Effect of isosecotanapartholide isolated from Artemisia princeps Pampanini on IL‑33 production and STAT‑1 activation in HaCaT keratinocytes

  • Authors:
    • Chang Taek Oh
    • Yu‑Jin Jang
    • Tae‑Rin Kwon
    • Songi Im
    • Soon Re Kim
    • Joon Seok
    • Gun‑Yong Kim
    • Young‑Heui Kim
    • Seog Kyun Mun
    • Beom Joon Kim
  • View Affiliations

  • Published online on: March 9, 2017     https://doi.org/10.3892/mmr.2017.6306
  • Pages: 2681-2688
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate the anti‑inflammatory effect and mechanism of action of isosecotanapartholide (ISTP), isolated from Artemisia princeps Pampanini extract (APE). The effects of ISTP and APE on the proliferation of human keratinocytes following stimulation by tumor necrosis factor‑α/interferon‑γ were assessed. ISTP and APE downregulated the expression levels of signal transducer and activator of transcription‑1 (STAT‑1), and reduced interleukin‑33 (IL‑33) production. ISTP and APE inhibited the mRNA expression levels of thymus and activation‑regulated chemokine (TARC/CCL17) in a dose‑dependent manner. Western blot analysis demonstrated that ISTP and APE dose‑dependently inhibited protein expression levels of intercellular adhesion molecule‑1 and phosphorylation of STAT‑1. The results of the present study indicate that ISTP may inhibit TARC/CCL17 production in human epidermal keratinocytes via the STAT‑1 signaling pathway and may be associated with the inhibition of IL‑33 production. The current study indicated that ISTP is an active component in APE and may be a potential therapeutic agent for the treatment of inflammatory skin disorders.
View Figures
View References

Related Articles

Journal Cover

May-2017
Volume 15 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Oh CT, Jang YJ, Kwon TR, Im S, Kim SR, Seok J, Kim GY, Kim YH, Mun SK, Kim BJ, Kim BJ, et al: Effect of isosecotanapartholide isolated from Artemisia princeps Pampanini on IL‑33 production and STAT‑1 activation in HaCaT keratinocytes. Mol Med Rep 15: 2681-2688, 2017.
APA
Oh, C.T., Jang, Y., Kwon, T., Im, S., Kim, S.R., Seok, J. ... Kim, B.J. (2017). Effect of isosecotanapartholide isolated from Artemisia princeps Pampanini on IL‑33 production and STAT‑1 activation in HaCaT keratinocytes. Molecular Medicine Reports, 15, 2681-2688. https://doi.org/10.3892/mmr.2017.6306
MLA
Oh, C. T., Jang, Y., Kwon, T., Im, S., Kim, S. R., Seok, J., Kim, G., Kim, Y., Mun, S. K., Kim, B. J."Effect of isosecotanapartholide isolated from Artemisia princeps Pampanini on IL‑33 production and STAT‑1 activation in HaCaT keratinocytes". Molecular Medicine Reports 15.5 (2017): 2681-2688.
Chicago
Oh, C. T., Jang, Y., Kwon, T., Im, S., Kim, S. R., Seok, J., Kim, G., Kim, Y., Mun, S. K., Kim, B. J."Effect of isosecotanapartholide isolated from Artemisia princeps Pampanini on IL‑33 production and STAT‑1 activation in HaCaT keratinocytes". Molecular Medicine Reports 15, no. 5 (2017): 2681-2688. https://doi.org/10.3892/mmr.2017.6306