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Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis

  • Authors:
    • Linmei Zhu
    • Ruixia Chen
    • Chunlin Jiang
    • Qingsheng Xie
    • Wenshuai Zhao
    • Xiaohong Gao
    • Haiming Huang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Guangdong Clifford Hospital, Guangzhou, Guangdong 511495, P.R. China, Department of Anesthesiology, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Gynecology, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 554
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    Published online on: June 2, 2021
       https://doi.org/10.3892/mmr.2021.12193
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Abstract

Long non‑coding RNA ILF3 divergent transcript (ILF3‑AS1) displays a tumor‑suppressing effect. StarBase predicted that the potential target microRNA (miR) of ILF3‑AS1 was miR‑454‑3p; therefore, the present study investigated the effect of ILF3‑AS1 and its target miR‑454‑3p on cervical cancer (CC). Gene Expression Profiling Interactive Analysis was used to predict the expression of ILF3‑AS1 in CC and the overall survival rate of patients. The present study demonstrated that ILF3‑AS1 expression was significantly downregulated in human CC tissues and cells compared with adjacent tissues (ANTs) and normal cervical epithelial cells (NCEs), respectively. Patients with CC with high ILF3‑AS1 expression displayed higher survival rates compared with patients with low ILF3‑AS1 expression. Cell viability, apoptosis, migration and invasion were detected by performing Cell Counting Kit‑8, flow cytometry, wound healing and Transwell assays, respectively. Compared with the negative control (NC) group, ILF3‑AS1 overexpression significantly inhibited CC cell viability and migration, but significantly increased CC cell apoptosis. Moreover, ILF3‑AS1 overexpression significantly upregulated E‑Cadherin expression levels, but significantly downregulated N‑Cadherin and snail family transcriptional repressor 1 expression levels compared with the NC group. miR‑454‑3p expression was negatively correlated with ILF3‑AS1, and highly expressed in CC tissues and cells compared with ANTs and NCEs, respectively. PTEN, which was predicted and verified as the target gene for miR‑454‑3p, was significantly downregulated in CC tissues and cells compared with ANTs and NCEs, respectively. ILF3‑AS1 expression was positively correlated with PTEN expression, and ILF3‑AS1 overexpression partially reversed the inhibitory effect of miR‑454‑3p on PTEN expression. In conclusion, the present study indicated that ILF3‑AS1 inhibited CC cell proliferation and migration, and promoted CC cell apoptosis by inhibiting epithelial‑mesenchymal transition, and ILF3‑AS1 overexpression partially reversed the inhibitory effect of miR‑454‑3p on PTEN expression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu L, Chen R, Jiang C, Xie Q, Zhao W, Gao X and Huang H: Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis. Mol Med Rep 24: 554, 2021.
APA
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., & Huang, H. (2021). Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis. Molecular Medicine Reports, 24, 554. https://doi.org/10.3892/mmr.2021.12193
MLA
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., Huang, H."Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis". Molecular Medicine Reports 24.2 (2021): 554.
Chicago
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., Huang, H."Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis". Molecular Medicine Reports 24, no. 2 (2021): 554. https://doi.org/10.3892/mmr.2021.12193
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu L, Chen R, Jiang C, Xie Q, Zhao W, Gao X and Huang H: Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis. Mol Med Rep 24: 554, 2021.
APA
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., & Huang, H. (2021). Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis. Molecular Medicine Reports, 24, 554. https://doi.org/10.3892/mmr.2021.12193
MLA
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., Huang, H."Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis". Molecular Medicine Reports 24.2 (2021): 554.
Chicago
Zhu, L., Chen, R., Jiang, C., Xie, Q., Zhao, W., Gao, X., Huang, H."Mechanism underlying long non‑coding RNA ILF3‑AS1‑mediated inhibition of cervical cancer cell proliferation, invasion and migration, and promotion of apoptosis". Molecular Medicine Reports 24, no. 2 (2021): 554. https://doi.org/10.3892/mmr.2021.12193
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