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Article

Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway

  • Authors:
    • Yongqing Yang
    • Rongsheng Lu
    • Fangfang Gao
    • Jie Zhang
    • Fenglan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Biological Science, College of Life Science, Shanxi Normal University, Linfen, Shanxi 041000, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2603-2614
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    Published online on: April 10, 2020
       https://doi.org/10.3892/mmr.2020.11070
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Abstract

Lipolysis is closely associated with obesity and insulin resistance. Berberine (BBR), a natural alkaloid derived from Coptis chinensis, has been shown to regulate lipolysis and improve insulin resistance. However, the underlying mechanism remains unclear. The present results suggested that BBR stimulated lipolysis in porcine adipocytes in a dose‑ and time‑dependent manner, which was independent of the cAMP/protein kinase A pathway. Further experimental results indicated that BBR increased phosphorylation levels of AMP‑activated protein kinase (AMPK) and adipose triglyceride lipase (ATGL), along with downregulation of Perilipin A. The AMPK inhibitor compound C significantly reversed the effect of BBR on lipolysis, Perilipin A expression and ATGL phosphorylation. Furthermore, BBR promoted expression levels of genes related to fatty acid oxidation, such as peroxisome proliferator‑activated receptor γ coactivator‑1α, mitochondrial transcription factor A, carnitine palmitoyl‑transferase‑1 and uncoupling protein 2, which were abrogated by AMPKα1 knockdown. Moreover, it was found that BBR‑induced lipolysis did not elevate serine phosphorylation of insulin receptor substrate‑1 to block insulin signaling. Collectively, the present results suggested that BBR induced lipolysis in porcine adipocytes via a pathway that involves AMPK activation, but does not cause insulin resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Lu R, Gao F, Zhang J and Liu F: Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway. Mol Med Rep 21: 2603-2614, 2020.
APA
Yang, Y., Lu, R., Gao, F., Zhang, J., & Liu, F. (2020). Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway. Molecular Medicine Reports, 21, 2603-2614. https://doi.org/10.3892/mmr.2020.11070
MLA
Yang, Y., Lu, R., Gao, F., Zhang, J., Liu, F."Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway". Molecular Medicine Reports 21.6 (2020): 2603-2614.
Chicago
Yang, Y., Lu, R., Gao, F., Zhang, J., Liu, F."Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway". Molecular Medicine Reports 21, no. 6 (2020): 2603-2614. https://doi.org/10.3892/mmr.2020.11070
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Lu R, Gao F, Zhang J and Liu F: Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway. Mol Med Rep 21: 2603-2614, 2020.
APA
Yang, Y., Lu, R., Gao, F., Zhang, J., & Liu, F. (2020). Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway. Molecular Medicine Reports, 21, 2603-2614. https://doi.org/10.3892/mmr.2020.11070
MLA
Yang, Y., Lu, R., Gao, F., Zhang, J., Liu, F."Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway". Molecular Medicine Reports 21.6 (2020): 2603-2614.
Chicago
Yang, Y., Lu, R., Gao, F., Zhang, J., Liu, F."Berberine induces lipolysis in porcine adipocytes by activating the AMP‑activated protein kinase pathway". Molecular Medicine Reports 21, no. 6 (2020): 2603-2614. https://doi.org/10.3892/mmr.2020.11070
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