Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2022 Volume 26 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2022 Volume 26 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Role of TRIM22 in ulcerative colitis and its underlying mechanisms

  • Authors:
    • Bin Ye
    • Zhongkai Lu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310000, P.R. China, Department of Gastroenterology, Suzhou Municipal Hospital, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, Jiangsu 215001, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 249
    |
    Published online on: June 7, 2022
       https://doi.org/10.3892/mmr.2022.12765
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ulcerative colitis (UC) is a common chronic recurrent inflammatory disease, which seriously threatens human life and health. Therefore, the present study aimed to explore the role of tripartite motif‑containing (TRIM)22 in UC and its potential mechanism. C57BL/6 mice and HT‑29 cell models of UC were constructed using 2% dextran sulphate sodium (DSS). The protein and mRNA expression levels were detected by western blotting and reverse transcription‑quantitative PCR, respectively. Cell transfection was performed to overexpress Kruppel‑like factor 2 (KLF2), or knockdown KLF2, TRIM22 and TRIM30 expression. The levels of inflammatory factors were evaluated by enzyme‑linked immunosorbent assays. Cell Counting Kit‑8 and TUNEL staining assay were employed to assess cell viability and apoptosis. Dual‑luciferase reporter assay and chromatin immunoprecipitation assay were performed to determine the binding ability of the TRIM22 promoter to KLF2. The results revealed that DSS increased the expression levels of TRIM22 in HT‑29 cells and TRIM30 in mice. Short hairpin RNA (sh)‑TRIM30 could inhibit the NF‑κB pathway, and reduce the levels of TNF‑α, IL‑6 and IFN‑γ. Furthermore, KLF2 expression was downregulated in the cell model of UC, and the luciferase assay confirmed that the 3' untranslated region of TRIM22 was a direct target of KLF2. The ChIP assay also verified the binding of KLF2 with the TRIM22 promoter. Notably, knockdown of KLF2 reversed the enhancing effects of sh‑TRIM22 on the viability of DSS‑treated HT‑29 cells. In addition, compared with in the DSS + sh‑TRIM22 group, the protein expression levels of phosphorylated (p)‑NF‑κB and p‑IκBα were increased in the DSS + sh‑TRIM22 + sh‑KLF2 group, as were the levels of TNF‑α, IL‑6 and IFN‑γ. In conclusion, TRIM22 was upregulated in DSS‑induced HT‑29 cells. TRIM22 knockdown increased DSS‑induced HT‑29 cell viability and decreased apoptosis and inflammation; this was reversed by knockdown of KLF2. These findings suggested that TRIM22 may promote disease development through the NF‑κB signaling pathway in UC and could be inhibited by KLF2 transcription.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Adams SM and Bornemann PH: Ulcerative colitis. Am Fam Physician. 87:699–705. 2013.PubMed/NCBI

2 

Keshteli AH, Madsen KL and Dieleman LA: Diet in the pathogenesis and management of ulcerative colitis; a review of randomized controlled dietary interventions. Nutrients. 11:14982019. View Article : Google Scholar

3 

Du L and Ha C: Epidemiology and pathogenesis of ulcerative colitis. Gastroenterol Clin North Am. 49:643–654. 2020. View Article : Google Scholar : PubMed/NCBI

4 

Kaenkumchorn T and Wahbeh G: Ulcerative colitis: Making the diagnosis. Gastroenterol Clin North Am. 49:655–669. 2020. View Article : Google Scholar : PubMed/NCBI

5 

da Silva BC, Lyra AC, Rocha R and Santana GO: Epidemiology, demographic characteristics and prognostic predictors of ulcerative colitis. World J Gastroenterol. 20:9458–9467. 2014. View Article : Google Scholar

6 

Huang Y, Chen J, Wong T and Liow JL: Experimental and theoretical investigations of non-Newtonian electro-osmotic driven flow in rectangular microchannels. Soft Matter. 12:6206–6213. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Nowarski R, Jackson R, Gagliani N, de Zoete MR, Palm NW, Bailis W, Low JS, Harman CC, Graham M, Elinav E and Flavell RA: Epithelial IL-18 equilibrium controls barrier function in colitis. Cell. 163:1444–1456. 2015. View Article : Google Scholar

8 

Pugliese D, Felice C, Papa A, Gasbarrini A, Rapaccini GL, Guidi L and Armuzzi A: Anti TNF-α therapy for ulcerative colitis: Current status and prospects for the future. Expert Rev Clin Immunol. 13:223–233. 2017. View Article : Google Scholar

9 

Li L, Qi Y, Ma X, Xiong G, Wang L and Bao C: TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway. Cell Biol Int. 42:1192–1199. 2018. View Article : Google Scholar

10 

Lou J, Wang Y, Zheng X and Qiu W: TRIM22 regulates macrophage autophagy and enhances Mycobacterium tuberculosis clearance by targeting the nuclear factor-multiplicity κB/beclin 1 pathway. J Cell Biochem. 119:8971–8980. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Kang C, Lu Z, Zhu G, Chen Y and Wu Y: Knockdown of TRIM22 relieves oxygen-glucose deprivation/reoxygenation-induced apoptosis and inflammation through inhibition of NF-κB/NLRP3 axis. Cell Mol Neurobiol. 41:341–351. 2021. View Article : Google Scholar

12 

Li Q, Lee CH, Peters LA, Mastropaolo LA, Thoeni C, Elkadri A, Schwerd T, Zhu J, Zhang B, Zhao Y, et al: Variants in TRIM22 that affect NOD2 signaling are associated with very-early-onset inflammatory bowel disease. Gastroenterology. 150:1196–1207. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Liu R, Zhao W, Wang H and Wang J: Long noncoding RNA LINC01207 promotes colon cancer cell proliferation and invasion by regulating miR-3125/TRIM22 axis. Biomed Res Int. 2020:12163252020. View Article : Google Scholar

14 

Chen C, Zhao D, Fang S, Chen Q, Cheng B, Fang X and Shu Q: TRIM22-mediated apoptosis is associated with bak oligomerization in monocytes. Sci Rep. 7:399612017. View Article : Google Scholar : PubMed/NCBI

15 

Ji F and Sadreyev RI: RNA-seq: Basic bioinformatics analysis. Curr Protoc Mol Biol. 124:e682018. View Article : Google Scholar

16 

Canzoneri R, Lacunza E and Abba MC: Genomics and bioinformatics as pillars of precision medicine in oncology. Medicina (B Aires). 79:(Spec 6/1). 587–592. 2019.PubMed/NCBI

17 

Wang ZL, Wang YD, Wang K, Li JA and Li L: KFL2 participates in the development of ulcerative colitis through inhibiting inflammation via regulating cytokines. Eur Rev Med Pharmacol Sci. 22:4941–4948. 2018.

18 

Liang Y, Luo J, Yang N, Wang S, Ye M and Pan G: Activation of the IL-1β/KLF2/HSPH1 pathway promotes STAT3 phosphorylation in alveolar macrophages during LPS-induced acute lung injury. Biosci Rep. 40:BSR201935722020. View Article : Google Scholar : PubMed/NCBI

19 

Vanhove W, Peeters PM, Staelens D, Anica S, der Goten JV, Cleynen I, De Schepper S, Van Lommel L, Reynaert NL, Schuit F, et al: Strong upregulation of AIM2 and IFI16 inflammasomes in the mucosa of patients with active inflammatory bowel disease. Inflamm Bowel Dis. 21:2673–2682. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Ouahed J, Gordon W, Canavan JB, Zhou HY, Du S, von Schack D, Phillips K, Wang L, Dunn WA III, Field M, et al: Mucosal gene expression in pediatric and adult patients with ulcerative colitis permits modeling of ideal biopsy collection strategy for transcriptomic analysis. Inflamm Bowel Dis. 24:2565–2578. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Cheng F, Li Q, Wang J, Zeng F, Wang K and Zhang Y: Identification of differential intestinal mucosa transcriptomic biomarkers for ulcerative colitis by bioinformatics analysis. Dis Markers. 2020:88765652020. View Article : Google Scholar : PubMed/NCBI

22 

Cardon AD, Bailey MR and Bennett BT: The animal welfare act: From enactment to enforcement. J Am Assoc Lab Anim Sci. 51:301–305. 2012.

23 

Kihara N, de la Fuente SG, Fujino K, Takahashi T, Pappas TN and Mantyh CR: Vanilloid receptor-1 containing primary sensory neurones mediate dextran sulphate sodium induced colitis in rats. Gut. 52:713–719. 2003. View Article : Google Scholar

24 

Chen Q, Fang X, Yao N, Wu F, Xu B and Chen Z: Suppression of miR-330-3p alleviates DSS-induced ulcerative colitis and apoptosis by upregulating the endoplasmic reticulum stress components XBP1. Hereditas. 157:182020. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Mehods. 25:402–408. 2001.

26 

Yamamoto-Furusho JK, Gutiérrez-Grobe Y, López-Gómez JG, Bosques-Padilla F and Rocha-Ramírez JL; Grupo del Consenso Mexicano de Colitis Ulcerosa Crónica Idiopática, : The Mexican consensus on the diagnosis and treatment of ulcerative colitis. Rev Gastroenterol Mex (Engl Ed). 83:144–167. 2018.(In English, Spanish). PubMed/NCBI

27 

Ashton JJ, Mossotto E, Stafford IS, Haggarty R, Coelho TAF, Batra A, Afzal NA, Mort M, Bunyan D, Beattie RM and Ennis S: Genetic sequencing of pediatric patients identifies mutations in monogenic inflammatory bowel disease genes that translate to distinct clinical phenotypes. Clin Transl Gastroenterol. 11:e001292020. View Article : Google Scholar

28 

Lawrence T: The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 1:a0016512009. View Article : Google Scholar

29 

Ji J, Ding K, Luo T, Zhang X, Chen A, Zhang D, Li G, Thorsen F, Huang B, Li X and Wang J: TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα. Cell Death Differ. 28:367–381. 2021. View Article : Google Scholar : PubMed/NCBI

30 

Jha P and Das H: KLF2 in regulation of NF-κB-mediated immune cell function and inflammation. Int J Mol Sci. 18:23832017. View Article : Google Scholar

31 

Zhuang T, Liu J, Chen X, Zhang L, Pi J, Sun H, Li L, Bauer R, Wang H, Yu Z, et al: Endothelial Foxp1 suppresses atherosclerosis via modulation of Nlrp3 inflammasome activation. Circ Res. 125:590–605. 2019. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ye B and Lu Z: Role of TRIM22 in ulcerative colitis and its underlying mechanisms. Mol Med Rep 26: 249, 2022.
APA
Ye, B., & Lu, Z. (2022). Role of TRIM22 in ulcerative colitis and its underlying mechanisms. Molecular Medicine Reports, 26, 249. https://doi.org/10.3892/mmr.2022.12765
MLA
Ye, B., Lu, Z."Role of TRIM22 in ulcerative colitis and its underlying mechanisms". Molecular Medicine Reports 26.2 (2022): 249.
Chicago
Ye, B., Lu, Z."Role of TRIM22 in ulcerative colitis and its underlying mechanisms". Molecular Medicine Reports 26, no. 2 (2022): 249. https://doi.org/10.3892/mmr.2022.12765
Copy and paste a formatted citation
x
Spandidos Publications style
Ye B and Lu Z: Role of TRIM22 in ulcerative colitis and its underlying mechanisms. Mol Med Rep 26: 249, 2022.
APA
Ye, B., & Lu, Z. (2022). Role of TRIM22 in ulcerative colitis and its underlying mechanisms. Molecular Medicine Reports, 26, 249. https://doi.org/10.3892/mmr.2022.12765
MLA
Ye, B., Lu, Z."Role of TRIM22 in ulcerative colitis and its underlying mechanisms". Molecular Medicine Reports 26.2 (2022): 249.
Chicago
Ye, B., Lu, Z."Role of TRIM22 in ulcerative colitis and its underlying mechanisms". Molecular Medicine Reports 26, no. 2 (2022): 249. https://doi.org/10.3892/mmr.2022.12765
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team