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Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo

  • Authors:
    • Yang Yang
    • Hui Luo
    • Kaiyuan Hui
    • Yali Ci
    • Kejian Shi
    • Ge Chen
    • Lei Shi
    • Caimin Xu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), School of Basic Medicine, Peking Union Medical College (PUMC), Beijing 100005, P.R. China, Shenzhen University Health Science Center, Shenzhen 518060, P.R. China, Department of General Surgery, PUMC Hospital, CAMS, Beijing 100730, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1255-1264
    |
    Published online on: December 10, 2015
       https://doi.org/10.3892/or.2015.4484
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Abstract

In the present study, we aimed to investigate the relationship between autophagy and apoptosis in selenite‑treated colorectal cancer (CRC) cells. The effects of selenite on HCT116 and SW480 cell apoptosis were investigated with an Annexin V/propidium iodide (PI) double staining kit by flow cytometry. The punctate of LC3 protein following treatment with selenite was observed by a laser scanning confocal microscope and by transmission electron microscopy. Using western blot assays, we detected the apoptotic and autophagic markers in both CRC cells and mouse xenograft tumor models. We found that sodium selenite induced autophagy in the two CRC cell lines. Consistent with the in vitro results, we observed that the expression of autophagy marker LC3 was increased. Finally, we discovered that modulation of reactive oxygen species by MnTMPyP inhibited autophagy, while H2O2 activated autophagy. These results help to elucidate the anticancer effect of selenium, providing further evidence to exploit novel anticancer drugs targeting selenium.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Luo H, Hui K, Ci Y, Shi K, Chen G, Shi L and Xu C: Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncol Rep 35: 1255-1264, 2016.
APA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G. ... Xu, C. (2016). Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncology Reports, 35, 1255-1264. https://doi.org/10.3892/or.2015.4484
MLA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35.3 (2016): 1255-1264.
Chicago
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35, no. 3 (2016): 1255-1264. https://doi.org/10.3892/or.2015.4484
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Luo H, Hui K, Ci Y, Shi K, Chen G, Shi L and Xu C: Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncol Rep 35: 1255-1264, 2016.
APA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G. ... Xu, C. (2016). Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncology Reports, 35, 1255-1264. https://doi.org/10.3892/or.2015.4484
MLA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35.3 (2016): 1255-1264.
Chicago
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35, no. 3 (2016): 1255-1264. https://doi.org/10.3892/or.2015.4484
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