Chloroquine inhibits cell growth in human A549 lung cancer cells by blocking autophagy and inducing mitochondrial‑mediated apoptosis

  • Authors:
    • Likun Liu
    • Cuicui Han
    • Haitao Yu
    • Wenbin Zhu
    • Hongxia Cui
    • Lihong Zheng
    • Chunjing Zhang
    • Liling Yue
  • View Affiliations

  • Published online on: April 12, 2018     https://doi.org/10.3892/or.2018.6363
  • Pages: 2807-2816
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Abstract

Chloroquine (CQ) has been revealed to exhibit antitumor activity in several human tumors including lung cancer as mono‑ or add‑on therapy. The antitumor effect of CQ appears to depend on the tumor type, stage and genetic context. Few studies have focused on the mechanism concerning the antitumor effect of CQ monotherapy and the cause and effect relationship among autophagy, apoptosis and CQ in human lung A549 cells. Therefore, the present study aimed to identify the antitumor effect of CQ monotherapy and analyze the possible mechanism. In the present study, we demonstrated that CQ suppressed human A549 cell growth in a dose‑ and time‑dependent manner. CQ‑mediated growth inhibition in A549 cells was characterized by the targeting of the PI3K/AKT pathway, thus, inducing mitochondria‑mediated apoptosis at relatively higher concentrations by downregulating Bcl‑2 expression, increasing the expression level of Bax, decreasing mitochondrial membrane potential, releasing cytochrome c from the mitochondria into the cytosol, activating caspase‑3 and cleaving PARP. Collectively, these findings may offer a new rationale for using CQ as a lung cancer therapy drug in clinical practice.
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June-2018
Volume 39 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu L, Han C, Yu H, Zhu W, Cui H, Zheng L, Zhang C and Yue L: Chloroquine inhibits cell growth in human A549 lung cancer cells by blocking autophagy and inducing mitochondrial‑mediated apoptosis. Oncol Rep 39: 2807-2816, 2018.
APA
Liu, L., Han, C., Yu, H., Zhu, W., Cui, H., Zheng, L. ... Yue, L. (2018). Chloroquine inhibits cell growth in human A549 lung cancer cells by blocking autophagy and inducing mitochondrial‑mediated apoptosis. Oncology Reports, 39, 2807-2816. https://doi.org/10.3892/or.2018.6363
MLA
Liu, L., Han, C., Yu, H., Zhu, W., Cui, H., Zheng, L., Zhang, C., Yue, L."Chloroquine inhibits cell growth in human A549 lung cancer cells by blocking autophagy and inducing mitochondrial‑mediated apoptosis". Oncology Reports 39.6 (2018): 2807-2816.
Chicago
Liu, L., Han, C., Yu, H., Zhu, W., Cui, H., Zheng, L., Zhang, C., Yue, L."Chloroquine inhibits cell growth in human A549 lung cancer cells by blocking autophagy and inducing mitochondrial‑mediated apoptosis". Oncology Reports 39, no. 6 (2018): 2807-2816. https://doi.org/10.3892/or.2018.6363