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Article

miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN

Retraction in: /10.3892/or.2021.8236
  • Authors:
    • Kai Liu
    • Songyang Liu
    • Wei Zhang
    • Baoxing Jia
    • Ludong Tan
    • Zhe Jin
    • Yahui Liu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatopancreatobiliary Surgery, The First Hospital, Jilin University, Changchun, Jilin, P.R. China
  • Pages: 1003-1010
    |
    Published online on: June 4, 2015
       https://doi.org/10.3892/or.2015.4030
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Abstract

MicroRNA-494 (miR-494) acts as an oncomiR and is involved in tumor development, progression and metastasis, and confers resistance to chemotherapeutic drugs by targeting a number of molecules in several human cancers. However, the function and underlying molecular mechanism of miR-494 in hepatocellular carcinoma (HCC) has not been totally elucidated. In the present study, we determined the role played by miR-494 in HCC tissues and HCC cell lines using quantitative RT-PCR (RT-qPCR). The results showed that, miR-494 was significantly upregulated in HCC tissues and HCC cell lines. Additionally, a high miR-494 expression positively correlated with tumor differentiation (P<0.01), TNM stage (P<0.01) and lymph node metastasis (P<0.01). Luciferase reporter assays confirmed that miR-494 binds to the 3'-untranslated region (3'‑UTR) of the phosphatase and tensin homolog (PTEN) mRNA and represses its translation. Functional analyses indicated that the upregulation of miR-494 promoted cell viability, migration and invasion, decreased cell apoptosis and cell cycle arrest at G1 stage, and conferred sorafenib resistance to HCC cell lines. Underexpression of PTEN by siRNA significantly attenuated the inhibitory effects of anti‑miR-494 on the proliferation, migration and invasion of liver cancer cells. Mechanistic investigations revealed that miR-494 suppressed the expression of PTEN but increased the expression of PI3K and p-Akt, which contribute to the promotion of proliferation, migration and invasion, and increased sorafenib resistance to HCC cell lines. These findings suggested that miR-494 is a potential candidate for HCC therapeutics.
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Copy and paste a formatted citation
Spandidos Publications style
Liu K, Liu S, Zhang W, Jia B, Tan L, Jin Z and Liu Y: miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236. Oncol Rep 34: 1003-1010, 2015.
APA
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., & Liu, Y. (2015). miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236. Oncology Reports, 34, 1003-1010. https://doi.org/10.3892/or.2015.4030
MLA
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., Liu, Y."miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236". Oncology Reports 34.2 (2015): 1003-1010.
Chicago
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., Liu, Y."miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236". Oncology Reports 34, no. 2 (2015): 1003-1010. https://doi.org/10.3892/or.2015.4030
Copy and paste a formatted citation
x
Spandidos Publications style
Liu K, Liu S, Zhang W, Jia B, Tan L, Jin Z and Liu Y: miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236. Oncol Rep 34: 1003-1010, 2015.
APA
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., & Liu, Y. (2015). miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236. Oncology Reports, 34, 1003-1010. https://doi.org/10.3892/or.2015.4030
MLA
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., Liu, Y."miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236". Oncology Reports 34.2 (2015): 1003-1010.
Chicago
Liu, K., Liu, S., Zhang, W., Jia, B., Tan, L., Jin, Z., Liu, Y."miR-494 promotes cell proliferation, migration and invasion, and increased sorafenib resistance in hepatocellular carcinoma by targeting PTEN Retraction in /10.3892/or.2021.8236". Oncology Reports 34, no. 2 (2015): 1003-1010. https://doi.org/10.3892/or.2015.4030
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