HFD, high-fat diet.

Figure 5 Curcumin treatment decreases the expression of leptin receptors and reduces serum leptin level. (A) Immunohistochemical staining for the expression of leptin receptors in the testis (n=6; magnification ×100 and ×400; scale bar 100 and 20 μm on the upper and lower panels, respectively). Arrows indicate positive expression. (B) The mean OD of leptin receptors (n=6) and (C) serum leptin levels (n=8) were quantified. The data are expressed as the mean ± standard error of the mean (*P<0.05, HFD vs. control; #P<0.05, curcumin vs. HFD group). HFD, high-fat diet; OD, optical density.

Expression levels of apoptosis associated proteins and leptin receptors

A HFD resulted in more apoptotic cells in the testis, characterized by elevated expression levels of Fas, Bax and cleaved-caspase 3, and reduced expression of Bcl-xl. Following treatment with curcumin, these changes were attenuated (Figs. 3 and 4). The expression of leptin receptors was found to be increased in the testis of rats fed a HFD by immunohistochemistry staining, and curcumin treatment reduced these increased leptin receptors (Fig. 5).

Figure 3 Curcumin supplementation attenuates apoptosis in the testis. (A) Immunohistochemical staining for the protein expression levels of Bcl-xl and Fas, and TUNEL for the apoptotic cells in the testis (n=6; magnification ×100 and ×400; scale bar 100 and 20 μm on the upper and lower panels, respectively). Bcl-xl and Fas are predominantly observed in the interstitial cells. Arrows indicate positive expression. (B–D) Quantitative analysis of AI and the OD (n=6). The data are expressed as the mean ± standard error of the mean (*P<0.05, HFD vs. control group; #P<0.05, curcumin vs. HFD group). HFD, high-fat diet; Bcl, B-cell lymphoma; OD, optical density; AI, apoptosis index; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.

Figure 4 Curcumin treatment reduces apoptosis-associated protein expression levels of Bax and cleaved-cas-3. (A) Western blotting analysis of the protein expression levels of Bax and cleaved-cas-3 was assessed in the testis tissues from the three groups (n=4). The fold-change in protein expression levels of (B) Bax and (C) cleaved-cas-3 was quantified relative to the expression of GAPDH. The data are expressed as the mean ± standard error of the mean (*P<0.05, HFD vs. control group; #P<0.05, curcumin vs. HFD group. HFD, high-fat diet; cas, caspase; Bax, B-cell lymphoma associated X protein.

Discussion

In order to investigate the effects of curcumin on male spermatogenesis function, the present study subjected the rats to a HFD to induce spermatogenesis dysfunction. The present results revealed that spermatogenesis function was disturbed in rats fed with a HFD, characterized as decreased testis/body weight, atrophied seminiferous tubules, reduced spermatogenetic and interstitial cells, and abnormal hormone levels, which is consistent with a previous study (7).

Curcumin has been demonstrated to have a series of pharmacological actions, including antioxidant, antimutative, anti-inflammatory and antitumorigenic actions (14–17). Over the last decade, the therapeutic effects of curcumin on various cancer types and Alzheimer's disease have been confirmed by clinical trials (20). The present study found that curcumin treatment improved atrophied testes manifested as increased testis/body weight, increased the diameter of seminiferous tubules, and increased the number of spermatogenetic and interstitial cells. Additionally, curcumin treatment improved the abnormal hormone levels. Taken together, curcumin had protective effects in HFD-induced spermatogenesis dysfunction.

The balance between cell proliferation and cell death is important in spermatogenesis function. As a type of cell death, apoptosis of testicular germ cells was an important physiological mechanism in regulating the germ cell population (21–24). Increased germ cell apoptosis was observed in testicular injury induced by stimuli (25–28), and additionally, suppressing apoptosis improved the sperm count (29–33). As shown in the present results, curcumin treatment decreased the protein expression levels of Fas, Bax and cleaved-caspase 3 and increased the expression of Bcl-xl. Notably, curcumin treatment reduced germ cell apoptosis induced by a HFD.

Leptin, also synthesized by seminiferous tubules (34,35), was found to have important effects on reproductive function (36), which has been confirmed by sterile leptin deficient ob mice (37). Additionally, leptin directly acted on the testis and modulated spermatogenesis (38). In addition, leptin served an inhibitory role in testicular steroidogenesis (39,40) and influenced the weight of the testis, the diameter of seminiferous tubules and the number of germ cells (41). In the present study, the leptin receptors in the testis and serum leptin level were increased in rats subjected to a HFD. Treatment with curcumin downregulated the increased levels of leptin receptors and the serum leptin level.

In conclusion, the present study revealed that curcumin reduced HFD-induced spermatogenesis dysfunction and apoptosis. Curcumin may be a potential novel therapeutic medicine for male patients suffering from obesity-induced infertility.

Acknowledgments

The present study was partially supported by the Key Research Project of the Ministry of Public Security (no. 2010 ZDYJHBST007).

References