MicroRNA-194 suppresses prostate cancer migration and invasion by downregulating human nuclear distribution protein

  • Authors:
    • Qi Kong
    • Xu-Shen Chen
    • Tian Tian
    • Xiang-You Xia
    • Peilin Xu
  • View Affiliations

  • Published online on: December 8, 2016     https://doi.org/10.3892/or.2016.5305
  • Pages: 803-812
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Abstract

Human NudC nuclear distribution protein (hNUDC) is differentially expressed between normal and cancer cells. Based on its marked altered expression and its roles in modulating cell division, cytokineses and migration, a detailed understanding of the mechanisms regulating hNUDC expression in cancer cells is critical. In this study, we identified miR-194 as a downstream target of hNUDC and linked its expression to reduced metastatic capacity and tumorigenicity of prostate cancer (PCa) cells. Using miRNA target prediction programs, hNUDC mRNA was found to contain a potential binding site for miR-194 within its 3'UTR. A Reporter assay confirmed that post-transcriptional regulation of hNUDC was dependent on the miR-194 binding site. Forced expression of miR-194 in PCa cell lines, PC-3 and DU-145, led to a decrease in the mRNA and protein levels of hNUDC. Overexpression of miR-194 in these cells inhibited cell migration and invasion, and induced multinucleated cells. Our data showed that hNUDC knockdown by siRNA significantly reduced the migration and invasion in the PC-3 and DU-145 cells, phenocopying the results of miR-194 overexpression. Furthermore, lentivirus-mediated stable expression of miR-194 in PCa cells reduced the ability of colony formation as detected by a soft agar assay and exhibited significantly less tumorigenic ability in vivo. Our results suggest a novel role for miR-194 in effectively controlling cell metastatic processes in PCa cells via the regulation of hNUDC expression.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kong Q, Chen X, Tian T, Xia X and Xu P: MicroRNA-194 suppresses prostate cancer migration and invasion by downregulating human nuclear distribution protein. Oncol Rep 37: 803-812, 2017.
APA
Kong, Q., Chen, X., Tian, T., Xia, X., & Xu, P. (2017). MicroRNA-194 suppresses prostate cancer migration and invasion by downregulating human nuclear distribution protein. Oncology Reports, 37, 803-812. https://doi.org/10.3892/or.2016.5305
MLA
Kong, Q., Chen, X., Tian, T., Xia, X., Xu, P."MicroRNA-194 suppresses prostate cancer migration and invasion by downregulating human nuclear distribution protein". Oncology Reports 37.2 (2017): 803-812.
Chicago
Kong, Q., Chen, X., Tian, T., Xia, X., Xu, P."MicroRNA-194 suppresses prostate cancer migration and invasion by downregulating human nuclear distribution protein". Oncology Reports 37, no. 2 (2017): 803-812. https://doi.org/10.3892/or.2016.5305