The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma

  • Authors:
    • Zhenkuan Xu
    • Jie Gong
    • Chuanwei Wang
    • Yunyan Wang
    • Yan Song
    • Wenzhe Xu
    • Zhiguo Liu
    • Yuguang Liu
  • View Affiliations

  • Published online on: August 25, 2016     https://doi.org/10.3892/or.2016.5046
  • Pages: 2236-2244
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Abstract

Previous studies indicated that phosphoglycerate mutase 1 (PGAM1) is involved in many cancer types and promotes breast cancer progression. However, the role of PGAM1 in glioma remains unclear. The present study aimed to investigate the association of PGAM1 expression with glioma grade and the role of PGAM1 in proliferation, apoptosis, migration and invasion of glioma cells. The mRNA and protein expression of PGAM1 was analysed in glioma tissues and normal brain tissues. The expression of PGAM1 was examined further by immunohistochemical analysis. In addition, we inhibited the expression of PGAM1 in glioma cell line by siRNA to evaluate its role in glioma proliferation, apoptosis, migration and invasion. The mRNA and protein expression of PGAM1 was significantly greater in glioma than normal brain tissues. PGAM1 expression was associated with the WHO grade of glioma. siRNA knockdown of PGAM1 significantly inhibited glioma cell proliferation, promoted glioma cell apoptosis, induced S phase cell cycle arrest and inhibited glioma cell migration and invasion in vitro. PGAM1 may be associated with the grade of glioma and be involved in the biological behavior of glioma cells. PGAM1 might be a novel therapeutic target in glioma.

Introduction

Gliomas are the most common primary brain tumors and account for 40–50% of primary intracranial neoplasms (1). They can occur anywhere in the central nervous system but primarily in the brain, and originate from abnormally proliferating glial cells, which normally supply nutrition and protection to neurons in the central nervous system (2). The etiology and pathogenesis of gliomas still remain obscure. Despite the advances in therapeutic approaches, the treatment offers limited help to prolong survival (3). In view of the overall poor outcome with current therapies, a better understanding of glioma etiology is crucial for future development of more effective treatments to cure this rapidly progressing disease.

Phosphoglycerate mutase 1 (PGAM1) catalyzes the conversion of 3-phosphoglycerate (3-PG) to 2-phosphoglycerate (2-PG) to release energy during glycolysis. Proteome reactivity profiling was used to determine the drug target against breast cancer, and PGAM1 was found a potential metabolic enzyme participating in breast carcinogenesis (4). Several studies demonstrated that PGAM1 activity was increased in a variety of human cancers, including lung (5), breast (4), prostate cancer (6), hepatocellular carcinoma (7), colorectal cancer (8), oral squamous cell carcinoma (9), and esophageal squamous cell carcinoma (10) and also associated with virus infection (11) and spermatogenic dysfunction (12). Previous reports have described that targeting PGAM1 by a PGAM1-derived inhibitory peptide or PGAM inhibitor MJE3 could attenuate breast cancer cell proliferation (4,13). However, the association of PGAM1 with glioma grade and the role of PGAM1 in glioma are poorly investigated.

In the present study, we aimed to evaluate the expression patterns, the clinical roles and the functions of PGAM1 in human gliomas, as a possible diagnostic biomarker and therapeutic target for glioma.

Materials and methods

Patients and tissue specimens

All 124 glioma specimens were obtained from patients with primary glioma who underwent surgical treatment between July 2012 and May 2014 at Qilu Hospital; 20 normal brain tissue samples were collected from patients undergoing surgery for epilepsy and were reviewed to verify the absence of a tumor. For all patients, the histological types and grade of gliomas were evaluated by two experienced pathologists according to the 2007 WHO Classification of Tumors of the Central Nervous System (14). All patients had a well-documented clinical history. The study protocol was approved by the Ethics Committee of Qilu Hospital (Shandong, China). Informed consent was obtained from all patients included in the study.

RNA isolation and real-time PCR

Total RNA was extracted from frozen specimens by using TRIzol reagent (Invitrogen/Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions. cDNA was generated by reverse transcription of 1 µg total RNA with primer oligonucleotides. qPCR reactions were conducted in a 10-µl reaction volume with SYBR-Green I, with a 1:25 dilution of the cDNA and 20 nM primers. β-actin was a reference gene. The primers were designed by the use of Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA, USA) from mRNA sequences in the GenBank database. Primer sequences were for PGAM1 (sense: 5′-GTGCAGAAGAGAGCGATCCG-3′ and antisense: 5′-CGGTTAGACCCCCATAGTGC-3′); β-actin (sense: 5′-CGTTGACATCCGTAAAGACC-3′ and antisense: 5′-TAGAGCCACCAATCCACACA-3′). Calculation of PGAM1 mRNA levels was based on cycle threshold (Ct) values and determined by relative quantification with β-actin as a normalizing gene according to the following equation: 2−ΔCt [ΔCt = Ct (PGAM1) − Ct (β-actin)]. All experiments were performed in triplicate.

Western blot analysis

Briefly, cultured cells and tissues were lysed in RIPA buffer (Beyotime Institute of Biotechnology, Haimen, China) containing 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail with 1 mM phenylmethanesulfonyl fluoride (Beyotime Institute of Biotechnology). Lysates were centrifuged at 12,000 × g for 15 min and the protein concentration of the supernatant was quantified by the BCA method. An amount of 20 µg protein from each sample was separated by 10% SDS-PAGE and transferred to a polyvinylidene difluoride membranes. After blocking with 5% non-fat milk in phosphate-buffered saline (PBS) Tween-20 for 1 h at room temperature, the membranes were incubated with primary antibody overnight. The membrane was rinsed and washed 3 times with TBS containing 0.1% Tween-20, then incubated with horseradish peroxidase-conjugated secondary antibody for 2 h at room temperature. Immunoreactive bands were visualized by using the Immobilon Western Chemiluminescent HRP Substrate kit (Millipore). Immunoreactive labeling was analyzed by the use of ImageJ 1.44 (National Institutes of Health, Bethesda, MD, USA) and standardized against β-actin protein level.

Antibody for PGAM1 was from Santa Cruz Biotechnology (Dallas, TX, USA); antibodies for Bax, Bcl-2, matrix metalloproteinase 2 (MMP2)/9 and cleaved caspase-3 were from Cell Signaling Technology (Danvers, MA, USA).

Immunohistochemistry

All samples were fixed with 10% buffered formalin and embedded in paraffin. Slices of paraffin tissues were cut as 4-µm serial sections. Slides were stained according to the manufacturer's protocol. In brief, slides were deparaffinized by using xylene and rehydrated through an ethanol series to water. The dewaxed slides underwent antigen retrieval in citrate buffer for 30 min by microwaving and then cooled to room temperature to expose antigen epitopes. An amount of 3% hydrogen peroxide was added to slides to inhibit endogenous peroxidase activity. The slides were blocked with 10% normal goat serum for 1 h at 37°C, then incubated with primary rabbit anti-human PGAM1 polyclonal antibody overnight at 4°C, then anti-rabbit secondary antibody. Finally, DAB was used as chromogen, and the slides were counterstained with hematoxylin. Slides were dehydrated and mounted with neutral balsam according to the laboratory protocol. Staining with PBS instead of the primary antibody was a negative control.

Evaluation of immunohistochemistry

Each stained slide was simultaneously scored by two independent pathologists who were blinded to the clinical information. We selected 10 low-power fields randomly, and counted cells at high-power field. The percentage of PGAM1-positive cells was scored as 4 categories: 0, no staining; 1, <25% cells; 2, 25–75% cells; and 3, >75% cells. The intensity of positive staining was scored as 4 grades: 0, negative; 1, weak; 2, moderate; and 3, strong. The final staining score was then obtained (ranging from 0 to 9) by the multiplication of the intensity and percentage scores. The staining pattern of slides was scored as 0, −; 1–3, +; 4–6, ++; and 6–9, +++. In addition, samples with scores up to ++ were considered positive.

Cell culture

The human glioma cell line (U87) was obtained from the Chinese Academy of Science and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin at 37°C in a humidified atmosphere containing 5% CO2 in 60-mm flasks.

Transfection

When cultured U87 cells reached 90% confluence, they were transfected with siRNA targeting PGAM1. The siRNA sequences were as follows: PGAM1-siRNA: 5′-CGACUGGUAUUCCCAUUGUTT-3′ and 5′-ACAAUGGGAAUACCAGUCGTT-3′; Negative scramble control sequences: 5′-UUCUCCGAACGUGUCACGUTT-3′ and 5′-ACGUGACACGUUCGGAGAATT-3′. The RNA duplexes were synthesized by Shanghai GenePharma Co., Ltd. (Shanghai, China). Transfection of siRNA involved use of Lipofectamine 2000 (Invitrogen/Life Technologies) according to the manufacturer's instructions. The efficiency of gene silencing was confirmed by assaying PGAM1 protein levels.

Cell proliferation assay

Proliferation of U87 cells was determined by standard MTT assay (Beyotime Institute of Biotechnology). Briefly, MTT solution (20 µl, 5 mg/ml) was added into each well for incubation at 37°C for 4 h. Then the solution was removed by aspiration, the insoluble formazan crystals were dissolved in 150 µl/well dimethyl sulfoxide (DMSO), and absorbance at 490 nm was measured by the use of a Varioskan Flash spectral scanning multimode reader (Thermo Electron Oy, Vantaa, Finland). The spectrophotometer was calibrated to zero absorbance with culture medium without cells. The percentage of cell survival was determined by comparing the average absorbance of treated cells to that of untreated cells. The experiment was repeated at least 3 times.

Cell apoptosis analysis

Cell apoptosis was evaluated by the use of an Annexin V-FITC/PI kit (Invitrogen/Life Technologies). Briefly, after treatment, attached cells were collected and washed with PBS twice. An amount of 400 µl binding buffer, 5 µl Annexin V FITC and 5 µl PI was successively added to the cell suspension. After 15 min of incubation in the dark, cells underwent flow cytometry (Becton-Dickinson, San Jose, CA, USA). At least 3 independent experiments were carried out.

Cell cycle analysis

Cell cycle analysis was evaluated with a PI staining kit (Beyotime Institute of Biotechnology). Briefly, ~12×105 U87 cells were seeded in a 6-well plate, allowed to attach for 48 h, then cells were collected by the trypsin method, washed with PBS, and fixed overnight at 4°C in 70% ethanol. Fixed cells were washed with PBS; 20 µl RNase A was added and incubated at 37°C for 30 min to hydrolyze RNA, then cells were stained with PI in the dark for another 30 min, and the cell cycle was evaluated by flow cytometry. The experiments were repeated at least 3 times independently.

In vitro cell migration and invasion Boyden chamber assay with some modifications

Briefly, 5×104 siRNA-transfected cells in 0.1 ml of serum-free DMEM were added to the wells of an 8-µm pore membrane Boyden chamber (Corning, Inc., Corning, NY, USA) uncoated (for the migration assay) or coated with Matrigel (BD Biosciences; for the invasion assay). The bottom chamber contained 10% FBS in DMEM, which served as a chemoattractant. Cells were allowed to invade for 48 h, and the cells that had not penetrated the filters were removed from the filters with cotton swabs. Chambers were fixed for 20 min at room temperature with 4% formaldehyde in PBS, stained in 0.1% crystal violet for 30 min, and rinsed in water. Cells that migrated to the bottom surface of the filter were counted under a light microscope. Assays were performed 3 times with triplicate wells.

Statistical analysis

Data are expressed as mean ± SEM. SPSS 18.0 was used for analysis. Data were analyzed by two-tailed Student's t-test and one-way ANOVA. Chi-square test was used to analyze categorical data. P<0.05 was considered statistically significant.

Results

PGAM1 is upregulated in human glioma tissues

Expression of PGAM1 was compared at both transcriptional and translational levels between glioma tissues and normal brain tissues. We analyzed the mRNA expression of PGAM1 in glioma tissues and normal brain tissues to assess the role of PGAM1 in the malignant progression of glioma. PGAM1 mRNA level was higher in low-grade and high-grade glioma than normal brain tissue (Fig. 1A) and PGAM1 protein expression was higher in tumor tissue (Fig. 1B). Immunohistochemical staining was used to detect the expression of PGAM1 in paraffin-embedded tissues (Fig. 2). PGAM1 was predominantly confined to the cytoplasm of tumor cells. PGAM1-positive expression was present in 74.2% (92/124) of glioma tissues and in 25% (5/20) of normal brain tissues (Table I).

Table I

PGAM1 expression in glioma tissues and normal brain tissues.

Table I

PGAM1 expression in glioma tissues and normal brain tissues.

TissuesTotal−/+ (%)++/+++ (%)P-value
Normal2015 (75)5 (25)
Glioma12432 (25.8)92 (74.2)<0.05
The protein expression of PGAM1 in tissue by glioma grade

PGAM1 protein expression was associated with glioma grade (Fig. 3). The expression of PGAM1 was 56.3% in grade II glioma, 79.0% in grade III glioma and 81.5% in grade IV glioma on immunohistochemistry (Table II). PGAM1 protein expression was higher in high-grade glioma than low-grade glioma tissue. The significant upregulation of PGAM1 in glioma tissues indicated that PGAM1 may function as a tumor promoter in glioma.

Table II

Association between PGAM1 protein level and clinicopathological features of glioma patients.

Table II

Association between PGAM1 protein level and clinicopathological features of glioma patients.

VariablesTotal−/+ (%)++/+++ (%)P-value
Age (years)
 <506736 (53.7)31 (46.3)
 ≥505729 (50.9)28 (49.1)0.751 >0.05
Gender
 Male6935 (50.7)34 (49.3)
 Female5530 (54.6)25 (45.4)0.672 >0.05
WHO grade
 II3214 (43.7)18 (56.3)
 III388 (21.0)30 (79.0)<0.05 III vs. II
 IV5410 (18.5)44 (81.5)<0.05 IV vs. II
Downregulation of PGAM1 inhibits glioma cell proliferation in vitro

We transiently transfected PGAM1 siRNA into U87 glioma cells to investigate the potential function of PGAM1 and confirmed the downregulation of PGAM1 protein level with PGAM1 siRNA transfection as compared with controls (Fig. 4). Cell proliferation ability was analyzed by MTT assay at 48 h after transfection in U87 cells. Cell proliferation was reduced with PGAM1 siRNA transfection as compared with negative control transfection in U87 cells (Fig. 5). Therefore, downregulation of PGAM1 inhibited glioma cell growth in vitro.

Knockdown of PGAM1 induces apoptosis in U87 cells

Cell apoptosis was measured by Annexin V FITC/PI staining to further explore the mechanism of PGAM1 modulating glioma cell growth (Fig. 6A). siRNA knockdown of PGAM1 significantly increased cell apoptosis as compared with controls. After transfection for 48 h, the early apoptotic cells (right lower domain of the fluorocytogram) and late apoptotic cells (right upper domain of the fluorocytogram) increased from 0.83±0.67 and 2.47±0.39 to 32.0±4.26 and 6.69±3.11%, respectively; thus, the total mean apoptotic rate reached 42.9% with PGAM1 siRNA transfection (Fig. 6B). Apoptosis regulators were further examined (Fig. 6C). The ratio of Bax to Bcl-2 expression increased in U87 cells with PGAM1 siRNA knockdown (Fig. 6D). Additionally, cleaved caspase-3 activity was significantly upregulated in PGAM1 siRNA-transfected glioma cells compared with the control (Fig. 6E).

Silencing of PGAM1 induces cell cycle arrest in U87 cells

Knockdown of PGAM1 significantly increased the cell population in the S phase and decreased that in the G2/M phase (Fig. 7A). The controls had a mean of 13.48±2.03% cells in the S phase; after siRNA transfection for 48 h, the rates increased to 46.86±2.73% (Fig. 7C), and the rates of cells in the G2/M phase decreased from 22.16±0.95 to 8.61±0.37% (Fig. 7B). Therefore, PGAM1 siRNA knockdown induced S-phase cell cycle arrest in U87 cells.

PGAM1 knockdown inhibited glioma cell migration and invasion

To test the effect of PGAM1 on glioma cell mobility, we performed Transwell migration and invasion assays in U87 cells at 48 h after PGAM1 siRNA transfection. The migration ability of transfected U87 cells was decreased (Fig. 8A and B). Furthermore, the invasion ability of transfected cells was inhibited as compared with controls (Fig. 8A and C). Since MMP2/9 play important roles in tumor cell migration and invasion, we examined the effect of PGAM1 on their expression. PGAM1 siRNA knockdown downregulated MMP2/9 protein levels in U87 cells (Fig. 8D–F). PGAM1 knockdown may significantly inhibit glioma cell migration and invasion in vitro.

Discussion

Glioma is one of the most common adult primary central nervous system tumors, with a median survival of only 14.6 months after diagnosis (14,15). Gliomas can be classified into WHO grades I–IV based on malignant behavior (14). Standard treatment includes surgical operation followed by radiotherapy and chemotherapy (16). Glioma is rarely curable despite these treatments. Since the major barrier to effective treatment of glioma is their highly invasive nature, special attention needs to be paid to the molecular determinants regulating their malignant behavior. In this study, we demonstrated upregulated mRNA and protein levels of PGAM1 in glioma tissues. Importantly, the expression of PGAM1 was significantly associated with WHO grade of glioma. siRNA knockdown of PGAM1 inhibited glioma cell growth, migration and invasion, and induced cell apoptosis and cell cycle arrest. PGAM1 might be a novel therapeutic target in glioma.

The Warburg effect is a property of cancer cells whereby cancer cells maintain a high rate of aerobic glycolysis even under the high-oxygen (20%) conditions of normal tissue culture (17). The effect helps cancer cells generate more ATP more quickly than normal cells, which mainly rely on oxidative phosphorylation. As well, tumor tissue accumulates more glucose than does normal tissue, because cancer cells make use of large amounts of glucose as a molecular source for anabolic biosynthesis of macromolecules, which are necessary for cancer cell proliferation (18). The idea that tumors have a particular metabolic phenotype that is associated with increased glycolysis is supported by molecular and functional data (19). Microarray datasets collected from several studies have consistently shown most of the genes involved in glucose transport and glycolysis are upregulated in different types of tumors (20,21). Glucose transporters are overexpressed in hepatocarcinomas, breast cancer, neuroendocrine carcinomas, lymphoblastic leukemia and others (2225). In mesenchymal glioma stem cells, the glycolysis pathway is highly upregulated, involving aldehydedehydrogenase 1A3, which creates heterogeneous cellular populations (26). A recent study demonstrated that the activity of oxidative phosphorylation complexes and citrate synthase was gradually decreased by tumor grade in glioma as compared with normal brain tissue (27). This shift in cellular energy production from oxidative phosphorylation to glycolysis is a result of the glioma adapting to the surrounding environment. Several mechanisms were involved in the downregulation of oxidative phosphorylation in tumor cells. Lack of vascularization in tumors causes severe hypoxia, which leads to a compensatory upregulation of glycolysis in tumors. Genetic inactivation of p53 gene, a regulator of oxidative phosphorylation, or activation of oncogenes can result in a secondary decrease in oxidative phosphorylation (2831). Loss of function of components of oxidative phosphorylation has been demonstrated in pheochromocytomas and paragangliomas (32). In this context, glycolysis plays a significant role in survival and growth of cancer cells (33). Therefore, intervening in glycolysis could result in remarkable inhibition of cell growth and induction of cell death (13,34).

PGAM1 is involved in the Warburg effect and catalyzes the conversion of 3-PG to 2-PG, a crucial step in glycolysis (35). PGAM1 controls intracellular 3-PG and 2-PG levels and is important for anabolic biosynthesis in cells. PGAM1 knockdown increases 3-PG level and reduces 2-PG level. PGAM1 enzyme activity strikes a balance between 3-PG and 2-PG levels, which coordinates glycolysis and biosynthesis to promote cancer cell proliferation (36).

PGAM1 activity is upregulated in many cancers. Targeting PGAM1 by a small molecule inhibitor inhibited cancer cell proliferation and tumor growth and altered 3-PG and 2-PG levels in primary leukemia cells from human patients, thereby leading to attenuated leukemia cell proliferation (4,13,36). A recent study revealed that Y26 phosphorylation of PGAM1 enhanced PGAM1 activity by stabilising the active conformation of PGAM1, which promoted tumor growth (37). Protein expression profile analysis revealed that overexpression of PGAM1 was associated with poor survival of patients with lung adenocarcinoma (5). Another study suggested that overexpression of PGAM1 was strongly correlated with poor differentiation and decreased survival rates in hepatocellular carcinoma (7). A recent study also demonstrated that PGAM1 knockdown had a marked survival benefit in mice with intracranial xenografts (38). These studies suggest that PGAM1 probably participates in gliomagenesis. Our results show that PGAM1 expression was significantly upregulated in glioma tissues compared with human brain tissues. Furthermore, PGAM1 expression increased with increasing pathological glioma grade. Our result is consistent with a recent report of protein expression profiles in gliomas (39). Taken together, these results indicate that PGAM1 might function as a tumor promoter in glioma.

We confirmed the function of PGAM1 in glioma cells. MTT assay showed that cell proliferation was inhibited with transfection of PGAM1 siRNA into U87 cells. Moreover, siRNA knockdown of PGAM1 expression significantly induced glioma cell apoptosis in vitro by upregulating Bax expression, downregulating Bcl-2 expression, and activating the caspase-3 signal in glioma cells. In addition, siRNA knockdown of PGAM1 induced S-phase cell cycle arrest and decreased the rate of cells in the G2/M phase in U87 cells. The highly invasive nature of glioma cells renders them incurable by localized therapy including surgery and radiotherapy, thereby leading to poor patient survival (40,41). Thus, using RNA interference, we defined a specific role for PGAM1 in glioma migration and invasion. After transfection with PGAM1 siRNA for 48 h, the rates of cell migration and invasion were significantly reduced compared with those in control cells, which suggests that PGAM1 knockdown is deleterious for U87 cell migration and invasion. Importantly, PGAM1 knockdown suppressed the protein levels of MMP2/9, markers for cell invasion, which may be important for inhibiting the invasive potential of U87 cells. Our data strongly suggest that PGAM1 plays an important role in glioma development and progression. Additional molecular and functional studies of PGAM1 in glioma cells are needed.

In conclusion, previous studies suggest that targeting PGAM1 by siRNA or with a small molecule inhibitor PGMI-004A attenuates cancer cell proliferation (7,36). In agreement with these observations, we found that the expression of PGAM1 is important in glioma cell proliferation. PGAM1 knockdown efficiently inhibited glioma cell migration and invasion and induced cell cycle arrest and apotosis in vitro. PGAM1 might be a promising therapy in clinical treatment of glioma, which heavily relies on aerobic glycolysis.

Acknowledgments

The present study was supported by the Natural Scientific Foundation of China (no. 81141088), the Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (no. 2004BS02010), the Jinan Youth Technology Star Plan (20120137), the Shandong Excellent Youth Scientist Research Fund (BS2012YY022), and the Shandong University Innovation Fund (2012TS135). Thanks to Laura Smales for the English editing.

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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Z, Gong J, Wang C, Wang Y, Song Y, Xu W, Liu Z and Liu Y: The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma. Oncol Rep 36: 2236-2244, 2016.
APA
Xu, Z., Gong, J., Wang, C., Wang, Y., Song, Y., Xu, W. ... Liu, Y. (2016). The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma. Oncology Reports, 36, 2236-2244. https://doi.org/10.3892/or.2016.5046
MLA
Xu, Z., Gong, J., Wang, C., Wang, Y., Song, Y., Xu, W., Liu, Z., Liu, Y."The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma". Oncology Reports 36.4 (2016): 2236-2244.
Chicago
Xu, Z., Gong, J., Wang, C., Wang, Y., Song, Y., Xu, W., Liu, Z., Liu, Y."The diagnostic value and functional roles of phosphoglycerate mutase 1 in glioma". Oncology Reports 36, no. 4 (2016): 2236-2244. https://doi.org/10.3892/or.2016.5046