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Article Open Access

Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells

  • Authors:
    • Shang‑Ze Li
    • Jian‑Wei Ren
    • Jing Fei
    • Xiao‑Dong Zhang
    • Run‑Lei Du
  • View Affiliations / Copyright

    Affiliations: Medical Science Research Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Tibet University Medical College, Lhasa, Tibet 850000, P.R. China, Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 901-908
    |
    Published online on: December 3, 2018
       https://doi.org/10.3892/mmr.2018.9717
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Abstract

Cordycepin, or 3'‑deoxyadenosine, is a derivative of the nucleoside adenosine. Initially extracted from the fungus Cordyceps militaris, cordycepin exhibits antitumor activity against certain cancer cell lines; however, the mechanism by which cordycepin counteracts colorectal cancer (CRC) remains poorly understood. The aim of the present study was to explore the underlying mechanisms of cordycepin against human CRC. To investigate the molecular mechanisms of cordycepin against colon cancer and in driving apoptosis, p53 and Bcl‑2‑like protein 4‑null (Bax‑/‑) colon cancer HCT116 cell lines were used. Cell viability and growth were repressed in a dose‑dependent manner in cells treated with cordycepin. Treatment with cordycepin resulted in increased apoptosis in HCT116 cells; however, flow cytometic analysis demonstrated that apoptosis was notably decreased in the Bax‑/‑ HCT116 cell lines, but not in the p53‑/‑ HCT116 cell lines. Furthermore, cordycepin exposure resulted in the translocation of Bax from the cytosol to the mitochondria and the subsequent release of cytochrome c from the mitochondria. Results from the present study demonstrated that cordycepin inhibited colon cancer cell growth in vitro and this appears to be through the endogenous Bax‑dependent mitochondrial apoptosis pathway, which suggested a molecular mechanism for cordycepin against human colon cancer. These results indicated the possibility of cordycepin as a novel drug for the prevention of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li SZ, Ren JW, Fei J, Zhang XD and Du RL: Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells. Mol Med Rep 19: 901-908, 2019.
APA
Li, S., Ren, J., Fei, J., Zhang, X., & Du, R. (2019). Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells. Molecular Medicine Reports, 19, 901-908. https://doi.org/10.3892/mmr.2018.9717
MLA
Li, S., Ren, J., Fei, J., Zhang, X., Du, R."Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells". Molecular Medicine Reports 19.2 (2019): 901-908.
Chicago
Li, S., Ren, J., Fei, J., Zhang, X., Du, R."Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells". Molecular Medicine Reports 19, no. 2 (2019): 901-908. https://doi.org/10.3892/mmr.2018.9717
Copy and paste a formatted citation
x
Spandidos Publications style
Li SZ, Ren JW, Fei J, Zhang XD and Du RL: Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells. Mol Med Rep 19: 901-908, 2019.
APA
Li, S., Ren, J., Fei, J., Zhang, X., & Du, R. (2019). Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells. Molecular Medicine Reports, 19, 901-908. https://doi.org/10.3892/mmr.2018.9717
MLA
Li, S., Ren, J., Fei, J., Zhang, X., Du, R."Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells". Molecular Medicine Reports 19.2 (2019): 901-908.
Chicago
Li, S., Ren, J., Fei, J., Zhang, X., Du, R."Cordycepin induces Bax‑dependent apoptosis in colorectal cancer cells". Molecular Medicine Reports 19, no. 2 (2019): 901-908. https://doi.org/10.3892/mmr.2018.9717
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